Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.   

RWD Mouse and Microglia Cell Separation Kits

Mouse CD3+ Cell Separation Kit - K1301-10

For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.

Mouse CD4+ Cell Separation Kit - K1302-10

For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.

Mouse CD8+ Cell Separation Kit - K1303-10

For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.

Mouse CD45+ Cell Separation Kit - K1304-10

For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.

Mouse CD19+ Cell Separation Kit - K1305-10

For positive selection or depletion of B cells from mouse spleen and other samples.

CD11b+ (Microglia) cell separation Kit - K1306-10

For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.

Specifications
 

Item #	Cell Type	Size	Capacity
K1301-10	T Lymphocytes	2 x 1 mL	For 1*10^9 Total Cells
K1302-10	T Helper Cells		For 1*10^9 Total Cells
K1303-10	Cytotoxic T Cells		For 1*10^9 Total Cells
K1304-10	Leukocytes		For 1*10^9 Total Cells
K1305-20	B Cells		For 2*10^9 Total Cells
K1306-10	Myeloid Cells		For 1*10^9 Total Cells

 

PrepGEM™ Universal DNA Extraction Kits

Single-tube, 15-minute DNA extraction solutions.

The PrepGEM™ Universal is a comprehensive DNA extraction kit designed for maximum yield, integrity, and ease of use. Engineered for researchers and technicians who demand efficiency and reliability, this adaptable reagent kit delivers high-quality DNA from both common and challenging sample types, all in a single tube.

Main Attributes

• Single tube simplifies processes, no transfers, no washes, no DNA loss
• Get rapid results from sample to DNA in ~15 minutes
• Broad compatibility optimized for blood, saliva, tissue, and cell culture
• Ideal for CRISPR genotyping and a wide range of downstream analyses
• Reduced plastic waste and hazardous chemicals for sustainable science

Simple and Effortless Extraction from Any Source

Consistently extract high-yield DNA from blood, saliva, tissues (including ear punches, mouse tails, and insects), and cell cultures. Our closed-tube, inhibitor-free method eliminates harsh chemical washes and multiple steps, ensuring no DNA is lost. This makes prepGEM™ Universal exceptionally powerful for low-cell-number extractions, down to a single cell.

Ideal for Advanced Genotyping and Sequencing

PrepGEM™ Universal is the perfect tool for performing confirmatory genotyping of transfected cells after CRISPR/Cas9 gene editing or preparing DNA for downstream applications. The kit produces ready-to-use DNA for STR analysis, PCR, qPCR, whole genome amplification, and amplicon sequencing.

Every Lab Deserves Rapid Results

Activate our proprietary thermophilic proteinase with a simple temperature change. The entire hands-off process is easily automated in a single tube using a standard thermocycler and takes approximately 15 minutes. Free up valuable technician time, reduce lab costs, and significantly cut down on plastic and hazardous waste.

Applications

• Blood Samples
• Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
• Saliva Samples
• Liquid, Buccal swab, Stains, Storage Card (FTA)
• Tissue Samples
• Tissue cultures, including single cell, Animal (scarpings, fat, muscle), Mouse and rat tail tips, Ear tags, Fish fins, Insects 

Kit Components

• PrepGEM™
• Histosolv
• BLUE Buffer
• RED+ Buffer
• ORANGE+ Buffer
• PDQeX Kits Include PDQeX Kit Tubes (XPU0100, XPU0500, XPU1000)

PrepGEM™ Bacterial Enzymatic Kits

Speed and gentle precision for unparalleled insight into the microbial world.

The PrepGEM™ Bacteria enzymatic kit is designed for efficient, high-quality DNA extraction from a vast array of microbial sources. Engineered for microbiologists and metagenomics researchers, this method combines gentle lysis with a closed-tube workflow to preserve DNA integrity and ensure accurate community representation, all in a fraction of the time of traditional methods.

