PR1MA™ SOB Broth, Powder

Solubility: Water
Storage Temp: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.

Add 28 grams of Broth in 1 liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes.

Ingredients:

• Casein Enzymic Hydrolysate: 20 g/L
• Magnesium Sulfate: 2.4 g/L
• Yeast Extract: 5 g/L
• Potassium Chloride: 0.186 g/L
• Sodium Chloride: 0.5 g/L
• Final pH(25°:C): 7.0+0.2

• DCM Appearance: Homogeneous Free Flowing,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.8 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

Common Name: Polyoxyethylenesorbitan Monolaurate
CAS Number: 9005-64-5
Solubility: Water
Storage Temp: Room Temperature

Fatty acid composition:

• Lauric acid: Approximately 50%
• Balance: Myristic, Palmitic and Stearic acids.

Shipping Weight: 1.2 lbs
Shipping Dimensions: 2.00 x 6.00 x 2.00

Specifications

Appearance: Yellow to amber viscous liquid
1,4-Dioxane: ≤ 10 ppm
Acid Value: ≤ 2.20 mg KOH/g
Color, Gardner: ≤ 5.0
Hydroxyl Value: 96 - 108 mgKOH/g
Saponification Value: 40.0 - 50.0 mg KOH/g
Water: ≤ 3.00 %

Research or further manufacturing use only, not for food or drug use.

Proudly made in the USA! Click here for more made in America products!

PR1MA™ Miller's LB Agar Capsules

• Appearance: Clear Colorless Solution
• Absorbance @ 280nm (1mg/mL in H₂O): ≤ 0.015 nm
• Absorbance @ 570nm (100mg/mL in H₂O): ≤ 0.10 nm
• Potency (Dried basis): ≥ 700 µg/mg
• Sterility: Filtered through 0.22 µm filter Into sterile PETG bottles

PR1MA™  TAE 10X Solution [TRIS Acetate EDTA 10X Solution]

Common Name: TRIS Acetate EDTA 10X Solution
CAS Number: 77-86-1, 64-19-7, 6381-92-6, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

10X solution contains:

• TRIS-Base: 0.4 M
• Acetic Acid: 0.2 M
• EDTA: 10 mM
• pH (10X Solution (@25°C): 8.2-8.4

• Appearance: Clear, Colorless Solution
• pH: 7.8 - 8.2

Electrophoresis System Bundle

Package Includes:

• Complete PR1MA™ myGel™ Mini system (E1101; includes gel box, power supply, and casting sets)
• SmartDoc™ 2.0 Gel Viewing and Smart Phone Imaging System (E5001-SDB)
• SmartGlow DNA Stain, 1.0 mL (BE4500-PS or BE4500-LD)
• Agarose LE, 100 grams

PR1MA™ LB Broth 1 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

Now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. For propagation and maintenance of E.Coli. Low Salt Formula. Molecular Biology Grade.

Add 20 capsules per liter of water. Heat and stir until capsule is completely dissolved. Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.15% solution @25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ NZCYM Broth

Solubility: Water
Storage Temp: Room Temperature

Enriched medium for cultivating recombinant strains of E.Coli and propagating theta bacteriophage.

Dissolve 22 grams in 1 liter distilled water. Sterilize by autoclaving for 15 minutes.

Casein Digest Protein: 10 g/L
Yeast Extract: 5 g/L
Casamino Acids: 1 g/L
Magnesium Sulfate: 0.98 g/L
Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2.2 % Solution): 6.8 - 7.2
• Escherichia coli ATCC 23724: Good to Excellent
• Escherichia coli ATCC 33694: Good to Excellent
• Escherichia coli ATCC 33849: Good to Excellent
• Escherichia coli ATCC 39403: Good to Excellent
• Escherichia coli ATCC 47014: Good to Excellent
• Escherichia coli ATCC 53868: Good to Excellent
• Country of Origin: Report

PR1MA™ Nutrient Broth

Solubility: Water
Storage Temp: Room Temperature

For cultivating a variety of non-fastidious organisms.

Dissolve 8 grams in one liter distilled water. Heat solution to boiling with continuous stirring for one minute. Sterilize by autoclaving at 121°C for 15 minutes. Store at room temperature.

Beef Extract: 3 g/L
Pancreatic Digest of Gelatin: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous Free Flowing,Beige Powder
• pH (0.8 % Solution @25 ^oC): 6.6 - 7.0
• Microorganism (Bacillus Subtilis ATCC 9372): Fair to Good
• Microorganism (Escherichia Coli ATCC 25922): Good to Excellent
• Microorganism (Salmonella Typhimurium ATCC 14028 ): Fair to Excellent
• Microorganism (Staphylococcus Aureus ATCC 25923): Good to Excellent
• Pancreatic digest of gelatin: 5.0 g/L
• Beef Extract: 3.0 g/L

PR1MA™ Ethanol, Reagent Grade

Components:

• Ethyl Alcohol: 90%
• 2-Propanol: 5%
• Methanol: 5%

PR1MA™ Nutrient Agar

Solubility: Water with Heat

Storage Temp: Room Temperature

For cultivating a variety of non-fastidious organisms.

Dissolve 23 g in one liter of distilled water. Heat the solution to a boil with continuous stirring for one minute. Sterilize by autoclaving at 121°C for 15 minutes. Mix the medium with gentle agitation to avoid bubbles prior to pouring plates. Store at room temperature.

Ingredients

• Beef Extract: 3 g/L
• Pancreatic Digest of Gelatin: 5 g/L
• Shipping Dimensions: 3.50 x 7.00 x 3.50
• Agar: 15 g/L
• Shipping Weight: 1.2 lbs

*Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous Free Flowing, Beige
• PPM Appearance: Trace to Light Haze, None to Light PPT, Light Beige Amber
• pH (2.3% Solution @25 oC): 6.6 - 7.0
• Bacillus Subtilis ATCC 9372: Good to Excellent
• Microorganism (Escherichia Coli ATCC 25922): Good to Excellent
• Microorganism -Pseudomonas Aeruginosa ATCC 27853: Good to Excellent
• Microorganism (Staphylococcus Aureus ATCC 25923): Good to Excellent
• Salmonella Typhimurium ATCC 14028: Good to Excellent

PR1MA™ Luria Broth 1 Liter Buffered Capsules [Miller's LB Broth]

Each capsules has 25 g.  The 500 g package has 20 capsules, the 1 KG is 40 capsules and the 5 KG is 200 capsules.

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1; 1185-53-1
Solubility: Water
 Storage Temp: Room Temperature

Now available in convenient pre-measured and pre-mixed capsules. Pre-buffered media eliminates messy weighing and pH adjustments.

Simply dissolve 1 capsule per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.2 at 20°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.

For maintenance and propagation of E. Coli. High Salt Formula. Molecular Biology Grade.

Ingredients:

• Tryptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L
• Tris/Tris HCl: 1.5 g/L

Research or further manufacturing use only, not for food or drug use. 

• Appearance: Capsules (cream to pale beige free flowing powder)
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent