SmartDye™ Terminator v3.1 Cycle Sequencing Kits

SmartDye™ Terminator v3.1 Cycle Sequencing Kits are engineered to deliver exceptional Sanger sequencing performance with longer, high-quality reads, superior accuracy, and minimal background noise. Built on the trusted Sanger sequencing method, SmartDye™ chemistry ensures reliable sequencing even on difficult templates, including GT-rich regions.

Designed as a direct, economical drop-in replacement for the Applied Biosystems, BigDye™ Terminator v3.1 Cycle Sequencing Kit, SmartDye™ requires no protocol changes, no new mobility shift files, and no recalibration, making the transition seamless for any laboratory.

SmartDye™ Terminator v3.1’s proprietary fluorescently labeled dideoxynucleotide mix, high-fidelity DNA polymerase, and 5X optimized buffer combine to deliver long read lengths and high signal-to-noise ratios. This ensures clean, accurate data interpretation even when sequencing challenging templates.

Main Attributes

• Robust Chemistry
• Based on proven Sanger sequencing technology
• High-quality, long read lengths with low error rates
• Optimized dye chemistry for clear signal separation
• Ideal for templates with high GT repeat content

Flexible Applications

SmartDye™ supports multiple sequencing workflows, including:

• De novo sequencing
• Resequencing
• Finishing applications

Compatible with a wide range of templates:

• PCR products
• Plasmids
• BACs
• Fosmids

Direct Replacement for BigDye™

• Fully compatible with existing BigDye™ v3.1 protocols
• Simply substitute SmartDye™ for BigDye™ no retraining or workflow changes
• Faster reaction times possible (as little as 3 minutes) without sacrificing quality 

Cost-Effective Solution

SmartDye™ offers excellent value with ready-to-use premix and 5X buffer. Kits are available in 24, 100, 1,000, 5,000, and 25,000 reaction sizes, supporting labs of all throughput levels. Research also shows SmartDye™ performs well at reduced volumes, as little as 0.15 uL in a 5 uL reaction, further lowering per-reaction cost.

Disclaimer
BigDye™ is a trademark of Applied Biosystems, LLC. SmartDye™ Terminator Sequencing Kits are thirdparty products designed for use in workflows compatible with BigDye™ chemistry. Applied Biosystems, LLC does not manufacture, sponsor, or endorse SmartDye™ products, and is not affiliated with these offerings in any way.
 

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Accuris qMAX™ One-Step RT-qPCR kits

• RNA to cDNA to qPCR, in one tube
• High purity enzyme formulation for enhanced stability and performance
• Accuris Hot-Start Taq allows for preparation at room-temperature
• Blue dye facilitates pipetting and visualization in plates
• Available for green fluorescence or probe detection
• Multiplex formulation available for multiple target amplification
• Bulk pricing available for high throughput labs and kit manufacturers. Contact us for details

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX™ Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX™ Probe One Step kits are optimized for use with popular TaqMan™, Scorpions, and molecular beacon probes.

qMAX™ Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

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PR1MA™ Tn5 2.0 Transposase

PR1MA™ Tn5 2.0 Transposase (Tnp) is a hyperactive retroviral integrase engineered for improved activity, speed, and robustness that is used to construct random next-generation sequencing libraries and to study chromatin structure using targeted ATAC-seq. PR1MA™ Tn5 2.0 can be used to randomly fragment any target and insert unique oligonucleotide adapters in a single reaction which reduces the time and sample requirements relative to traditional next-gen sequencing library construction. 

• Optimal temperature: 55°C
• Inactivation: 40X Stop solution included (2% SDS)
• Storage temperature: -20°C
• 10X Tn5 reaction buffer included

Buffer composition

• 100 mM Tris-HCl
• 100 mM MgCl2
• pH = 7.5

*These products are intended for research use only, not for diagnostic use. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

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PR1MA™ Taq DNA Polymerase

Saves time and cost by enabling direct PCR amplification of unpurified templates. PR1MA™ Taq DNA polymerase is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays. It is supplied with 4 M betaine to improve amplification of GC-rich DNA and 30% sucrose to improve amplification from inhibitor-rich substrates such as blood.