Main Attributes

• Enzymatic combination ensures gentle and efficient lysis while preserving DNA integrity
• Rapid results in a quarter of the time of conventional methods
• True Community Representation: Closed-tube, no-transfer process protects low-abundance species in metagenomic samples
• Broad compatibility from pure cultures to complex environmental samples
• Easily automated for high throughput in a secure, closed system for safety and scalability 

Comprehensive Microbial and Metagenomic Profiling

Efficiently extract DNA from Gram-positive and Gram-negative bacteria, protozoa, archaea, colonies, and liquid cultures. The kit is equally effective for complex samples like biofilms, mucosal swabs, and environmental matrices, including soil, stool, and water. It is the ideal choice for metagenomic studies.

Integrity for Demanding Applications

The gentle, temperature-driven enzymatic lysis uses a unique combination of thermophilic proteinase and mesophilic lysozyme to minimize DNA fragmentation. With no mechanical disruption or transfer steps, you obtain exceptionally intact, high-molecular-weight DNA that accurately represents low-abundance species. The resulting DNA is ready for PCR, qPCR, targeted amplicon sequencing such as 16S rDNA analysis, and other downstream applications.

Accelerated Research Process, 1/4 the Time of Other Methods

The fully enclosed, single-tube system eliminates cross-contamination, protects users from pathogens, and requires minimal hands-on time. The automation-ready workflow reduces pipetting, preserves sample integrity, and drastically cuts down on plastic waste.
 

Applications

• Extraction from a variety of microbial samples including:
• Gram-positive and -negative bacteria, protozoa, colonies and liquid cultures, biofilm and mucosal, archaea, swabs, and metagenomic DNA: soil, stool, water 

Kit Components

• PrepGEM™
• GREEN+ Buffer
• Lysozyme
• WASH+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XBA0100, XBA0500, XBA1000)

ForensicGEM™ Universal Enzymatic Kits

Precise, efficient, and secure solution for modern forensic DNA analysis.

ForensicGEM™ Universal is a DNA extraction kit designed for fast, reliable results in forensic science. Where speed, sample integrity, and minimal contamination are paramount, our innovative enzymatic process delivers analysis-ready DNA from challenging forensic samples in just 15 minutes.

Main Attributes

• Obtain STR, PCR, and qPCR-ready DNA in approximately 15 minutes
• Eliminating purification steps enables efficient extraction from very small sample volumes
• Single-tube, closed processing protects sample integrity and minimizes contamination risks
• Reduced hands-on time, lab costs, consumable use, and plastic waste
• Compatible with standard lab equipment for easy automation and adoption 

Optimized for a Comprehensive Range of Forensic Evidence Types

Consistently extract high-yield DNA from saliva (liquid, swabs, stains, FTA cards), blood (liquid, swabs, stains, FTA cards), and tissue (solid samples and hair). The process is uniquely effective for both small-volume and trace samples.

Superior DNA for Demanding Forensic Analysis

The method produces DNA that is PCR-ready and suitable for STR profiling. Unlike traditional methods, our temperature-controlled process uses a novel cocktail of enzymes instead of harsh detergents and reducing agents like SDS or DTT. This eliminates common PCR inhibitors, ensuring superior results without purification steps.

Fully Hands-Off, Single-Tube Workflow

From sample to analysis, the process is contained, drastically reducing opportunities for contamination and protecting sample integrity. This approach is perfect for high-volume casework where quick turnaround is critical. The method is easily automated using existing thermal cyclers, freeing up valuable technician time and accelerating response times.

For extracting DNA from sperm, use ForensicGEM™ Sperm.
 

Applications

• Extraction from a variety of forensic blood samples
• Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
• Tissue samples
• Animal (scrapings, fat, and muscle), Tape lifts, Hair
• Saliva samples
• Liquid, Swab, Stains, Storage Card (FTA) 

ForensicGEM™ is a preparative method for DNA extraction. The method lyses cells and removes nucleoproteins from the DNA. Extracted DNA can be used for many types of genotyping, including SNP and STR analysis as well as quantitative, multiplex, and endpoint PCR.

