PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

Proudly made in the USA! Click here for more made in America products!

PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!

PR1MA™ β - Agarase

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.

Benefits

• Complete digestion of agarose, with no agarose fragments, left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
• Concentration: 1000 u / mL
• Operating temperature: 42°C to 50°C
• Recommended temperature: 50°C
• Storage temperature: -20°C 

Storage Buffer

• 50% glycerol
• 50 mM Tris-HCI
• 50 mM KCI
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!  

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Cell Separation Assembly Kit

Applications include immunology research, oncology research, neuroscience, stem cell research, and cell therapy.

RWD magnetic microbead cell separation products consist of Nano-Bead Cell Separation Platform and Nano-Beads (Column Based and Column free). Nano-sized magnetic beads do not need elution, and the separated cells can be directly used in downstream experiments such as flow cytometry, cell culture, single-cell sequencing, and more.

• High purity, high viability, and high recovery rate
• Obtained cells are compatible with downstream applications
• Mild, non-toxic, biodegradable

Kit Includes

• 1 box of LarSep Columns (25/pk)
• 1 LSC Separator (single channel)
• 1 Separation Stand

Work Flow

• Antibody incubation: Incubate the antibody with cell suspension at 4ºC for 10-15 mins.
• Microbead incubation: Incubate the magnetic microbeads with cell suspension at 4ºC for 10-15 mins.
• Magnetic separation: Put the separation column into the magnetic field and add the cell suspension into the column.
• Only cells labeled with magnetic beads will be captured in the column.
• Elution: Remove the column from the magnetic field and add a buffer to elute the positive cells.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Human Cell Separation Kits

CD3+ Cell Separation Kit - K1201-10

For positive selection or depletion of CD3+ T cells from peripheral blood, thymus, lymphoid, and tumor tissue.

CD4+ Cell Separation Kit - K1202-10

For positive selection or depletion of T helper cells (Th) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD8+ Cell Separation Kit - K1203-10

For positive selection or depletion of cytotoxic T cells (Tc) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD14+ Cell Separation Kit - K1204-10

For positive selection or depletion of monocytes from human peripheral blood, umbilical cord blood, spleen, lymph nodes, and other samples.

CD45+ Cell Separation Kit - K1205-10

For positive selection or depletion of white blood cells from human peripheral blood and lymphoid tissue samples.

CD19+ Cell Separation Kit - K1206-10

For positive selection or depletion of B cells from human peripheral blood, lymph nodes, bone marrow, and other single-cell suspensions.

Human NK Cell Isolation Kit - K1207-10

For negative selection or depletion of NK cells from single cell suspension of human peripheral blood. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and red blood cells are labeled by biotin antibodies coupled to magnetic beads. High purity NK cells are obtained by isolation of magnetic bead labeled cells.

*All kits are for research use only.

Specifications
 

Item #	Cell Type	Size	Capacity
K1201-10	T Cells	2 x 1 mL	For 1 x 10^9 Total Cells
K1202-10	T Helper Cells	2 x 1 mL
K1203-10	Cytotoxic T Cells	2 x 1 mL
K1204-10	Monocytes	2 x 1 mL
K1205-10	Leukocytes	2 x 1 mL
K1206-10	B Cells	2 x 1 mL
K1207-10	NK Cells	2 x 2 mL

 

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD High Activity Enzymatic Digestion Kits

Optimized enzymatic hydrolysis formulation for efficient and gentle digestion. Preserves the integrity of surface antigenic epitopes. Store in refrigerators at 4°C or -20°C.

• Targeted optimization of enzymatic hydrolysis formula can ensure high cell viability.
• Preserving cell surface epitopes leads to no effect on downstream experiments.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.   

RWD Mouse and Microglia Cell Separation Kits

Mouse CD3+ Cell Separation Kit - K1301-10

For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.

Mouse CD4+ Cell Separation Kit - K1302-10

For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.

Mouse CD8+ Cell Separation Kit - K1303-10

For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.

Mouse CD45+ Cell Separation Kit - K1304-10

For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.

Mouse CD19+ Cell Separation Kit - K1305-10

For positive selection or depletion of B cells from mouse spleen and other samples.

CD11b+ (Microglia) cell separation Kit - K1306-10

For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.

