PR1MA™ Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
• 1.5~2.5 μL per well for general Western blotting transfer

The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)

PR1MA™ Tricolor Protein Markers

PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μL per well for general Western blotting transfer.

The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Specifications

Item#	Color	Bands	Range
PR5010-BL	Blue	10	9-180 kDA
PR5011-BL	Blue	11	9-180 kDA
PR5010-TC	Tri-Color	10	9-180 kDA
PR5012-TC	Tri-Color	12	9-245 kDA
PR5013-TC	Tri-Color	13	9-245 kDA

 

PR1MA™ IPTG [Isopropyl-β-D-thiogalactopyranoside], Dioxane Free

Common Name: Isopropyl-β-D-thiogalactopyranoside
CAS Number: 367-93-1
Molecular Weight: 238.3
Chemical Formula: C₉H₁₈O₅S
Solubility: Water, ethanol
Storage Temp: 2-8°C

IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.

Dioxane-Free
Water Content: <0.49%

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (HPLC): 98.0 - 101.0 %
• Purity (HPLC): ≥ 99.0 %
• 1,4-Dioxane: Not Detected
• Absorbance @300nm (5% w/v sol in H₂O): ≤ 0.13
• Absorbance @400nm (5% w/v solution in H₂O): ≤ 0.06
• Identification: IR Concordant with Standard
• Melting Range: 110 - 114 °C
• Moisture Content (KF): ≤ 1.0 %
• pH (5% in water): 5.0 - 7.0
• Solubility: Soluble in Water and Methanol
• Specific Optical Rotation: -34.5 - -28.5 ^o
• BSE/TSE Free: BSE/TSE Free
• Non Animal Origin: Non Animal Origin
• Country of Origin: Report

PR1MA™ Phosphate Buffered Saline, 10X Solution

• Appearance: Clear and Colorless Solution
• Composition: Sodium Chloride: 1.37M, Potassium Chloride: 0.027M, Phosphate Buffer: 0.12M
• Method: Prepared in 18.2 megaohams-cm ± 1 water and filtered through 1 micron filter
• pH (1X Solution): 7.2 - 7.6
• pH (10X Solution): 6.8 - 7.2

PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder, 50:1 Ratio

Storage Temp: Room Temperature

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• Solubility: Clear Solution
• Conductivity: ≤ 10 µmhos
• Electrophoresis: Pass

PR1MA™ Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C₃H₈O₃
Solubility: Water
Storage Temp: Room Temperature

Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Liquid
• Chloride (Cl): ≤ 0.001 %
• Chlorinated Compounds: ≤ 0.003 %
• Color, APHA: ≤ 10.0
• Fatty Acids & Esters (USP): ≤ 1.0 (ml 0.5 N NaOH) %
• Glycerine: ≥ 99.7 %
• Heavy Metals: ≤ 5.0 ppm
• Identification C (GC): Pass
• Limit of Diethylene Glycol & RC: Pass
• Purity (USP): 99.0 - 101.0 %
• Residue on Ignition: ≤ 0.01 %
• Specific Gravity: ≥ 1.259
• Water: ≤ 5.0 %
• Date of Manufacture: Report

PR1MA™ Agar, Phyto, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Specially selected and tested agar for use in plant tissue culture. High gel strength.

Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).

Research or further manufacturing use only, not for food or drug use.

• Appearance: Pale Yellow Powder
• Gelling Point: 34 - 36 °C
• Gel Strength : 950 - 1050 g/cm²
• Identification: Conforms
• Loss on Drying: ≤ 18 %
• Melting Point: 85 - 95 ^oC
• Nephelos: ≤ 40 NTU
• pH: 6.0 - 7.5
• Total Ash: ≤ 3.5 %
• Gelatin: Absent
• Starch: Absent
• Total Plate Count: ≤ 1000 cfu/g

PR1MA™ Casamino Acids [Casein acid hydrolysate]

