PhytoGEM™ Enzymatic Kits

Robust, automated solution for modern plant science and mycology.

Eliminate the challenges of plant and fungal DNA extraction with phytoGEM™, the specialized kit designed for the automated, high-quality isolation of DNA from even the toughest samples. From resilient leaf tissue to complex fungal pathogens, our enzymatic lysis method overcomes inhibitory compounds and tough cell walls, delivering PCR and qPCR-ready DNA without laborious grinding, columns, or hazardous solvents.

Main Attributes

• Enzymatic lysis efficiently breaks down tough cells, handling plant and fungal cell walls without mechanical grinding
• Produces clean DNA free from polysaccharides and polyphenols that inhibit PCR
• Enables simple, closed-tube processing on the PDQeX system for high consistency and safety
• Accelerates pathogen detection and genetic analysis from hours to minutes for rapid results
• Trusted for key plant diseases and fungal genomics 

Designed for Diverse Botanical and Mycological Samples

Effortlessly extract DNA from a wide variety of plant materials, including leaf, stem, and root tissues, as well as from plant pathogens, fungi, and oomycetes. PhytoGEM™ is the ideal tool for studying plant genotypes, genetic variation, endophytic pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and verifying the authenticity of botanical extracts.

Overcomes Traditional Extraction Challenges

Conventional methods struggle with the polysaccharides, tannins, and polyphenols released from plant and fungal cells, requiring multi-step, time-intensive processes that often degrade DNA. PhytoGEM’s proprietary, temperature-controlled enzymatic lysis effectively breaks down tough cell walls in a closed-tube system. This gentle approach eliminates harsh chemical washes, preserves DNA integrity, and yields inhibitor-free DNA suitable for sensitive downstream applications.

Proactive Disease Control Through Rapid Detection of Critical Pathogens

Early detection is critical for crop management. PhytoGEM™, used with the automated PDQeX Nucleic Acid Extractor, transforms this bottleneck into an easy process. From a simple leaf punch on a storage card or homogenized tissue, the system performs precise, closed-tube lysis and purification, drastically reducing hands-on time and contamination risk.

Validated for Key Agricultural Threats

• The PhytoGEM™ method has been specifically validated for detecting a range of economically significant pathogens, including:
• Bacteria: Xylella fastidiosa, Pseudomonas syringae (Psa), Candidatus Liberibacter solanacearum
• Oomycetes: Phytophthora species
• Phytoplasma: In grapevines
• Viruses: Cassava Mosaic Virus
• Fungi: Providing high-quality DNA for genomics, strain characterization, and environmental studies 

Applications

• Plant samples
• Leaf, Stem, Root, Plant Pathogens, Fungi 

Kit Components

• PhytoGEM™
• Histosolv
• GREEN+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XPP0100, XPP0500, XPP1000)