Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features
 

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

2026 On Target Promo!

 

For a limited time, buy one Accuris™ Fast Extraction PCR Kit,
High Fidelity Hot Start Red Dye Master Mix, 100 Reactions
(PR1400-FE-100), and get ONE FREE (PR1400-FE-100-VF).

  

For free product, add item PR1400-FE-100 at $127.53

PLUS (1) item PR1400-FE-100-VF to your cart.

 

Use promo code: OT202

 

Click here for more On Target savings

or see flyer for more details.

 

Promotion valid until 10/31/26 or while supplies last, for eligible U.S. customers.

Prices are in U.S. dollars and exclude freight, taxes, installation, and other applicable charges.

Offers may not be combined with other offers, discounts, or institutional pricing.

Customer must pay the list price on the qualifying item to receive the free item (List Price is reflected in flyer).

Buy-one-get-one offers apply to identical eligible catalog numbers unless otherwise stated.

All terms and conditions apply.

Accuris™ Fast Extraction PCR Kit

The Accuris™ Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

• Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

Proudly made in the USA! Click here for more made in America products!

PR1MA™ Agar, High Gel Strength, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Purified high gel strength agar with excellent properties for tissue culture and microbiological work.

Molecular Biology Grade

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Cream Powder
• Ash: ≤ 4.5 %
• Gel Point (1.5% Solution): 33 - 38 °C
• Gel Strength (1.5% Gel): ≥ 800 g/cm²
• Loss on Drying: ≤ 12.0 %
• Melting Point (1.5 %): 88.0 - 96.0
• pH (1.5% solution): 6.0 - 8.0

PR1MA™ Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Purified agar with excellent properties for tissue culture and microbiological work.

• To prepare media, add 15 grams to 1 liter of water.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Cream Color, Average Particle Size Between 1-2 mm
• Granulometry (ASTM) 60 Mesh Sieve Size: Report %
• Ash: ≤ 6.5 %
• E.Coli: Negative
• Gel Point (1.5% Gel): 33 - 38°C
• Gel Strength (1.5% Gel): 600 - 1100 g/cm²
• Melting Point: 88 - 96°C
• Moisture : ≤ 15 %
• Molds and Yeasts: < 100 cfu/g
• pH: 6.0 - 8.0
• Total Plate Count: < 1000 cfu/g
• Viscosity (1.5 Solution @ 60 ^oC): ≤ 30 cps
• Water Absorption: ≤ 75 cc

PR1MA™  Agarose, Low EEO

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Molecular Biology Grade. High purity for preparative and analytical electrophoresis of nucleic acids >1000 bp.

Gelling Temperature: 34.5 -37.5°C
Sulfate: 0.15
EEO: 0.09- 0.13
DNA Binding: None Detected
DNA and RNase: None Detected

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• DNase/ RNase: Not Detected
• EEO: 0.09 - 0.13
• Gelling Point: 34.5 - 37.5 °C
• Gel Strength (1.0%): ≥ 1200 g/cm²
• Moisture Content: ≤ 10.0 %
• Sulfate: ≤ 0.15 %

PR1MA™ Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.

Gel Strength, 4%: ≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Powder
• EEO: ≤ 0.13 -Mr
• Gelling Temperature: 32.5 - 38.0 ^oC
• Gel Strength : ≥ 1400 g/cm²
• Melting Temperature: ≤ 90.0 ^oC
• Moisture Content: ≤ 10.0 %
• Sulfate: ≤ 0.15 %

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.

Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to off white powder
• DNase/ RNase: Not Detected
• EEO: ≤ 0.10 -Mr
• Gelling Temperature: 26 - 30 ^oC
• Gel Strength : ≥ 200 g/cm²
• Melting Temperature: ≤ 65 ^oC
• Moisture Content: ≤ 10 %
• Sulfate Content: ≤ 0.10 %

Vion Ultrapure ASTM Type I Water

Our ultrapure, ASTM Type I, water meets the strictest standards defined by the American Society for Testing and Materials (ASTM). It is ideal for a wide range of ultra-sensitive laboratory uses include gas chromatography, HPLC, mass spectrometry, atomic absorption spectroscopy, and trace analysis.

Specifications
 

Resistivity	≥ 18 MΩ
Conductivity	≤ 0.056 uS / cm
Total Organic Carbon (TOC)	≤ 50 ppb
Silicate (as SiO2)	≤ 3 ppb
Chloride (Cl)	≤ 1 ppb
Sodium (Na)	≤ 1 ppb
Endotoxin	≤ 0.03 EU / mL
Heterotrophic BacteriaCount (HBC)	≤ 10 CFU / L

 

Proudly made in the USA! Click here for more made in America products!