There is no concentration step in the procedure, so the concentration of the extract is dependent on:

• The quality of the sample
• In the case of swabs, the type of swab and the volume of water used to wash the swab
• The extraction volume (which in some cases can be scaled)

DNA extracted using ForensicGEM™ is largely single stranded because of the 95ºC heat step. For accurate yield assessment, a qPCR is recommended. If standard fluorescent chelating dyes are to be used for normalising samples, then we recommend taking a sample of the extract before the 95ºC step. Alternatively, you can generate a standard curve using a previously made extract that has been quantified.

Kit Components

• ForensicGEM™
• BLUE Buffer
• RED+ Buffer
• ORANGE+ Buffer
• Histosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFU0100, XFU0500, XFU1000)

ForensicGEM™ Sperm Enzymatic Kits

Precision and efficiency for forensic differential extraction.

Make the complex challenge of sperm cell lysis fast and simple. ForensicGEM™ Sperm is a specialized enzymatic kit designed for the rapid, single-tube extraction of high-quality DNA from sperm cells, semen samples, and vaginal swabs. Eliminating the need for harsh, inhibitory chemicals and lengthy purification helps achieve superior yields and STR-ready DNA in just 11 minutes.

Main Attributes

• Ultra-fast results, analysis-ready DNA in ~11 minutes.
• No purification or bead/column steps means no DNA loss, enabling work with very small cell numbers
• Inhibitor-free DNA: eliminates harsh chemicals like DTT that interfere with STR and qPCR, removing the need for post-lysis cleanup
• Single-tube, no-transfer processing safeguards results and minimizes the risk of contamination
• Frees technician time, reduces plasticware use, cuts costs, and is easily automated 

Reliable DNA Extraction from Sperm Cells

Sperm cells are notoriously difficult to lyse without compromising downstream analysis. This kit enables efficient differential extraction workflows and Y-screening, even from samples with very low sperm counts. The method produces high-quality double-stranded DNA for STR profiling, PCR, and qPCR.

Free of Downstream Inhibitory Compounds/Chemicals

Traditional methods rely on STR-inhibiting chemicals like DTT, SDS, and mercaptoethanol, which are toxic to downstream processes and require time-consuming purification steps that cause DNA loss. ForensicGEM™ Sperm uses a novel, temperature-controlled cocktail of thermophilic and mesophilic enzymes.

~11-Minute, Hands-Off process Completed in a Single Tube

This closed-tube system eliminates transfer steps, minimizing opportunities for contamination, cross-talk, and human error while protecting sample integrity. The protocol is compatible with existing thermal cyclers and easily automated for high-throughput processing, from low-volume casework to large-scale batches. This rapid processing means sex crime cases can be triaged very quickly to determine if a Y chromosome is present in the sample.

Applications

• Extraction from semen, including:
• Swab, Stain, Liquid 

Kit Components

• ForensicGEM™
• ORANGE+ Buffer
• Acrosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFS0100, XFS0500, XFS1000)

PhytoGEM™ Enzymatic Kits

Robust, automated solution for modern plant science and mycology.

Eliminate the challenges of plant and fungal DNA extraction with phytoGEM™, the specialized kit designed for the automated, high-quality isolation of DNA from even the toughest samples. From resilient leaf tissue to complex fungal pathogens, our enzymatic lysis method overcomes inhibitory compounds and tough cell walls, delivering PCR and qPCR-ready DNA without laborious grinding, columns, or hazardous solvents.

Main Attributes

• Enzymatic lysis efficiently breaks down tough cells, handling plant and fungal cell walls without mechanical grinding
• Produces clean DNA free from polysaccharides and polyphenols that inhibit PCR
• Enables simple, closed-tube processing on the PDQeX system for high consistency and safety
• Accelerates pathogen detection and genetic analysis from hours to minutes for rapid results
• Trusted for key plant diseases and fungal genomics 

Designed for Diverse Botanical and Mycological Samples

Effortlessly extract DNA from a wide variety of plant materials, including leaf, stem, and root tissues, as well as from plant pathogens, fungi, and oomycetes. PhytoGEM™ is the ideal tool for studying plant genotypes, genetic variation, endophytic pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and verifying the authenticity of botanical extracts.

