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Chemicals
MIDSCI™ is Your Trusted Source for High-Quality Lab Chemicals and Reagents
Looking for reliable chemicals to power your research? MIDSCI™ delivers a full range of high-purity reagents, solvents, and buffers designed for life science, pharmaceutical, microbiology, and organic chemistry workflows. From essential acids and bases to specialized biochemicals, growth media, and DNA kits, our inventory ensures precision, consistency, and compliance, backed by Certificates of Analysis (COAs) and Safety Data Sheets (SDS).
Explore PR1MA™ Chemicals, our premium line of USP- and ACS-grade chemicals
Perfect for molecular biology, plant science, and cellular research, PR1MA™ products offer full traceability and documentation for your peace of mind.
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Vion ACS Reagent Grade Water
Vion ACS Reagent grade water is guaranteed to meet the purity standards set by the American Chemical Society (ACS). It is a high-quality, highly purified water with minimal contaminants, and is ideal for use in laboratory settings for sensitive chemical analyses, like liquid chromatography and UV-Visible spectrophotometry, and reactions where extreme purity is required.
Specifications
| Conductivity | ≤ 2.0 uS / cm |
| Silicate (as SiO2) | ≤ 0.01 ppm |
| Heavy Metals (as Pb) | ≤ 0.01 ppm |
| Substances Reducing Permanganate | Passes Test |
| Chloride (Cl) | ≤ 0.4 ppm |
| Nitrate (NO3) | ≤ 0.4 ppm |
| Phosphate (PO4) | ≤ 1 ppm |
| Sulfate (SO4) | ≤ 1 ppm |
| UV A300 nm | ≤ 0.005 |
| UV A254 nm | ≤ 0.005 |
| UV A210 nm | ≤ 0.010 |
| UV A200 nm | ≤ 0.010 |
| LC Absorbance | Passes Test |
| LC Gradient Elution | Passes Test |
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Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
RWD Cell Separation Assembly Kit
Applications include immunology research, oncology research, neuroscience, stem cell research, and cell therapy.
RWD magnetic microbead cell separation products consist of Nano-Bead Cell Separation Platform and Nano-Beads (Column Based and Column free). Nano-sized magnetic beads do not need elution, and the separated cells can be directly used in downstream experiments such as flow cytometry, cell culture, single-cell sequencing, and more.
- High purity, high viability, and high recovery rate
- Obtained cells are compatible with downstream applications
- Mild, non-toxic, biodegradable
Kit Includes
- 1 box of LarSep Columns (25/pk)
- 1 LSC Separator (single channel)
- 1 Separation Stand
Work Flow
- Antibody incubation: Incubate the antibody with cell suspension at 4ºC for 10-15 mins.
- Microbead incubation: Incubate the magnetic microbeads with cell suspension at 4ºC for 10-15 mins.
- Magnetic separation: Put the separation column into the magnetic field and add the cell suspension into the column.
- Only cells labeled with magnetic beads will be captured in the column.
- Elution: Remove the column from the magnetic field and add a buffer to elute the positive cells.
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
RWD Human Cell Separation Kits
CD3+ Cell Separation Kit - K1201-10
For positive selection or depletion of CD3+ T cells from peripheral blood, thymus, lymphoid, and tumor tissue.
CD4+ Cell Separation Kit - K1202-10
For positive selection or depletion of T helper cells (Th) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.
CD8+ Cell Separation Kit - K1203-10
For positive selection or depletion of cytotoxic T cells (Tc) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.
CD14+ Cell Separation Kit - K1204-10
For positive selection or depletion of monocytes from human peripheral blood, umbilical cord blood, spleen, lymph nodes, and other samples.
CD45+ Cell Separation Kit - K1205-10
For positive selection or depletion of white blood cells from human peripheral blood and lymphoid tissue samples.
CD19+ Cell Separation Kit - K1206-10
For positive selection or depletion of B cells from human peripheral blood, lymph nodes, bone marrow, and other single-cell suspensions.
Human NK Cell Isolation Kit - K1207-10
For negative selection or depletion of NK cells from single cell suspension of human peripheral blood. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and red blood cells are labeled by biotin antibodies coupled to magnetic beads. High purity NK cells are obtained by isolation of magnetic bead labeled cells.
*All kits are for research use only.
