SmartDye™ Terminator v3.1 Cycle Sequencing Kits

SmartDye™ Terminator v3.1 Cycle Sequencing Kits are engineered to deliver exceptional Sanger sequencing performance with longer, high-quality reads, superior accuracy, and minimal background noise. Built on the trusted Sanger sequencing method, SmartDye™ chemistry ensures reliable sequencing even on difficult templates, including GT-rich regions.

Designed as a direct, economical drop-in replacement for the Applied Biosystems, BigDye™ Terminator v3.1 Cycle Sequencing Kit, SmartDye™ requires no protocol changes, no new mobility shift files, and no recalibration, making the transition seamless for any laboratory.

SmartDye™ Terminator v3.1’s proprietary fluorescently labeled dideoxynucleotide mix, high-fidelity DNA polymerase, and 5X optimized buffer combine to deliver long read lengths and high signal-to-noise ratios. This ensures clean, accurate data interpretation even when sequencing challenging templates.

Main Attributes

• Robust Chemistry
• Based on proven Sanger sequencing technology
• High-quality, long read lengths with low error rates
• Optimized dye chemistry for clear signal separation
• Ideal for templates with high GT repeat content

Flexible Applications

SmartDye™ supports multiple sequencing workflows, including:

• De novo sequencing
• Resequencing
• Finishing applications

Compatible with a wide range of templates:

• PCR products
• Plasmids
• BACs
• Fosmids

Direct Replacement for BigDye™

• Fully compatible with existing BigDye™ v3.1 protocols
• Simply substitute SmartDye™ for BigDye™ no retraining or workflow changes
• Faster reaction times possible (as little as 3 minutes) without sacrificing quality 

Cost-Effective Solution

SmartDye™ offers excellent value with ready-to-use premix and 5X buffer. Kits are available in 24, 100, 1,000, 5,000, and 25,000 reaction sizes, supporting labs of all throughput levels. Research also shows SmartDye™ performs well at reduced volumes, as little as 0.15 uL in a 5 uL reaction, further lowering per-reaction cost.

Disclaimer
BigDye™ is a trademark of Applied Biosystems, LLC. SmartDye™ Terminator Sequencing Kits are thirdparty products designed for use in workflows compatible with BigDye™ chemistry. Applied Biosystems, LLC does not manufacture, sponsor, or endorse SmartDye™ products, and is not affiliated with these offerings in any way.
 

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