Common Name: Casein acid hydrolysate
CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Casein Acid Hydrolysate is a hydrochloric acid digest of casein that contains small amounts of cystine and Tryptophan. All vitamins have been eliminated by the acidification treatment. Provides a source of amino acids as nutrients for growing various organisms tolerant of high salt conditions.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous, Free Flowing Powder
• Color: Light Tan Yellow
• Amino Nitrogen: ≥ 4.0 %
• Moisture : ≤ 6.0 %
• pH: 6.0 - 7.5
• Protein: Report %
• Solution (2%): Clear Light Yellow
• Total Nitrogen: > 7.5 %
• Coliforms: < 10 cfu/g
• Standard Plate Count : < 5000 cfu/g
• Thermophile Count: Report cfu/g
• Source: Bovine

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PR1MA™  BHI Broth [Brain Heart Infusion Broth], Powder

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PR1MA™ Gelrite™

CAS Number: 71010-52-1
Solubility: Water
Storage Temp: Room Temperature

Naturally derived gelling polymer that can be used in place of agar. Gelrite forms clear, rigid gels at approximately half the use level of agar in presence of soluble salts like Mg2+ and Ca2+.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Solid
• Gel Strength: 400 - 700 g/cm²
• Loss on Drying: ≤ 15.0 %
• Particle Size 42 Mesh (355 µm): ≥ 95 %
• Transmittance: ≥ 80.0 %

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PR1MA™ Taq

• Ideal for routine PCR applications as well as genotyping, colony PCR, and fast PCR
• Improved template affinity and solubility for higher enzyme activity and greater yields
• Proprietary buffer optimized for a variety of assay conditions
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer 

PR1MA™ Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA™ Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready-to-use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

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2026 On Target Promo!

 

For a limited time, buy 2 PR1MA™ SmartGlow™ DNA Safe Stains (BE4500-PS OR BE4500-LD)
and get 1 FREE 100g Benchmark™ Agarose LE (BE-GCA100-VF)!

 

MIX AND MATCH!

  

For free product, add the following items to your cart:
 

(2) BE4500-PS or BE4500-LD at $129.35
PLUS (1) BE-GCA100-VF item

 

Use promo code: OT202

 

Click here for more On Target savings

or see flyer for more details.

 

Promotion valid until 10/31/26 or while supplies last, for eligible U.S. customers.

Prices are in U.S. dollars and exclude freight, taxes, installation, and other applicable charges.

Offers may not be combined with other offers, discounts, or institutional pricing.

Customer must pay the list price on the qualifying item to receive the free item (List Price is reflected in flyer).

Buy-one-get-one offers apply to identical eligible catalog numbers unless otherwise stated.

All terms and conditions apply.

PR1MA™ SmartGlow™ DNA Safe Stain

• Replaces hazardous Ethidium Bromide (EtBr)
• Better sensitivity than EtBr
• Detect as little as 0.1ng of DNA
• Two types available
• PS Pre Stain
• LD Loading Dye
• Excitation by UV light or blue light
• Compatible with Accuris SmartBlue™ Transilluminator
• Ships at ambient temperature (stored at 4°C)

PR1MA™ SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA™ SmartStain™ PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.

Protocol:

• Prepare 100 mL of agarose or polyacrylamide solution.
• Add 5 uL of PR1MA™ SmartStain™ to the gel solution before pouring gels.
• For enhanced results, add PR1MA™ SmartStain™ PS to the running buffer at a ratio of 5 uL per 100 mL. Adding PR1MA™ SmartStain™ PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
• After electrophoresis is complete, view the gel using a UV or blue light illuminator

Harvest Fetal Bovine Serum (FBS)

Fetal Bovine Serum is collected entirely from USDA-licensed abattoirs and processed within the United States.

• High-quality FBS at an affordable price
• Store at -20ºC 
• Low Endotoxin lots (< 1.0 EU / mL) may be available. 
  
• From USDA-licensed facilities inside and outside the U.S.
• Tested for Growth Promotion*
• Large lot sizes
• Virus Screened-Mycoplasma Tested
• Triple 0.1 uM Filtered
• Endotoxin <  2.0 EU / mL
• Low Hemoglobin lots (< 15 mg / dL) may be available.

Specifications

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