PR1MA™  SOB Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 7487-88-9; 7447-40-7
Solubility: Water
Storage Temp: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.

Add two capsules to one liter of distilled water. Heat and stir gently to completely dissolve medium. Sterilize by autoclaving at 121°C for 15 minutes.

Final Solution contains:

• Casein Enzymic Hydrolysate: 20 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 0.5 g/L
• Magnesium Sulfate: 2.4 g/L
• Potassium Chloride: 0.186 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH @25 °C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Citric Acid Anhydrous

CAS Number: 77-92-9
Molecular Weight: 192.1
Chemical Formula: C₆H₈O₇
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine white crystalline powder
• Assay (Titration): ≥ 99.5 %
• Carbonizable Substances (H₂SO₄): Pass Test
• Clarity and Color: Pass Test
• Identification: Pass Test
• Lead : ≤ 0.5 mg/kg
• Limit of Oxalic Acid: ≤ 0.036 %
• Oxalate Test: Pass Test
• Residual Solvents: Meet requirements
• Residue on Ignition: ≤ 0.05 %
• Sulfate: ≤ 0.015 %
• Water: ≤ 0.5 %

PR1MA™ LB Agar, Low Salt Formula, Powder [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Molecular Biology Grade. For propagation and maintenance of E. Coli. Low Salt Formula.

Dissolve 35 grams in 1 Liter of distilled water. Adjust the pH to 7.2 Sterilize by autoclaving for 15 minutes

Ingredients:

• Casein Peptone: 10 g / L
• Sodium Chloride: 5 g / L
• Yeast Extract: 5 g / L
• Agar: 15 g / L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• pH: 6.8 - 7.2
• Solubility: Clear Solution

PR1MA™ Yeast Nitrogen Base without Amino Acids

Solubility: Water
Storage Temp: Room Temperature

For classifying yeasts based on amino acid and carbohydrates requirements. Contains all essential vitamins and inorganic salts necessary for the cultivation of yeasts. Excludes histidine methionine, tryptophan and carbon source. Also contains <1 mg/L each of vitamins and trace inorganic salts.

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Free flowing powder, Homogeneous, Off white to cream
• PPM Appearance: Pale to light yellow (10X Solution)
• pH (0.67% Solution @25 ^oC): 5.2 - 5.6
• Microorganism Koleckera Apiculata ATCC 9774 (With Additives): Good to Excellent
• Microorganism Saccharomyces Cerevisiae ATCC 9080 (With Additives): Good to Excellent
• Microorganism Saccharomyces Cerevisiae ATCC 9763 (With Additives): Good to Excellent
• Microorganisms Candida Albicans ATCC 10231 (With Additives): Good to Excellent

PR1MA™  Lysozyme from Chicken Egg White, Salt Free

CAS Number: 12650-88-3
Solubility: Water
Storage Temp: 2-8°C

Purified from chicken egg white, 2x crystallized, dialyzed and supplied as a salt free lyophilized powder.

Dry powder stable for 3 - 5 years when stored dry at 2°C to 8°C.

Stock solution is stable up to 3 days at 4°C and up to 1 year at -20°C.

Research or further manufacturing use only, not for food or drug use.

• Appearance: 2X Crystallized; dialyzed, lyophilized powder
• A 280@ mg/ml: Report
• Protein: Report %
• U/mg dw (Unit Per mg Dry Weight): ≥ 8000
• Raw Material Species: Chicken Egg Whites
• Country of Origin: Report

PR1MA™  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g / L
• Sodium Chloride: 10 g / L
• Yeast Extract: 5 g / L
• Agar: 12 g / L

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, none to light ppt, light yellow
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2% water) @25°C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Casein Peptone: 10 g/L

PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant proteins. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.

Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™  PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution]

Common Name: Phosphate Buffered Saline 1X Sterile Solution
CAS Number: 7647-14-5, 7447-40-7, 7558-79-4, 7778-77-0, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.

Ready to use sterile 1X solution.

Ingredients:

• Sodium Chloride: 137 mM
• Potassium Chloride: 2.7 mM
• Phosphate Buffer: 11.9 mM
• pH: 7.4
• Does not contain Calcium or Magnesium

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Solution
• pH: 7.25 - 7.55

PR1MA™ Terrific Broth Modified Capsules

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Terrific Broth formulation packaged in easy to use 24 gram capsules. Formulation from powder.

Add 1 capsule per 500 mL of water. Heat and stir until capsules are completely dissolved. Add 2ml of glycerol to mixture. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Casein Digest Peptone: 12 g/L
• Yeast Extract: 24 g/L
• Dipotassium Phosphate: 9.4 g/L
• Potassium Phosphate: 2.2 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Water soluble gelatin capsules containing cream to beige free flowing powder.
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH (4.76% solution at 25°C): 7.0 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Yeast Extract: 24.0 g/L
• Casein Peptone: 12.0 g/L
• Potassium phosphate dibasic: 9.4 g/L
• Potassium phosphate monobasic: 2.2 g/L
• Direction (Preparation): Add 2 capsules to one liter of purified water until evenly dispersed. Add 4ml of Glycerol to the mixture. Boil for one minute with stirring to completely dissolve. Distribute and autoclave at 121°C for 15 minutes.

PR1MA™ LB Broth 20 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

Now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. Low Salt Formula. Molecular Biology Grade.

For propagation and maintenance of E.Coli:

• Add 1 capsule per liter of water.
• Heat and stir until capsule is completely dissolved.
• Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl
• Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Nematode Growth Medium, Powder

Common Name: Nematode Growth Medium

Solubility: Water with Heat

Storage Temp: Room Temperature

A standard formulation for use in Nematode growth studies.

Contains:

Agar 17.5 g/l
Sodium Chloride 3.0 g/l
Peptone 2.5 g/l
Cholesterol 0.005 g/l
Shipping Dimensions: 3.50 x 3.75 x 3.50
Shipping Weight: 1.2 lbs

Research or further manufacturing use only, not for food or drug use. 

Specifications

• Appearance: Light tan to pale yellow powder
• Solubility: Clear Solution
• pH (2.3% Solution @25 oC): Report
• Agar: 17.5 g/l
• Sodium Chloride: 3.0 g/l
• Peptone: 2.5 g/l
• Cholesterol: 0.005 g/l