2026 On Target Promo!

 

For a limited time, buy one AccuFi™ High Fidelity Hot Start Master Mix (PR1002-HFHS-100)
and get ONE FREE (PR1002-HFHS-100-VF)

  

For free product, add item PR1002-HFHS-100 at $155.00

PLUS item PR1002-HFHS-100-VF to your cart.

 

Use promo code: OT202

 

Click here for more On Target savings

or see flyer for more details.

 

Promotion valid until 10/31/26 or while supplies last, for eligible U.S. customers.

Prices are in U.S. dollars and exclude freight, taxes, installation, and other applicable charges.

Offers may not be combined with other offers, discounts, or institutional pricing.

Customer must pay the list price on the qualifying item to receive the free item (List Price is reflected in flyer).

Buy-one-get-one offers apply to identical eligible catalog numbers unless otherwise stated.

All terms and conditions apply.

AccuFi™ High Fidelity Hot Start Master Mix

Highly accurate amplification.

Proofreading enzymes are modified for better sensitivity and higher activity to ensure more reproducible performance with a wide range of targets. 

• Up to 100X higher fidelity than Taq polymerase
• Crude DNA sample will not inhibit amplification
• Ideal for cloning, mutagenesis, sequencing, and microarrays
• Options for blunt-end or A-overhang products
• Optimized buffers include PCR enhancers 

Accuris™ AccuFi™ HFHS PCR Mix is an optimized 2X master mix that includes AccuFi™ High Fidelity polymerase, a proprietary reaction buffer system, MgCl2, and ultra-pure dNTPs. The engineered polymerase exhibits rapid extension kinetics (~10 s/kb), enabling efficient fast cycling protocols while maintaining high processivity. The formulation supports robust long-range amplification, with reliable performance on templates exceeding 15 kb.

The enzyme possesses both 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading activity, resulting in an ultra-low error rate (below the detection limit) compared to Taq DNA polymerase. The high fidelity of the enzyme supports applications where sequence integrity is critical, such as molecular cloning, next-generation sequencing (NGS) library construction, and site-directed mutagenesis.

The optimized buffer system enhances amplification efficiency across challenging templates, including GC-rich and partially impure DNA samples, while maintaining high yield and specificity with minimal reaction optimization.

Main Attributes

• Ultra-high fidelity: Exhibits highly accurate DNA synthesis supported by coupled 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading
• Hot Start DNA polymerase: Minimizes nonspecific amplification, reduces background, and enhances reproducibility in PCR
• Strong amplification performance: Supports efficient amplification across challenging templates, including GC-rich sequences, low-quality, and long-range targets, while maintaining high yield
• Fast extension rate: Delivers rapid DNA synthesis at approximately 1 kb/10 seconds, supporting accelerated PCR cycling 

Storage
 

Upon receipt, immediately store at -20ºC. Avoid excessive freeze/thaw cycles.
 

Proudly made in the USA! Click here for more made in America products!