• Lacks exonuclease activity
• Thermotolerant up to 98°C
• Resistant to inhibitors, e.g., whole blood
• Ideal for GC-rich templates
• Storage temperature: -20°C
• 10X PR1MA™ buffer, 4 M Betaine, and 30% Sucrose included

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.5% Tween-20
• 0.5% NP-40 substitute
• pH = 7.5 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

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PR1MA™ CRISPR/Cas9 Nucleases

The RNA-guided endonuclease Cas9, associated with Type II CRISPR/Cas systems, site-specifically digests DNA using a single guide RNA (sgRNA) which it binds to direct it to the complementary sequence. MIDSCI™ offers two traditional versions of Streptococcus pyogenes Cas9 nuclease: CRISPR/Cas9, ideal for in vitro DNA digestion, and CRISPR/Cas9 NLS, for in vivo nuclear localization.

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ Bst DNA Polymerase

PR1MA™ Bst polymerase (patent pending) is a recombinant, truncated, thermostable Bacillus stearothermophilus DNA polymerase with high reverse transcriptase and strand-displacement activities, ideal for isothermal amplification of RNA and DNA targets. PR1MA™ Bst polymerase has increased sensitivity and speed relative to other Bst polymerases and can incorporate dUTP. 

Use PR1MA™ Bst polymerase to develop LAMP assays with high sensitivity and specificity.

• Lacks 5’ to 3’ exonuclease activity
• Only Bst polymerase in the market with robust RT and DNA polymerase activity
• Thermostable, working temperature range 64 - 72°C
• Tolerant to inhibitors
• Storage temperature: - 20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCls
• 1 mM DTT
• 0.1  mM EDTA
• 0.05% Tween – 20
• 0.05% NP - 40 substitute
• pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.  

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PR1MA™ Reverse Transcriptase

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase that can be used for complementary DNA (cDNA) synthesis from an RNA template and is ideal for use in molecular amplification assays. PR1MA™ RT is a robust enzyme that works in a broad range of temperatures (40 - 72°C) and has RNase H activity.

• Optimal temperature: 55°C
• Heat inactivation: 75°C for 20 minutes
• Glycerol-free buffer available
• Storage temperature: -20°C (standard buffer)
• 10X Isothermal buffer included

Standard Buffer Composition

• 50% glycerol
• 10 mM Tris-HCl
• 100 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

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PR1MA™ Cod Uracil-DNA Glycosylase

PR1MA™ Cod Uracil-DNA Glycosylase (cUNG) is a recombinant, thermolabile enzyme that removes uracil from DNA. It is ideal for preventing carry over contamination during RNA or DNA amplification reactions that substitute dUTP for dTTP. cUNG is the only commercially available UNG that is completely and irreversibly inactivated by moderate heat treatment, unlike bacterial versions of the enzyme. Cod UNG treatment in combination with targeted pre-amplification using dUTP provides a simple and efficient solution to eliminate carry-over contamination and the generation of false positives and inaccurate quantification.

• Optimal temperature: 37°C
• Heat inactivation: 55°C for 5 minutes
• Enables contamination control in PCR and other amplification methods
• Does not degrade product after inactivation, enabling downstream use of the amplicon
• Storage temperature: -20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.1% Tween-20
• pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

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PR1MA™ Reverse Transcriptase, RNase H -

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.

• Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
• Lacks 3’ to 5’ exonuclease activity
• Optimal temperature at 42°C
• Temperature range: 40 to 50°C
• Heat inactivation: 70°C for 20 minutes
• Storage temperature: -20°C
• 10X reaction buffer included 

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

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PR1MA™ RNA Controls

Looking for safe assay controls for highly infectious viruses or foreign animal diseases?

MIDSCI™ offers a selection of PR1MA™ RNAs – nuclease-resistant, single-stranded RNAs, suitable as process controls for RNA extraction from various sample matrices. These specially engineered, non-infectious, MS2 phage-like particles protect their contents from degradation by nucleases and can package sequences of up to 1.5 kb from viruses such as SARS-CoV-2, foot-and-mouth disease virus, and human immunodeficiency virus.
 

Amount: Between 1E6 or 1E10 copies (cp)
Concentration: Between 1E7 or 1E10 cp / mL, respectively
Volume: 0.1 and 1 mL respectively
Lysis: 65° C for 5 minutes in the RT step or by standard RNA extraction
Storage temperature: 4°C

Buffer composition

• 10 mM Tris HCl
• 100 mM NaCl
• 1 mM MgCl2
• 0.1% gelatin
• pH = 7.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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