Overcomes Traditional Extraction Challenges

Conventional methods struggle with the polysaccharides, tannins, and polyphenols released from plant and fungal cells, requiring multi-step, time-intensive processes that often degrade DNA. PhytoGEM’s proprietary, temperature-controlled enzymatic lysis effectively breaks down tough cell walls in a closed-tube system. This gentle approach eliminates harsh chemical washes, preserves DNA integrity, and yields inhibitor-free DNA suitable for sensitive downstream applications.

Proactive Disease Control Through Rapid Detection of Critical Pathogens

Early detection is critical for crop management. PhytoGEM™, used with the automated PDQeX Nucleic Acid Extractor, transforms this bottleneck into an easy process. From a simple leaf punch on a storage card or homogenized tissue, the system performs precise, closed-tube lysis and purification, drastically reducing hands-on time and contamination risk.

Validated for Key Agricultural Threats

• The PhytoGEM™ method has been specifically validated for detecting a range of economically significant pathogens, including:
• Bacteria: Xylella fastidiosa, Pseudomonas syringae (Psa), Candidatus Liberibacter solanacearum
• Oomycetes: Phytophthora species
• Phytoplasma: In grapevines
• Viruses: Cassava Mosaic Virus
• Fungi: Providing high-quality DNA for genomics, strain characterization, and environmental studies 

Applications

• Plant samples
• Leaf, Stem, Root, Plant Pathogens, Fungi 

Kit Components

• PhytoGEM™
• Histosolv
• GREEN+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XPP0100, XPP0500, XPP1000)

RNAGEM™ Nucleic Acid Extraction Kits

Effortless, ultra-fast nucleic acid extraction.

RNAGEM™ utilizes Exymes' novel thermophilic proteinase and buffer system to extract total nucleic acids in less than 25 minutes without the need for purification.

• Sample types include cell culture, single-cell, and exosomes
• Downstream applications include PCR, RT-PCR, RT-qPCR, LAMP, RT-LAMP, and Nanostring 

Main Attributes

• Single-tube nucleic acid extraction in less than 25 minutes
• Total nucleic acid extraction
• genomic DNA
• mRNA
• long non-coding RNA
• small non-coding RNA
• No ionic detergents or chaotropic salts
• High nucleic acid recovery, minimal loss of nucleic acids during extraction
• No magnetic beads, no spin-columns
• Suitable for low-throughput to high-throughput extraction with a single protocol
• Easily automated using standard liquid handling solutions
• Minimal plasticware required, reduces waste and supply chain issues 

Applications

• Total RNA extraction from
• Mammalian cells, Virus 

Kit Components

• RNAGEM™
• BLUE Buffer
• DNase I
• Dnase Buffer
• TE Buffer

Lab-Sorb™ Granules

Converts liquid waste to solid waste in seconds. LabSorb™ rapidly absorbs aqueous or mixed aqueous/organic solvent waste. Use at locations connected to septic systems to avoid pouring waste down the drain. Put LabSorb™ at the bottom of your biohazard bin. One jar of LabSorb™ can absorb up to 50 liters of liquid. Retains liquid during autoclaving.

Includes extra durable disposal bags, super absorbent LabSorb™ granules, and a convenient graduated scoop.

• Lab-Sorb™ Kits: SORB-2000 (Small Jar), SORB-2100 (Medium Jar), and SORB-2200 (Large Jar) Contain 1 jar of LabSorb™, 25 small disposal bags, 25 twist-ties
• Variety Kit Contents: 1 Small LabSorb™ Jar, 15 small disposal bags, 10 medium disposal bags, 5 large disposal bags, 25 twist-ties   

Proudly made in the USA! Click here for more made in America products!

