PrepGEM™ Bacterial Enzymatic Kits

Speed and gentle precision for unparalleled insight into the microbial world.

The PrepGEM™ Bacteria enzymatic kit is designed for efficient, high-quality DNA extraction from a vast array of microbial sources. Engineered for microbiologists and metagenomics researchers, this method combines gentle lysis with a closed-tube workflow to preserve DNA integrity and ensure accurate community representation, all in a fraction of the time of traditional methods.

Main Attributes

• Enzymatic combination ensures gentle and efficient lysis while preserving DNA integrity
• Rapid results in a quarter of the time of conventional methods
• True Community Representation: Closed-tube, no-transfer process protects low-abundance species in metagenomic samples
• Broad compatibility from pure cultures to complex environmental samples
• Easily automated for high throughput in a secure, closed system for safety and scalability 

Comprehensive Microbial and Metagenomic Profiling

Efficiently extract DNA from Gram-positive and Gram-negative bacteria, protozoa, archaea, colonies, and liquid cultures. The kit is equally effective for complex samples like biofilms, mucosal swabs, and environmental matrices, including soil, stool, and water. It is the ideal choice for metagenomic studies.

Integrity for Demanding Applications

The gentle, temperature-driven enzymatic lysis uses a unique combination of thermophilic proteinase and mesophilic lysozyme to minimize DNA fragmentation. With no mechanical disruption or transfer steps, you obtain exceptionally intact, high-molecular-weight DNA that accurately represents low-abundance species. The resulting DNA is ready for PCR, qPCR, targeted amplicon sequencing such as 16S rDNA analysis, and other downstream applications.

Accelerated Research Process, 1/4 the Time of Other Methods

The fully enclosed, single-tube system eliminates cross-contamination, protects users from pathogens, and requires minimal hands-on time. The automation-ready workflow reduces pipetting, preserves sample integrity, and drastically cuts down on plastic waste.
 

Applications

• Extraction from a variety of microbial samples including:
• Gram-positive and -negative bacteria, protozoa, colonies and liquid cultures, biofilm and mucosal, archaea, swabs, and metagenomic DNA: soil, stool, water 

Kit Components

• PrepGEM™
• GREEN+ Buffer
• Lysozyme
• WASH+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XBA0100, XBA0500, XBA1000)