Specifications
 

Item #	Cell Type	Size	Capacity
K1301-10	T Lymphocytes	2 x 1 mL	For 1*10^9 Total Cells
K1302-10	T Helper Cells		For 1*10^9 Total Cells
K1303-10	Cytotoxic T Cells		For 1*10^9 Total Cells
K1304-10	Leukocytes		For 1*10^9 Total Cells
K1305-20	B Cells		For 2*10^9 Total Cells
K1306-10	Myeloid Cells		For 1*10^9 Total Cells

 

PrepGEM™ Universal DNA Extraction Kits

Single-tube, 15-minute DNA extraction solutions.

The PrepGEM™ Universal is a comprehensive DNA extraction kit designed for maximum yield, integrity, and ease of use. Engineered for researchers and technicians who demand efficiency and reliability, this adaptable reagent kit delivers high-quality DNA from both common and challenging sample types, all in a single tube.

Main Attributes

• Single tube simplifies processes, no transfers, no washes, no DNA loss
• Get rapid results from sample to DNA in ~15 minutes
• Broad compatibility optimized for blood, saliva, tissue, and cell culture
• Ideal for CRISPR genotyping and a wide range of downstream analyses
• Reduced plastic waste and hazardous chemicals for sustainable science

Simple and Effortless Extraction from Any Source

Consistently extract high-yield DNA from blood, saliva, tissues (including ear punches, mouse tails, and insects), and cell cultures. Our closed-tube, inhibitor-free method eliminates harsh chemical washes and multiple steps, ensuring no DNA is lost. This makes prepGEM™ Universal exceptionally powerful for low-cell-number extractions, down to a single cell.

Ideal for Advanced Genotyping and Sequencing

PrepGEM™ Universal is the perfect tool for performing confirmatory genotyping of transfected cells after CRISPR/Cas9 gene editing or preparing DNA for downstream applications. The kit produces ready-to-use DNA for STR analysis, PCR, qPCR, whole genome amplification, and amplicon sequencing.

Every Lab Deserves Rapid Results

Activate our proprietary thermophilic proteinase with a simple temperature change. The entire hands-off process is easily automated in a single tube using a standard thermocycler and takes approximately 15 minutes. Free up valuable technician time, reduce lab costs, and significantly cut down on plastic and hazardous waste.

Applications

• Blood Samples
• Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
• Saliva Samples
• Liquid, Buccal swab, Stains, Storage Card (FTA)
• Tissue Samples
• Tissue cultures, including single cell, Animal (scarpings, fat, muscle), Mouse and rat tail tips, Ear tags, Fish fins, Insects 

Kit Components

• PrepGEM™
• Histosolv
• BLUE Buffer
• RED+ Buffer
• ORANGE+ Buffer
• PDQeX Kits Include PDQeX Kit Tubes (XPU0100, XPU0500, XPU1000)

PrepGEM™ Bacterial Enzymatic Kits

Speed and gentle precision for unparalleled insight into the microbial world.

The PrepGEM™ Bacteria enzymatic kit is designed for efficient, high-quality DNA extraction from a vast array of microbial sources. Engineered for microbiologists and metagenomics researchers, this method combines gentle lysis with a closed-tube workflow to preserve DNA integrity and ensure accurate community representation, all in a fraction of the time of traditional methods.

Main Attributes

• Enzymatic combination ensures gentle and efficient lysis while preserving DNA integrity
• Rapid results in a quarter of the time of conventional methods
• True Community Representation: Closed-tube, no-transfer process protects low-abundance species in metagenomic samples
• Broad compatibility from pure cultures to complex environmental samples
• Easily automated for high throughput in a secure, closed system for safety and scalability 

Comprehensive Microbial and Metagenomic Profiling

Efficiently extract DNA from Gram-positive and Gram-negative bacteria, protozoa, archaea, colonies, and liquid cultures. The kit is equally effective for complex samples like biofilms, mucosal swabs, and environmental matrices, including soil, stool, and water. It is the ideal choice for metagenomic studies.

Integrity for Demanding Applications

The gentle, temperature-driven enzymatic lysis uses a unique combination of thermophilic proteinase and mesophilic lysozyme to minimize DNA fragmentation. With no mechanical disruption or transfer steps, you obtain exceptionally intact, high-molecular-weight DNA that accurately represents low-abundance species. The resulting DNA is ready for PCR, qPCR, targeted amplicon sequencing such as 16S rDNA analysis, and other downstream applications.

