ForensicGEM™ Universal Enzymatic Kits

Precise, efficient, and secure solution for modern forensic DNA analysis.

ForensicGEM™ Universal is a DNA extraction kit designed for fast, reliable results in forensic science. Where speed, sample integrity, and minimal contamination are paramount, our innovative enzymatic process delivers analysis-ready DNA from challenging forensic samples in just 15 minutes.

Main Attributes

• Obtain STR, PCR, and qPCR-ready DNA in approximately 15 minutes
• Eliminating purification steps enables efficient extraction from very small sample volumes
• Single-tube, closed processing protects sample integrity and minimizes contamination risks
• Reduced hands-on time, lab costs, consumable use, and plastic waste
• Compatible with standard lab equipment for easy automation and adoption 

Optimized for a Comprehensive Range of Forensic Evidence Types

Consistently extract high-yield DNA from saliva (liquid, swabs, stains, FTA cards), blood (liquid, swabs, stains, FTA cards), and tissue (solid samples and hair). The process is uniquely effective for both small-volume and trace samples.

Superior DNA for Demanding Forensic Analysis

The method produces DNA that is PCR-ready and suitable for STR profiling. Unlike traditional methods, our temperature-controlled process uses a novel cocktail of enzymes instead of harsh detergents and reducing agents like SDS or DTT. This eliminates common PCR inhibitors, ensuring superior results without purification steps.

Fully Hands-Off, Single-Tube Workflow

From sample to analysis, the process is contained, drastically reducing opportunities for contamination and protecting sample integrity. This approach is perfect for high-volume casework where quick turnaround is critical. The method is easily automated using existing thermal cyclers, freeing up valuable technician time and accelerating response times.

For extracting DNA from sperm, use ForensicGEM™ Sperm.
 

Applications

• Extraction from a variety of forensic blood samples
• Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
• Tissue samples
• Animal (scrapings, fat, and muscle), Tape lifts, Hair
• Saliva samples
• Liquid, Swab, Stains, Storage Card (FTA) 

ForensicGEM™ is a preparative method for DNA extraction. The method lyses cells and removes nucleoproteins from the DNA. Extracted DNA can be used for many types of genotyping, including SNP and STR analysis as well as quantitative, multiplex, and endpoint PCR.

There is no concentration step in the procedure, so the concentration of the extract is dependent on:

• The quality of the sample
• In the case of swabs, the type of swab and the volume of water used to wash the swab
• The extraction volume (which in some cases can be scaled)

DNA extracted using ForensicGEM™ is largely single stranded because of the 95ºC heat step. For accurate yield assessment, a qPCR is recommended. If standard fluorescent chelating dyes are to be used for normalising samples, then we recommend taking a sample of the extract before the 95ºC step. Alternatively, you can generate a standard curve using a previously made extract that has been quantified.

Kit Components

• ForensicGEM™
• BLUE Buffer
• RED+ Buffer
• ORANGE+ Buffer
• Histosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFU0100, XFU0500, XFU1000)