Specifications
| Item # | Cell Type | Size | Capacity |
| K1201-10 | T Cells | 2 x 1 mL | For 1 x 10^9 Total Cells |
| K1202-10 | T Helper Cells | 2 x 1 mL | |
| K1203-10 | Cytotoxic T Cells | 2 x 1 mL | |
| K1204-10 | Monocytes | 2 x 1 mL | |
| K1205-10 | Leukocytes | 2 x 1 mL | |
| K1206-10 | B Cells | 2 x 1 mL | |
| K1207-10 | NK Cells | 2 x 2 mL |
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
RWD High Activity Enzymatic Digestion Kits
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
Optimized enzymatic hydrolysis formulation for efficient and gentle digestion. Preserves the integrity of surface antigenic epitopes. Store in refrigerators at 4°C or -20°C.
- Targeted optimization of enzymatic hydrolysis formula can ensure high cell viability.
- Preserving cell surface epitopes leads to no effect on downstream experiments.
Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.
For more information or alternative product recommendations, please contact us.
RWD Mouse and Microglia Cell Separation Kits
Mouse CD3+ Cell Separation Kit - K1301-10
For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.
Mouse CD4+ Cell Separation Kit - K1302-10
For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.
Mouse CD8+ Cell Separation Kit - K1303-10
For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.
Mouse CD45+ Cell Separation Kit - K1304-10
For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.
Mouse CD19+ Cell Separation Kit - K1305-10
For positive selection or depletion of B cells from mouse spleen and other samples.
CD11b+ (Microglia) cell separation Kit - K1306-10
For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.
Specifications
| Item # | Cell Type | Size | Capacity |
| K1301-10 | T Lymphocytes | 2 x 1 mL | For 1*10^9 Total Cells |
| K1302-10 | T Helper Cells | For 1*10^9 Total Cells | |
| K1303-10 | Cytotoxic T Cells | For 1*10^9 Total Cells | |
| K1304-10 | Leukocytes | For 1*10^9 Total Cells | |
| K1305-20 | B Cells | For 2*10^9 Total Cells | |
| K1306-10 | Myeloid Cells | For 1*10^9 Total Cells |
PrepGEM™ Universal DNA Extraction Kits
Single-tube, 15-minute DNA extraction solutions.
The PrepGEM™ Universal is a comprehensive DNA extraction kit designed for maximum yield, integrity, and ease of use. Engineered for researchers and technicians who demand efficiency and reliability, this adaptable reagent kit delivers high-quality DNA from both common and challenging sample types, all in a single tube.
Main Attributes
- Single tube simplifies processes, no transfers, no washes, no DNA loss
- Get rapid results from sample to DNA in ~15 minutes
- Broad compatibility optimized for blood, saliva, tissue, and cell culture
- Ideal for CRISPR genotyping and a wide range of downstream analyses
- Reduced plastic waste and hazardous chemicals for sustainable science
Simple and Effortless Extraction from Any Source
Consistently extract high-yield DNA from blood, saliva, tissues (including ear punches, mouse tails, and insects), and cell cultures. Our closed-tube, inhibitor-free method eliminates harsh chemical washes and multiple steps, ensuring no DNA is lost. This makes prepGEM™ Universal exceptionally powerful for low-cell-number extractions, down to a single cell.
Ideal for Advanced Genotyping and Sequencing
PrepGEM™ Universal is the perfect tool for performing confirmatory genotyping of transfected cells after CRISPR/Cas9 gene editing or preparing DNA for downstream applications. The kit produces ready-to-use DNA for STR analysis, PCR, qPCR, whole genome amplification, and amplicon sequencing.
Every Lab Deserves Rapid Results
Activate our proprietary thermophilic proteinase with a simple temperature change. The entire hands-off process is easily automated in a single tube using a standard thermocycler and takes approximately 15 minutes. Free up valuable technician time, reduce lab costs, and significantly cut down on plastic and hazardous waste.
Applications
- Blood Samples
- Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
- Saliva Samples
- Liquid, Buccal swab, Stains, Storage Card (FTA)
- Tissue Samples
- Tissue cultures, including single cell, Animal (scarpings, fat, muscle), Mouse and rat tail tips, Ear tags, Fish fins, Insects
Kit Components
- PrepGEM™
- Histosolv
- BLUE Buffer
- RED+ Buffer
- ORANGE+ Buffer
- PDQeX Kits Include PDQeX Kit Tubes (XPU0100, XPU0500, XPU1000)
PrepGEM™ Bacterial Enzymatic Kits
Speed and gentle precision for unparalleled insight into the microbial world.