Accelerated Research Process, 1/4 the Time of Other Methods

The fully enclosed, single-tube system eliminates cross-contamination, protects users from pathogens, and requires minimal hands-on time. The automation-ready workflow reduces pipetting, preserves sample integrity, and drastically cuts down on plastic waste.
 

Applications

• Extraction from a variety of microbial samples including:
• Gram-positive and -negative bacteria, protozoa, colonies and liquid cultures, biofilm and mucosal, archaea, swabs, and metagenomic DNA: soil, stool, water 

Kit Components

• PrepGEM™
• GREEN+ Buffer
• Lysozyme
• WASH+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XBA0100, XBA0500, XBA1000)

ForensicGEM™ Universal Enzymatic Kits

Precise, efficient, and secure solution for modern forensic DNA analysis.

ForensicGEM™ Universal is a DNA extraction kit designed for fast, reliable results in forensic science. Where speed, sample integrity, and minimal contamination are paramount, our innovative enzymatic process delivers analysis-ready DNA from challenging forensic samples in just 15 minutes.

Main Attributes

• Obtain STR, PCR, and qPCR-ready DNA in approximately 15 minutes
• Eliminating purification steps enables efficient extraction from very small sample volumes
• Single-tube, closed processing protects sample integrity and minimizes contamination risks
• Reduced hands-on time, lab costs, consumable use, and plastic waste
• Compatible with standard lab equipment for easy automation and adoption 

Optimized for a Comprehensive Range of Forensic Evidence Types

Consistently extract high-yield DNA from saliva (liquid, swabs, stains, FTA cards), blood (liquid, swabs, stains, FTA cards), and tissue (solid samples and hair). The process is uniquely effective for both small-volume and trace samples.

Superior DNA for Demanding Forensic Analysis

The method produces DNA that is PCR-ready and suitable for STR profiling. Unlike traditional methods, our temperature-controlled process uses a novel cocktail of enzymes instead of harsh detergents and reducing agents like SDS or DTT. This eliminates common PCR inhibitors, ensuring superior results without purification steps.

Fully Hands-Off, Single-Tube Workflow

From sample to analysis, the process is contained, drastically reducing opportunities for contamination and protecting sample integrity. This approach is perfect for high-volume casework where quick turnaround is critical. The method is easily automated using existing thermal cyclers, freeing up valuable technician time and accelerating response times.

For extracting DNA from sperm, use ForensicGEM™ Sperm.
 

Applications

• Extraction from a variety of forensic blood samples
• Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
• Tissue samples
• Animal (scrapings, fat, and muscle), Tape lifts, Hair
• Saliva samples
• Liquid, Swab, Stains, Storage Card (FTA) 

ForensicGEM™ is a preparative method for DNA extraction. The method lyses cells and removes nucleoproteins from the DNA. Extracted DNA can be used for many types of genotyping, including SNP and STR analysis as well as quantitative, multiplex, and endpoint PCR.

There is no concentration step in the procedure, so the concentration of the extract is dependent on:

• The quality of the sample
• In the case of swabs, the type of swab and the volume of water used to wash the swab
• The extraction volume (which in some cases can be scaled)

DNA extracted using ForensicGEM™ is largely single stranded because of the 95ºC heat step. For accurate yield assessment, a qPCR is recommended. If standard fluorescent chelating dyes are to be used for normalising samples, then we recommend taking a sample of the extract before the 95ºC step. Alternatively, you can generate a standard curve using a previously made extract that has been quantified.

Kit Components

• ForensicGEM™
• BLUE Buffer
• RED+ Buffer
• ORANGE+ Buffer
• Histosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFU0100, XFU0500, XFU1000)

ForensicGEM™ Sperm Enzymatic Kits

Precision and efficiency for forensic differential extraction.

Make the complex challenge of sperm cell lysis fast and simple. ForensicGEM™ Sperm is a specialized enzymatic kit designed for the rapid, single-tube extraction of high-quality DNA from sperm cells, semen samples, and vaginal swabs. Eliminating the need for harsh, inhibitory chemicals and lengthy purification helps achieve superior yields and STR-ready DNA in just 11 minutes.

