Chemicals

MIDSCI is Your Trusted Source for High-Quality Lab Chemicals and Reagents

Looking for reliable chemicals to power your research? MIDSCI delivers a full range of high-purity reagents, solvents, and buffers designed for life science, pharmaceutical, microbiology, and organic chemistry workflows. From essential acids and bases to specialized biochemicals, growth media, and DNA kits, our inventory ensures precision, consistency, and compliance, backed by Certificates of Analysis (COAs) and Safety Data Sheets (SDS).

Explore PR1MA Chemicals, our premium line of USP- and ACS-grade chemicals

Perfect for molecular biology, plant science, and cellular research, PR1MA products offer full traceability and documentation for your peace of mind.

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Thallos AM
 
Best in class, green fluorescent, thallium (Tl+) indicator, membrane permeable. Works with potassium (K+), sodium (Na+), and monovalent cation channels, and transporters.
 
Thallos is a green fluorescent, intracellular thallium (Tl+) indicator, and has been the gold standard for fluorescence-based potassium (K+) channel HTS for nearly 2 decades. Thallos also delivers outstanding results for a wide variety of monovalent cation (sodium – Na+) channels, transporters, and GPCRs.
Discontinued, Contact us for more options!

Western Blot 2.0 with PURITY


The PURITY detection system revolutionizes Western Blotting in three steps.
 

Blocking buffer and secondary antibody are already components of the PURITY Reagent. And the washing buffer is also included. The only thing to add is your specific primary antibody.


Benefits:

  • 1 simple protocol which makes almost all optimization unnecessary.
  • Increased sensitivity and reproducibility of blots.
  • Dramatically reduces the number of steps and time required for Western blots.
  • Drastically decreases background phenomena.
  • Highly reliable.
Item#:
ASWESTBLOTACC

ION Biosciences Fluo-2 K+ Salt

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable. Higher Ca²+ affinity than Fluo-4.

Fluo-2 is a green fluorescent, calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.

Fluo-2 K+ salt is a membrane impermeable form of Fluo-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ION Biosciences Fura-2 LR K+ Salt
 
Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane impermeable.
 
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
 
Fura-2 LR K+ salt is a membrane impermeable form of Fura-2 LR that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
ION Biosciences BCECF AM

BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings.
ION Biosciences Brilliant Sodium Flex Kit
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
ION Biosciences Essentials Red Calcium

Red Calcium Essentials provides the necessary reagents for multi-well plate-based, fluorescence microscopy applications and high-throughput measurements of intracellular calcium (Ca²+) mediated changes through a wide variety of plasma membrane and intracellular calcium channels and transporters. Red Calcium Essentials is also useful for investigating numerous effectors of ion channels and transporters, including G-protein coupled receptors.

Our red calcium dye, ION Calcium Red-1 (ICR-1), is a red fluorescent indicator (Ex/Em: 580/660 nm) responsive to intracellular Ca²+ dynamics. ICR-1’s spectral properties allows for multiplexing with an indicator of another color or GFP-tagged cells.

When following the recommended protocol, Red Calcium Essentials provides enough reagents to make 100 mL of working solution, enough for ten 96-well plates. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASESSREDCAL

PR1MA  TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C4H11NO3  HCl
Solubility: Water
Storage Temp: Room Temperature

Highly purified for use in molecular biology applications.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline
  • Assay: 99.0 - 101.0 %
  • pH (1% aqueous solution): 4.2 - 5.0
  • Solubility (1M Solution in Water ) : Clear, colorless solution
  • UV Absorbance @260nm, 1.0 M in H2O:  0.06
  • UV Absorbance @280nm, 1.0 M in H2O:  0.05
  • Identification: Conforms
  • Heavy Metals :  5 ppm
  • Grade: USP

  

PR1MA Nystatin

CAS Number: 1400-61-9
Molecular Weight: 926.1
Chemical Formula: C47H75NO17
Solubility: DMSO
Storage Temp: 2-8°C

Polyene antifungal antibiotic produced by Streptomyces noursei.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Yellow to light tan powder
  • Crystallinity: Pass Test
  • Identification: Pass Test
  • Loss on Drying:  5.0 %
  • pH @25 °C: 6.0 - 8.0
  • Potency (Assay):  4400 U/mg
  • Suspendability: Pass Test
  • Residual Solvent (n-butanol):  1000 ppm
  • Residual Solvent (Methanol):  3000 ppm
  • Residual Solvent (Acetone):  5000 ppm
  • Residual Solvents: The following Class 1,2, or 3 Solvents may be present in this product. All comply with the limits prescribed in the current USP.

  

PR1MA  MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid]

Common Name: 2-(N-Morpholino)ethanesulfonic acid
CAS Number: 145224-94-8
Molecular Weight: 213.24
Chemical Formula: C6H13NO4 H 2O
Solubility: Water
Storage Temp: Room Temperature

Buffer for use in plant tissue culture.

  • pKa (20°C): 6.15
  • pH range: 5.5 - 6.7

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Assay (Titration):  99.0 %
  • Heavy Metals:  0.0010 %
  • Loss on Drying: 7.0 - 10.0 %
  • pH: 2.5 - 4.0
  • pKa @20 oC: 6.02 - 6.25
  • Solubility (10% solution in H2O): Clear
  • Sulfate:  0.01 %
  • UV Absorbance 250nm in 10% H2O:  0.05
  • Country of Origin: Report

  

PR1MA Potassium Acetate

CAS Number: 127-08-2
Molecular Weight: 98.14
Chemical Formula: C2H3O2K
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Calcium (Ca):  0.005 %
  • Chloride (Cl):  0.003 %
  • Heavy Metals :  5 ppm
  • Insolubles:  0.005 %
  • Iron:  5 ppm
  • Magnesium (Mg):  0.002 %
  • pH (5% in water): 6.5 - 9.0
  • Phosphate:  0.001 %
  • Purity:  99.0 %
  • Sodium (Na):  0.03 %
  • Sulfate:  0.002 %
  • Country of Origin: Report

  


PR1MA  Rifampicin

CAS Number: 13292-46-1
Molecular Weight: 823.0
Chemical Formula: C43H58N4O12
Solubility: Dilute acid solutions
Storage Temp: 2-8°C

Interferes with synthesis of nucleic acids by inhibiting DNA dependent RNA polymerase. Active against gram positive bacteria. Less active against gram negative bacteria. Plant cell culture tested.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Brownish-Red Crystalline Powder
  • Crystallinity: Pass Test
  • Heavy Metals :  20 ppm
  • Identification: Pass Test
  • Loss on Drying:  1.0 %
  • pH: 4.5 - 6.5
  • Purity: 97.0 - 102.0 %
  • Rifampicine Quinone:  1.5 %
  • Solubility: Pass
  • Sulfated Ash:  0.1 %

  

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