For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® HEK293
 
Ready-to-use, xeno-free (animal origin-free) hydrogel system for 3D culture of HEK293 cells

VitroGel® HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells.

• Simple 5-minute protocol
• High-quality 3D spheroids
• Easily scale up with flasks and bioreactors
• Easy cell harvesting protocol (Enzyme-free, No trypsin)

VitroGel® HEK293 is a unique system to easily support the rapid 3D growth of HEK293 cells.  Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D static suspension cultures. Both our standard 3D cell culture protocol and the 3D static suspension culture protocols can be used for rapid cell expansion. The high-quality 3D spheroids enhance the cell performance for protein expression and downstream applications. HEK293 cells cultured in VitroGel® HEK293 can easily be scale-up for large-scale bioreactors or spin flasks. By using the VitroGel® Cell Recovery Solution, the cell harvesting after 3D culture is simple and effective.

Specifications 

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

GelNest™ Matrix

GelNest™ Matrix is derived from mouse tumor tissue and contains extracellular matrix components such as laminin, type IV collagen, heparan sulfate proteoglycans, and more. These components support cell adhesion, differentiation, and proliferation, providing signals for these processes. Additionally, they simulate the characteristics of the basement membrane in the physiological environment, enhancing the success rate and effectiveness of cell culture.

In addition to the matrix components, GelNest™ Matrix is rich in various growth factors. These growth factors promote cell differentiation, proliferation, and migration, mimicking cellular signaling pathways and interactions in the physiological environment. GelNest™ Matrix has a wide range of applications, particularly in tissue engineering, cell culture, and research. It can be used for organoid culture, stem cell differentiation, angiogenesis, migration or invasion assays, and in vivo tumor studies.

Storage and Operation Instructions

To store GelNest™ Matrix, it is recommended to keep it in a -20°C freezer until ready for use. Prior to initial use, thaw the gel and divide it into single-use portions. Store these portions in a -80°C freezer for a maximum of 2 years. Please note that GelNest™ Matrix Gel should not be stored in a frost-free freezer.

GelNest™ Matrix is in a liquid state at 4°C and transitions into a gel state at 37°C. It starts to solidify into a gel when the temperature exceeds 10°C. For optimal performance, it is advised to pre-chill the pipette tips and conduct the procedures on ice.

GelNest™ Matrix Quality Assurance

• The protein concentration is guaranteed to be between 8 - 20 mg / mL
• The gel formation performance remains stable
• There are no LDEV (Lactate Dehydrogenase Elevated Virus), bacteria, or mycoplasma present for a high safety performance
• The endotoxin level is < 10 EU / mL
• Tests for organ-like structures and stem cell culture have been successfully performed.
• Tests for angiogenesis, tumor invasion, and tumor formation have been successfully conducted 

Specifications
 

Item	Growth Factor	Phenol Red	Recommended Applications	Volume
211212	Normal	Yes	General 2D, 3D cell culture	5 mL
211222	Normal	No	Requires colorimetric identification (such as fluorescence) or sensitivity to steroids	5 mL
211232	Low	Yes	2D, 3D cell culture with higher accuracy requirements for matrix components and requires	5 mL
211242	Low	No	Colorimetric identification or sensitivity to steroids	5 mL
211252	Normal	Yes	In vivo tumor formation, thrombosis test, angiogenesis experiment, general cell culture, etc.	5 mL
211262	Normal	No	2D, 3D cell culture with higher accuracy requirements for matrix components	5 mL
211272	Low	Yes	hESC stem cell culture	5 mL
211282	Low	No	Organoid culture and differentiation	5 mL
211492	Normal	Yes	Angiogenesis Experiment	5 mL

Sodium Phosphate Dibasic, Anhydrous

• Appearance: White Crystalline Powder
• Purity: ≥ 98.0%
• pH (1% Aqueous): 8.4 – 9.6
• Solubility: Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C / 4h): ≤ 0.5%
• Insoluble Matter: ≤ 0.2%
• Phosphate: 49.0 - 51.0%
• Fluoride: ≤ 10 ppm
• Arsenic: ≤ 1 ppm
• Lead: ≤ 1 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs

ION Ethidium Homodimer I

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Hydrogel Matrix
 
ready-to-use, xeno-free (animal origin-free) functional hydrogel system

• Xeno-free, biological functional hydrogel
• Ready-to-use at room temperature (20 min protocol)
• Supports a wide range of cell types and applications
• Easy, efficient, and enzyme-free cell harvesting in 20 min

VitroGel® Hydrogel Matrix is a ready-to-use, xeno-free (animal origin-free) functional hydrogel for 3D cell culture research. VitroGel® Hydrogel Matrix is an optimized formulation of multi-functional ligands and concentration to support a wide range of cell types for different applications.“Just Add Cells” – The hydrogel matrix is ready to mix with cell suspension directly.

There is no additional adjustment needed. VitroGel® Hydrogel Matrix closely mimics the natural extracellular matrix (ECM) environment to make cells feel more like at home. The hydrogel is room temperature stable, has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. This user-friendly functional hydrogel creates an excellent balance of simplicity and versatility.

3D cell culture process in 20 min – “Just add cells”

VitroGel® Hydrogel Matrix is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.

Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

Sodium Phosphate Monobasic, Monohydrate

• Appearance (Color): White to Off-White
• Appearance (Form): Crystalline Powder
• Purity: ≥ 99.0%
• pH (5% Aqueous): 8.7 - 9.3
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4h): 10.0 - 15.0%
• Insoluble Matter: ≤ 0.2%
• Lead: ≤ 4 ppm
• Arsenic: ≤ 3 ppm
• Phosphorous: 20.0 - 24.0%
• Chloride: ≤ 140 ppm
• Fluoride: ≤ 50 ppm
• Sulfate: ≤ 150 ppm
• RNase: None Detected
• DNase: None Detected 

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Western Lightning Plus (NEL103):
·  Twice the sensitivity of standard products 

·  Direct protocol transfer 

·  ~ 1 picogram sensitivity

·  > 2 hr signal duration for re-exposing film

·  Working solution stability 8 hr at room temperature 

·  PVDF or Nitrocellulose membranes 

·  Non-toxic (no msds required)

Western Lightning Pro (NEL120)
·  Mid-range sensitivity, with broad dynamic range

·  Detection of less than 1 picogram amount of protein

·  Long, stable signal > 12 hr for re-exposing film 

·  Stable at room temperature >1 year 

·  Working solution stable for > 24 hr at room temperature

·  Non-toxic (no msds required)