Main Attributes

• Ultra-fast results, analysis-ready DNA in ~11 minutes.
• No purification or bead/column steps means no DNA loss, enabling work with very small cell numbers
• Inhibitor-free DNA: eliminates harsh chemicals like DTT that interfere with STR and qPCR, removing the need for post-lysis cleanup
• Single-tube, no-transfer processing safeguards results and minimizes the risk of contamination
• Frees technician time, reduces plasticware use, cuts costs, and is easily automated 

Reliable DNA Extraction from Sperm Cells

Sperm cells are notoriously difficult to lyse without compromising downstream analysis. This kit enables efficient differential extraction workflows and Y-screening, even from samples with very low sperm counts. The method produces high-quality double-stranded DNA for STR profiling, PCR, and qPCR.

Free of Downstream Inhibitory Compounds/Chemicals

Traditional methods rely on STR-inhibiting chemicals like DTT, SDS, and mercaptoethanol, which are toxic to downstream processes and require time-consuming purification steps that cause DNA loss. ForensicGEM™ Sperm uses a novel, temperature-controlled cocktail of thermophilic and mesophilic enzymes.

~11-Minute, Hands-Off process Completed in a Single Tube

This closed-tube system eliminates transfer steps, minimizing opportunities for contamination, cross-talk, and human error while protecting sample integrity. The protocol is compatible with existing thermal cyclers and easily automated for high-throughput processing, from low-volume casework to large-scale batches. This rapid processing means sex crime cases can be triaged very quickly to determine if a Y chromosome is present in the sample.

Applications

• Extraction from semen, including:
• Swab, Stain, Liquid 

Kit Components

• ForensicGEM™
• ORANGE+ Buffer
• Acrosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFS0100, XFS0500, XFS1000)

PhytoGEM™ Enzymatic Kits

Robust, automated solution for modern plant science and mycology.

Eliminate the challenges of plant and fungal DNA extraction with phytoGEM™, the specialized kit designed for the automated, high-quality isolation of DNA from even the toughest samples. From resilient leaf tissue to complex fungal pathogens, our enzymatic lysis method overcomes inhibitory compounds and tough cell walls, delivering PCR and qPCR-ready DNA without laborious grinding, columns, or hazardous solvents.

Main Attributes

• Enzymatic lysis efficiently breaks down tough cells, handling plant and fungal cell walls without mechanical grinding
• Produces clean DNA free from polysaccharides and polyphenols that inhibit PCR
• Enables simple, closed-tube processing on the PDQeX system for high consistency and safety
• Accelerates pathogen detection and genetic analysis from hours to minutes for rapid results
• Trusted for key plant diseases and fungal genomics 

Designed for Diverse Botanical and Mycological Samples

Effortlessly extract DNA from a wide variety of plant materials, including leaf, stem, and root tissues, as well as from plant pathogens, fungi, and oomycetes. PhytoGEM™ is the ideal tool for studying plant genotypes, genetic variation, endophytic pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and verifying the authenticity of botanical extracts.

Overcomes Traditional Extraction Challenges

Conventional methods struggle with the polysaccharides, tannins, and polyphenols released from plant and fungal cells, requiring multi-step, time-intensive processes that often degrade DNA. PhytoGEM’s proprietary, temperature-controlled enzymatic lysis effectively breaks down tough cell walls in a closed-tube system. This gentle approach eliminates harsh chemical washes, preserves DNA integrity, and yields inhibitor-free DNA suitable for sensitive downstream applications.

Proactive Disease Control Through Rapid Detection of Critical Pathogens

Early detection is critical for crop management. PhytoGEM™, used with the automated PDQeX Nucleic Acid Extractor, transforms this bottleneck into an easy process. From a simple leaf punch on a storage card or homogenized tissue, the system performs precise, closed-tube lysis and purification, drastically reducing hands-on time and contamination risk.

Validated for Key Agricultural Threats

• The PhytoGEM™ method has been specifically validated for detecting a range of economically significant pathogens, including:
• Bacteria: Xylella fastidiosa, Pseudomonas syringae (Psa), Candidatus Liberibacter solanacearum
• Oomycetes: Phytophthora species
• Phytoplasma: In grapevines
• Viruses: Cassava Mosaic Virus
• Fungi: Providing high-quality DNA for genomics, strain characterization, and environmental studies 

Applications

• Plant samples
• Leaf, Stem, Root, Plant Pathogens, Fungi 

Kit Components

• PhytoGEM™
• Histosolv
• GREEN+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XPP0100, XPP0500, XPP1000)