The PrepGEM™ Bacteria enzymatic kit is designed for efficient, high-quality DNA extraction from a vast array of microbial sources. Engineered for microbiologists and metagenomics researchers, this method combines gentle lysis with a closed-tube workflow to preserve DNA integrity and ensure accurate community representation, all in a fraction of the time of traditional methods.
Main Attributes
- Enzymatic combination ensures gentle and efficient lysis while preserving DNA integrity
- Rapid results in a quarter of the time of conventional methods
- True Community Representation: Closed-tube, no-transfer process protects low-abundance species in metagenomic samples
- Broad compatibility from pure cultures to complex environmental samples
- Easily automated for high throughput in a secure, closed system for safety and scalability
Comprehensive Microbial and Metagenomic Profiling
Efficiently extract DNA from Gram-positive and Gram-negative bacteria, protozoa, archaea, colonies, and liquid cultures. The kit is equally effective for complex samples like biofilms, mucosal swabs, and environmental matrices, including soil, stool, and water. It is the ideal choice for metagenomic studies.
Integrity for Demanding Applications
The gentle, temperature-driven enzymatic lysis uses a unique combination of thermophilic proteinase and mesophilic lysozyme to minimize DNA fragmentation. With no mechanical disruption or transfer steps, you obtain exceptionally intact, high-molecular-weight DNA that accurately represents low-abundance species. The resulting DNA is ready for PCR, qPCR, targeted amplicon sequencing such as 16S rDNA analysis, and other downstream applications.
Accelerated Research Process, 1/4 the Time of Other Methods
The fully enclosed, single-tube system eliminates cross-contamination, protects users from pathogens, and requires minimal hands-on time. The automation-ready workflow reduces pipetting, preserves sample integrity, and drastically cuts down on plastic waste.
Applications
- Extraction from a variety of microbial samples including:
- Gram-positive and -negative bacteria, protozoa, colonies and liquid cultures, biofilm and mucosal, archaea, swabs, and metagenomic DNA: soil, stool, water
Kit Components
- PrepGEM™
- GREEN+ Buffer
- Lysozyme
- WASH+ Buffer
- Enhancer
- PDQeX Kits Include PDQeX Kit Tubes (XBA0100, XBA0500, XBA1000)
ForensicGEM™ Universal Enzymatic Kits
Precise, efficient, and secure solution for modern forensic DNA analysis.
ForensicGEM™ Universal is a DNA extraction kit designed for fast, reliable results in forensic science. Where speed, sample integrity, and minimal contamination are paramount, our innovative enzymatic process delivers analysis-ready DNA from challenging forensic samples in just 15 minutes.
Main Attributes
- Obtain STR, PCR, and qPCR-ready DNA in approximately 15 minutes
- Eliminating purification steps enables efficient extraction from very small sample volumes
- Single-tube, closed processing protects sample integrity and minimizes contamination risks
- Reduced hands-on time, lab costs, consumable use, and plastic waste
- Compatible with standard lab equipment for easy automation and adoption
Optimized for a Comprehensive Range of Forensic Evidence Types
Consistently extract high-yield DNA from saliva (liquid, swabs, stains, FTA cards), blood (liquid, swabs, stains, FTA cards), and tissue (solid samples and hair). The process is uniquely effective for both small-volume and trace samples.
Superior DNA for Demanding Forensic Analysis
The method produces DNA that is PCR-ready and suitable for STR profiling. Unlike traditional methods, our temperature-controlled process uses a novel cocktail of enzymes instead of harsh detergents and reducing agents like SDS or DTT. This eliminates common PCR inhibitors, ensuring superior results without purification steps.
Fully Hands-Off, Single-Tube Workflow
From sample to analysis, the process is contained, drastically reducing opportunities for contamination and protecting sample integrity. This approach is perfect for high-volume casework where quick turnaround is critical. The method is easily automated using existing thermal cyclers, freeing up valuable technician time and accelerating response times.
For extracting DNA from sperm, use ForensicGEM™ Sperm.
Applications
- Extraction from a variety of forensic blood samples
- Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
- Tissue samples
- Animal (scrapings, fat, and muscle), Tape lifts, Hair
- Saliva samples
- Liquid, Swab, Stains, Storage Card (FTA)
ForensicGEM™ is a preparative method for DNA extraction. The method lyses cells and removes nucleoproteins from the DNA. Extracted DNA can be used for many types of genotyping, including SNP and STR analysis as well as quantitative, multiplex, and endpoint PCR.
There is no concentration step in the procedure, so the concentration of the extract is dependent on:
- The quality of the sample
- In the case of swabs, the type of swab and the volume of water used to wash the swab
- The extraction volume (which in some cases can be scaled)
DNA extracted using ForensicGEM™ is largely single stranded because of the 95ºC heat step. For accurate yield assessment, a qPCR is recommended. If standard fluorescent chelating dyes are to be used for normalising samples, then we recommend taking a sample of the extract before the 95ºC step. Alternatively, you can generate a standard curve using a previously made extract that has been quantified.
Kit Components
- ForensicGEM™
- BLUE Buffer
- RED+ Buffer
- ORANGE+ Buffer
- Histosolv
- PDQeX Kits Include PDQeX Kit Tubes (XFU0100, XFU0500, XFU1000)
ForensicGEM™ Sperm Enzymatic Kits
Precision and efficiency for forensic differential extraction.
Make the complex challenge of sperm cell lysis fast and simple. ForensicGEM™ Sperm is a specialized enzymatic kit designed for the rapid, single-tube extraction of high-quality DNA from sperm cells, semen samples, and vaginal swabs. Eliminating the need for harsh, inhibitory chemicals and lengthy purification helps achieve superior yields and STR-ready DNA in just 11 minutes.
Main Attributes
- Ultra-fast results, analysis-ready DNA in ~11 minutes.
- No purification or bead/column steps means no DNA loss, enabling work with very small cell numbers
- Inhibitor-free DNA: eliminates harsh chemicals like DTT that interfere with STR and qPCR, removing the need for post-lysis cleanup
- Single-tube, no-transfer processing safeguards results and minimizes the risk of contamination
- Frees technician time, reduces plasticware use, cuts costs, and is easily automated
Reliable DNA Extraction from Sperm Cells
Sperm cells are notoriously difficult to lyse without compromising downstream analysis. This kit enables efficient differential extraction workflows and Y-screening, even from samples with very low sperm counts. The method produces high-quality double-stranded DNA for STR profiling, PCR, and qPCR.
Free of Downstream Inhibitory Compounds/Chemicals
Traditional methods rely on STR-inhibiting chemicals like DTT, SDS, and mercaptoethanol, which are toxic to downstream processes and require time-consuming purification steps that cause DNA loss. ForensicGEM™ Sperm uses a novel, temperature-controlled cocktail of thermophilic and mesophilic enzymes.
~11-Minute, Hands-Off process Completed in a Single Tube
This closed-tube system eliminates transfer steps, minimizing opportunities for contamination, cross-talk, and human error while protecting sample integrity. The protocol is compatible with existing thermal cyclers and easily automated for high-throughput processing, from low-volume casework to large-scale batches. This rapid processing means sex crime cases can be triaged very quickly to determine if a Y chromosome is present in the sample.
Applications
- Extraction from semen, including:
- Swab, Stain, Liquid
Kit Components
- ForensicGEM™
- ORANGE+ Buffer
- Acrosolv
- PDQeX Kits Include PDQeX Kit Tubes (XFS0100, XFS0500, XFS1000)
PhytoGEM™ Enzymatic Kits
Robust, automated solution for modern plant science and mycology.
Eliminate the challenges of plant and fungal DNA extraction with phytoGEM™, the specialized kit designed for the automated, high-quality isolation of DNA from even the toughest samples. From resilient leaf tissue to complex fungal pathogens, our enzymatic lysis method overcomes inhibitory compounds and tough cell walls, delivering PCR and qPCR-ready DNA without laborious grinding, columns, or hazardous solvents.