PR1MA™ Agarose 500 mg Tablets

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Save time and labor preparing agarose gels for electrophoresis of nucleic acids. Just place the required number of tablets into a standard buffer, then mix and heat until completely dissolved.

Tablet Weight Range: 485-515 mg
Gel Strength, 1.5%: >1200 g/cm2
Gelling Range: 36-39°C
Melting Temp: 87-89°C
EEO: <0.10
Sulfate: <0.15
DNase, RNase: None Detected

Research or further manufacturing use only, not for food or drug use.

• Appearance: White tablets
• Gelling Range (1.5% Water): 36 - 39 ^oC
• pH (3% water, 25°C): 5.7 - 7.7
• Tablet Weight: 485 - 515 mg

These items have been discontinued and will only be available while supplies last.

Looking for alternatives? Contact us or see our other Agarose options!

Bullseye Agarose General Purpose

Bullseye Molecular Biology Grade Agarose is certified for use in standard electrophoresis procedures, including nucleic acid analysis, preparative electrophoresis, and blotting. This highly refined and purified agarose is produced under strict testing parameters to ensure lot-to-lot consistency. It features low electrophoretic mobility characteristics, making it ideal for a variety of applications.

Extracted from the cell walls of certain Rhodophyceae (agarophyte seaweeds), Bullseye Standard Agarose is a neutral polysaccharide composed of galactan, linked by agarobioses in a 1-3, 1-4 arrangement. This unique chemical structure enables agarose to form robust gels even at low concentrations. The gels exhibit a macroreticular structure with an open mesh, easily adjustable by varying the agarose concentration.

Bullseye Agarose GP2 offers

• General-purpose electrophoresis and blotting capabilities
• Excellent separation, resolution, and clarity
• Low absorption and background from staining reagents
• High gel strength for safe and easy handling
• Superior refinement and purification
• Enhanced thermal stability
• Comprehensive packaging including a specification sheet, preparation instructions, and MSDS
• Choose Bullseye Standard Agarose for reliable performance in your molecular biology applications. 

Physical Specifications

• CAS#: 9012-36-6
• Moisture: ≤ 7%
• Ash: ≤ 0.4%
• EEO: 0.05-0.13
• Sulfate: ≤ 0.10%
• Clarity @ 1.5% (NTU): ≤ 3
• Gel Strength @ 1.0% (g/cm2): ≥ 1200
• Gel Strength @ 1.5% (g/cm2): ≥ 2500
• Gelling Temperature @ 1.5%: 36°C ± 1.5°C
• Melting Temperature @ 1.5%: 88°C ± 1.5°C 

Molecular Biology Specifications

• DNase assay: None Detected
• RNase assay: None Detected
• Protease assay: None Detected
• Gel analysis assay: Pass
• DNA Resolution: ≥ 1000bp (Finely Resolved)
• DNA Extraction assay: Pass
• Gel Background: Very Low

This brand is being discontinued and will only be available while supplies last.

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.

Bullseye DNA SafeStain,10,000x 

• Safest DNA stain by far
• Replaces EthBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Excitation at 290 nm & 490 nm
• Emission at 530 nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

*For the proper disposal of this product, follow University or Company Guidelines.

Bullseye RT 2X Master Mix

• Up to 9kb cDNA synthesis
• Ensures sample to sample consistency
• Large RNA sample volume capacity
• Ready to use

Size: 100 rxns

RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.

Kit Components

Storage

• Store at -20°C in a frost-free freezer.

Application

• cDNA synthesis
• Construction of cDNA libraries
• Generation of probes for hybridization 

Protocol

1. Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
2. Assemble the following components in a tube on ice, and mix well:

1. Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
2. Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
3. Mix well and collect all the components by a brief centrifugation.
4. Incubate the tube at room temperature for 10 min for annealing.
5. Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
6. Stop the reaction by heating it at 85°C for 5 min.
7. Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications.