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TheWell Bioscience VitroGel® 3D High Concentration

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® 3D High Concentration

tunable, xeno-free hydrogel, high concentration (3 mL kit)

Dilution Solution TYPE 1 contains sucrose for best osmolarity
Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® 3D High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system that allows the maximum flexibility to manipulate the 3D cell culture environment for different needs. VitroGel 3D High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa. The tunability of the hydrogel gives the ability for researchers to create an optimized environment for cell growth. The VitroGel 3D High Concentration hydrogel matrix structure is good for cell spheroid formation, suspension cells, or cells requiring low cell-matrix interactions.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications

Contents	VitroGel® 3D High Concentration, 3 mL
Contents	VitroGel® Dilution Solution, 50 mL
Hydrogel Formulation	Xeno-free tunable hydrogel, pure and unmodified.
Hydrogel Formulation	HIGH CONCENTRATION
Use	Good for cell spheroid formation, suspension cells or cells require low cell-matrix interactions
Operation	Room temperature
Hydrogel Strength	10 to 4,000 Pa of G’ depending on dilution ratio.
Hydrogel Strength	Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.
pH	Neutral
Color	Transparent
Cell Harvesting	20 min cell recovery using VitroGel Cell Recovery Solution
Injectable	Injectable hydrogel
Storage	Store at 2-8°C. Ships at ambient temperature
Number of Uses	Dilution ratio:
	1:2 = 225 uses at 50 µL per well
	1:3 = 300 uses at 50 µL per well
	1:5 = 450 uses at 50 µL per well

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

Item#:

ASVITRO3D

FAQ document - Xenograft

VitroGel® Sale Sheet

VitroGel® Safety Data Sheet

Xenograft Applications of VitroGel

TheWell Bioscience VitroGel® Cell Recovery Solution (100 mL)

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Cell Recovery Solution (100 mL)

Enzyme-free cell harvesting solution to recover cells from the hydrogel in 20 min.

VitroGel® Cell Recovery Solution is an enzyme-free, ready-to-use solution to harvest 2D, 3D, and organoids cultured cells efficiently and safely. The solution can recover cells from VitroGel® hydrogels in 20 minutes.

VitroGel® Cell Recovery Solution is room temperature stable, has a neutral pH, and works at 37°C operating temperature. The solution can maintain high cell viability during the recovery process. Harvested cells can be sub-culture in both 2D and 3D cultures.

The VitroGel® Cell Recovery Solution can be used before or after the fixation and stained preparation of hydrogel specimens to ensure high-quality downstream data analysis.

Specifications

Size	100 mL
Formulation	Enzyme-free
Use	Harvest cells from VitroGel® hydrogel while maintaining high cell viability.
Use	Use before or after sample fixation and stained preparation for imaging or downstream data analysis
Processing Time	15-20 min
Downstream	Recovered cells can be sub-culture in both 2D and 3D culture
pH	Neutral
Storage	Ambient Temperature (15-30°C)

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

Item#:

ASVITROCRS

FAQ document - Xenograft

VitroGel® Dilution Solution Safety Data Sheet

VitroGel® Hydrogel High-Concentration Handbook

Xenograft Applications of VitroGel

TheWell Bioscience VitroGel® IKVAV High Concentration

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® IKVAV High Concentration

Laminin-derived functional peptide (IKVAV) modified – tunable, xeno-free hydrogel, high concentration (3 mL kit)

Dilution Solution TYPE 1 contains sucrose for best osmolarity
Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® IKVAV High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (IKVAV). IKVAV is the bioactive sequence located on the C-terminal end of the long arm of the α-1 chain of laminin, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth. VitroGel® IKVAV High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications

Contents	VitroGel® RGD High Concentration, 3 mL
Contents	VitroGel® Dilution Solution, 50 mL
Hydrogel Formulation	Xeno-free tunable hydrogel modified with RGD peptide.
Hydrogel Formulation	HIGH CONCENTRATION
Use	Good for adhesion cells or cells requiring stronger cell-matrix interactions.
Mix & Match	Can be blended with other versions of VitroGel® to create a multi-functional hydrogel
Operation	Room temperature
Hydrogel Strength	10 to 4,000 Pa of G’ depending on dilution ratio.
Hydrogel Strength	Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.
pH	Neutral
Color	Transparent
Cell Harvesting	20 min cell recovery using VitroGel® Cell Recovery Solution
Injectable	Injectable hydrogel
Storage	Store at 2-8°C. Ships at ambient temperature
Number of Uses	Dilution ratio:
	1:2 = 225 uses at 50 µL per well
	1:3 = 300 uses at 50 µL per well
	1:5 = 450 uses at 50 µL per well

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

Item#:

ASVITROIKVAV

FAQ document - Xenograft

VitroGel® Sale Sheet

VitroGel® Safety Data Sheet

Xenograft Applications of VitroGel

ION Biosciences Fura-2 K+ Salt

ION Biosciences Fura-2 K+ Salt

Ratiometric calcium (Ca²+) indicator, membrane impermeable.

Fura-2 is the most popular UV-excitable, ratiometric green indicator for calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Fura-2 K+ salt is a membrane impermeable form of Fura-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Item#:

ASFURA2KST

Fura-2 K+ Salt Safety Data Sheet

Fura-2 K+ Salt Product Specifications

TheWell Bioscience VitroGel® YIGSR High Concentration

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® YIGSR High Concentration

Laminin-derived functional peptide (YIGSR) modified – tunable, xeno-free hydrogel, high concentration (3 mL kit)

Dilution Solution TYPE 1 contains sucrose for best osmolarity
Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® YIGSR High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (YIGSR). YIGSR sequence resides on the laminin β1 chain, which involves endothelial cell adhesion, cell proliferation and motility/migration. VitroGel® YIGSR High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications

Contents	VitroGel® YIGSR High Concentration, 3 mL
Contents	VitroGel® Dilution Solution, 50 mL
Hydrogel Formulation	Xeno-free tunable hydrogel modified with YIGSR peptide. HIGH CONCENTRATION
Use	Support endothelial cell adhesion, cell proliferation and motility/migration
Mix & Match	Can be blended with other versions of VitroGel® to create a multi-functional hydrogel
Operation	Room temperature
Hydrogel Strength	10 to 4,000 Pa of G’ depending on dilution ratio.
Hydrogel Strength	Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.
pH	Neutral
Color	Transparent
Cell Harvesting	20 min cell recovery using VitroGel® Cell Recovery Solution
Injectable	Injectable hydrogel
Storage	Store at 2-8°C. Ships at ambient temperature
Number of Uses	Dilution ratio:
	1:2 = 225 uses at 50 µL per well
	1:3 = 300 uses at 50 µL per well
	1:5 = 450 uses at 50 µL per well

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans. To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

Item#:

ASVITROYIGSR

FAQ document - Xenograft

VitroGel® Sale Sheet

VitroGel® Safety Data Sheet

Xenograft Applications of VitroGel

TheWell Bioscience VitroGel® COL High Concentration

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® COL High Concentration

collagen-mimetic functional hydrogel – tunable, xeno-free (3 mL)

Dilution Solution TYPE 1 contains sucrose for best osmolarity
Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo, which shows great potential for tissue engineering and regeneration medicine application. VitroGel® COL High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications

Contents	VitroGel® COL High Concentration, 3 mL
Contents	VitroGel® Dilution Solution, 50 mL
Hydrogel Formulation	Xeno-free tunable collagen-mimetic functional hydrogel. HIGH CONCENTRATION
Use	Support integrin binding to promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo
Mix & Match	Can be blended with other versions of VitroGel to create a multi-functional hydrogel
Operation	Room temperature
Hydrogel Strength	10 to 4,000 Pa of G’ depending on dilution ratio.
Hydrogel Strength	Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.
pH	Neutral
Color	Transparent
Cell Harvesting	20 min cell recovery using VitroGel Cell Recovery Solution
Injectable	Injectable hydrogel
Storage	Store at 2-8°C. Ships at ambient temperature
Number of Uses	Dilution ratio:
	1:2 = 225 uses at 50 µL per well
	1:3 = 300 uses at 50 µL per well
	1:5 = 450 uses at 50 µL per well

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

Item#:

ASVITROCOL

FAQ document - Xenograft

VitroGel® Sale Sheet

VitroGel® Safety Data Sheet

Xenograft Applications of VitroGel

ION Biosciences ION Vital - MitoVolt Kit

ION VITAL – MitoVolt

Mitochondrial membrane potential assay kit that uses JC-10, a membrane potential sensitive dye that is more soluble than JC-1.

ION Vital – MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. ION Vital – MitoVolt is compatible with fluorescence microscopy, flow cytometry, and plate reader applications.

JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence.
JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.

ION Vital – MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, ION Vital – MitoVolt provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application.

Item#:

ASVITALMITOVOLT

ION Vital - MitoVolt Kit Safety Data Sheet

ION VITAL MITOVOLT PROTOCOL

ION Biosciences Fura-2 AM

ION Biosciences Fura-2 AM

UV-excitable, ratiometric green calcium (Ca²+) indicator, membrane permeable.

Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Item#:

ASFURA2AM

FURA 2 AM PROTOCOL

Fura-2 AM Safety Data Sheet

Fura-2 AM Product Specifications

ION Biosciences ING-2 TMA+ Salt

ION Biosciences ING-2 TMA+ Salt

Yellow-green fluorescent, sodium (Na+) indicator, membrane impermeable. Higher Na+ affinity than ING-1.

ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na+) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na+ indicator for high-throughput screening applications targeting Na+ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.

ING-2 TMA+ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced

Item#:

ASIGN2TMAST

ING-2 TMA+ Salt Safety Data Sheet

ING-2 TMA+ Salt Product Specifications

ION Biosciences Brilliant Calcium Kits

Brilliant Calcium Kits

High-throughput, no wash calcium (Ca²+) assay. An optimal solution for measuring intracellular Ca²+ dynamics, including effectors such as GPCRs and protein transporters.

ION Biosciences’ Brilliant Calcium (Ca²+) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²+. The ION Biosciences’ Brilliant Calcium Assay is useful for a wide range of effectors of intracellular Ca²+ including many G protein-coupled receptors (GPCRs), divalent cation channels, and other proteins capable of transporting Ca²+ or affecting intracellular Ca²+ dynamics. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca²+ levels.

Brilliant Calcium Flex Kit

Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.

Contents of Brilliant Calcium Flex Kit

Label	Name	Volume	Containers	Storage
Reagent A	Brilliant Calcium Reagent	Dry	10	-20° C
Reagent B	DMSO	225 μL	1	4° C
Reagent C	DySolv	4 mL	1	4° C
Reagent D	10X Assay Buffer	20 mL	1	4° C
Reagent E	TRS	4 mL	1	4° C
Reagent F	Probenecid Solution	4 mL	1	4° C

Brilliant Calcium Express Kit

Our Express kit is custom tailored to customer’s needs, providing only the reagents you need in the amounts you need them. Less waste, higher reproducibility, and more convenience.

*Research or further manufacturing use only, not for food or drug use.

Item#:

ASBRILLIANTCAL

Brilliant Calcium Flex Sample Kit Instructions

BRILLIANT CALCIUM FLEX PROTOCOL

ION Biosciences IPG-2 AM

ION Biosciences IPG-2 AM

Yellow-green fluorescent, potassium (K+) indicator, membrane permeable.

Lower K+ affinity than IPG-4 and higher affinity than IPG-1

ION Potassium Green – 2 (IPG-2) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Kd = 50 mM) and lower affinity than IPG-4 (Kd = 7 mM).

Item#:

ASIPG2AM

IPG 2 AM PROTOCOL

IPG-2 AM Safety Data Sheet

IPG-2 AM Product Specifications

ION Biosciences Fluo-3 K+ Salt

ION Biosciences Fluo-3 K+ Salt

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable

Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications.Is preferred over Fluo-4 for some cell types.

Fluo-3 K+ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Item#:

ASFLUO3AMST

Fluo-3 K+ Salt Safety Data Sheet

Fluo-3 K+ Salt Product Specifications

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