Main Attributes
- Enzymatic lysis efficiently breaks down tough cells, handling plant and fungal cell walls without mechanical grinding
- Produces clean DNA free from polysaccharides and polyphenols that inhibit PCR
- Enables simple, closed-tube processing on the PDQeX system for high consistency and safety
- Accelerates pathogen detection and genetic analysis from hours to minutes for rapid results
- Trusted for key plant diseases and fungal genomics
Designed for Diverse Botanical and Mycological Samples
Effortlessly extract DNA from a wide variety of plant materials, including leaf, stem, and root tissues, as well as from plant pathogens, fungi, and oomycetes. PhytoGEM™ is the ideal tool for studying plant genotypes, genetic variation, endophytic pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and verifying the authenticity of botanical extracts.
Overcomes Traditional Extraction Challenges
Conventional methods struggle with the polysaccharides, tannins, and polyphenols released from plant and fungal cells, requiring multi-step, time-intensive processes that often degrade DNA. PhytoGEM’s proprietary, temperature-controlled enzymatic lysis effectively breaks down tough cell walls in a closed-tube system. This gentle approach eliminates harsh chemical washes, preserves DNA integrity, and yields inhibitor-free DNA suitable for sensitive downstream applications.
Proactive Disease Control Through Rapid Detection of Critical Pathogens
Early detection is critical for crop management. PhytoGEM™, used with the automated PDQeX Nucleic Acid Extractor, transforms this bottleneck into an easy process. From a simple leaf punch on a storage card or homogenized tissue, the system performs precise, closed-tube lysis and purification, drastically reducing hands-on time and contamination risk.
Validated for Key Agricultural Threats
- The PhytoGEM™ method has been specifically validated for detecting a range of economically significant pathogens, including:
- Bacteria: Xylella fastidiosa, Pseudomonas syringae (Psa), Candidatus Liberibacter solanacearum
- Oomycetes: Phytophthora species
- Phytoplasma: In grapevines
- Viruses: Cassava Mosaic Virus
- Fungi: Providing high-quality DNA for genomics, strain characterization, and environmental studies
Applications
- Plant samples
- Leaf, Stem, Root, Plant Pathogens, Fungi
Kit Components
- PhytoGEM™
- Histosolv
- GREEN+ Buffer
- Enhancer
- PDQeX Kits Include PDQeX Kit Tubes (XPP0100, XPP0500, XPP1000)
RNAGEM™ Nucleic Acid Extraction Kits
Effortless, ultra-fast nucleic acid extraction.
RNAGEM™ utilizes Exymes' novel thermophilic proteinase and buffer system to extract total nucleic acids in less than 25 minutes without the need for purification.
- Sample types include cell culture, single-cell, and exosomes
- Downstream applications include PCR, RT-PCR, RT-qPCR, LAMP, RT-LAMP, and Nanostring
Main Attributes
- Single-tube nucleic acid extraction in less than 25 minutes
- Total nucleic acid extraction
- genomic DNA
- mRNA
- long non-coding RNA
- small non-coding RNA
- No ionic detergents or chaotropic salts
- High nucleic acid recovery, minimal loss of nucleic acids during extraction
- No magnetic beads, no spin-columns
- Suitable for low-throughput to high-throughput extraction with a single protocol
- Easily automated using standard liquid handling solutions
- Minimal plasticware required, reduces waste and supply chain issues
Applications
- Total RNA extraction from
- Mammalian cells, Virus
Kit Components
- RNAGEM™
- BLUE Buffer
- DNase I
- Dnase Buffer
- TE Buffer
Lab-Sorb™ Granules
Converts liquid waste to solid waste in seconds. LabSorb™ rapidly absorbs aqueous or mixed aqueous/organic solvent waste. Use at locations connected to septic systems to avoid pouring waste down the drain. Put LabSorb™ at the bottom of your biohazard bin. One jar of LabSorb™ can absorb up to 50 liters of liquid. Retains liquid during autoclaving.
Includes extra durable disposal bags, super absorbent LabSorb™ granules, and a convenient graduated scoop.
- Lab-Sorb™ Kits: SORB-2000 (Small Jar), SORB-2100 (Medium Jar), and SORB-2200 (Large Jar) Contain 1 jar of LabSorb™, 25 small disposal bags, 25 twist-ties
- Variety Kit Contents: 1 Small LabSorb™ Jar, 15 small disposal bags, 10 medium disposal bags, 5 large disposal bags, 25 twist-ties
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