ForensicGEM™ Sperm Enzymatic Kits

Precision and efficiency for forensic differential extraction.

Make the complex challenge of sperm cell lysis fast and simple. ForensicGEM™ Sperm is a specialized enzymatic kit designed for the rapid, single-tube extraction of high-quality DNA from sperm cells, semen samples, and vaginal swabs. Eliminating the need for harsh, inhibitory chemicals and lengthy purification helps achieve superior yields and STR-ready DNA in just 11 minutes.

Main Attributes

• Ultra-fast results, analysis-ready DNA in ~11 minutes.
• No purification or bead/column steps means no DNA loss, enabling work with very small cell numbers
• Inhibitor-free DNA: eliminates harsh chemicals like DTT that interfere with STR and qPCR, removing the need for post-lysis cleanup
• Single-tube, no-transfer processing safeguards results and minimizes the risk of contamination
• Frees technician time, reduces plasticware use, cuts costs, and is easily automated 

Reliable DNA Extraction from Sperm Cells

Sperm cells are notoriously difficult to lyse without compromising downstream analysis. This kit enables efficient differential extraction workflows and Y-screening, even from samples with very low sperm counts. The method produces high-quality double-stranded DNA for STR profiling, PCR, and qPCR.

Free of Downstream Inhibitory Compounds/Chemicals

Traditional methods rely on STR-inhibiting chemicals like DTT, SDS, and mercaptoethanol, which are toxic to downstream processes and require time-consuming purification steps that cause DNA loss. ForensicGEM™ Sperm uses a novel, temperature-controlled cocktail of thermophilic and mesophilic enzymes.

~11-Minute, Hands-Off process Completed in a Single Tube

This closed-tube system eliminates transfer steps, minimizing opportunities for contamination, cross-talk, and human error while protecting sample integrity. The protocol is compatible with existing thermal cyclers and easily automated for high-throughput processing, from low-volume casework to large-scale batches. This rapid processing means sex crime cases can be triaged very quickly to determine if a Y chromosome is present in the sample.

Applications

• Extraction from semen, including:
• Swab, Stain, Liquid 

Kit Components

• ForensicGEM™
• ORANGE+ Buffer
• Acrosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFS0100, XFS0500, XFS1000)