AccuFi™ High Fidelity Hot Start Master Mix

Highly accurate amplification.

Proofreading enzymes are modified for better sensitivity and higher activity to ensure more reproducible performance with a wide range of targets. 

• Up to 100X higher fidelity than Taq polymerase
• Crude DNA sample will not inhibit amplification
• Ideal for cloning, mutagenesis, sequencing, and microarrays
• Options for blunt-end or A-overhang products
• Optimized buffers include PCR enhancers 

Accuris™ AccuFi™ HFHS PCR Mix is an optimized 2X master mix that includes AccuFi™ High Fidelity polymerase, a proprietary reaction buffer system, MgCl2, and ultra-pure dNTPs. The engineered polymerase exhibits rapid extension kinetics (~10 s/kb), enabling efficient fast cycling protocols while maintaining high processivity. The formulation supports robust long-range amplification, with reliable performance on templates exceeding 15 kb.

The enzyme possesses both 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading activity, resulting in an ultra-low error rate (below the detection limit) compared to Taq DNA polymerase. The high fidelity of the enzyme supports applications where sequence integrity is critical, such as molecular cloning, next-generation sequencing (NGS) library construction, and site-directed mutagenesis.

The optimized buffer system enhances amplification efficiency across challenging templates, including GC-rich and partially impure DNA samples, while maintaining high yield and specificity with minimal reaction optimization.

Main Attributes

• Ultra-high fidelity: Exhibits highly accurate DNA synthesis supported by coupled 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading
• Hot Start DNA polymerase: Minimizes nonspecific amplification, reduces background, and enhances reproducibility in PCR
• Strong amplification performance: Supports efficient amplification across challenging templates, including GC-rich sequences, low-quality, and long-range targets, while maintaining high yield
• Fast extension rate: Delivers rapid DNA synthesis at approximately 1 kb/10 seconds, supporting accelerated PCR cycling 

Storage
 

Upon receipt, immediately store at -20ºC. Avoid excessive freeze/thaw cycles.
 

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