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Chemicals
MIDSCI™ is Your Trusted Source for High-Quality Lab Chemicals and Reagents
Looking for reliable chemicals to power your research? MIDSCI™ delivers a full range of high-purity reagents, solvents, and buffers designed for life science, pharmaceutical, microbiology, and organic chemistry workflows. From essential acids and bases to specialized biochemicals, growth media, and DNA kits, our inventory ensures precision, consistency, and compliance, backed by Certificates of Analysis (COAs) and Safety Data Sheets (SDS).
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Perkin Elmer's ATPlite simple and sensitive system is an excellent kit to determine cell number due to proliferation or toxicity. It effectively replaces the MTT assay and can be used instead of the 3H thymidine assays with additional analysis.
- Choose ATPlite (2 steps):
- Half-life> 4 hours
- Longest half - life = for batch processing of samples
- 2 step system with separate lysis and detection steps
- More suited for Academia Choose
- ATPlite 1step:
- Measure within 30 min
- Brightest signal (~3x the counts of ATPlite 2Steps) = when looking for maximum sensitivity
- For continuous processing of samples
- True 1 step system = More suited to HTS
6016736 |
6016731 |
6016739 |
6016943 |
6016941 |
6016947 |
6016949 |
|
| Assay Protocol | 1-step |
1-step |
1-step |
2-step |
2-step |
2-step |
2-step |
| Automation Compatible | Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
| Detection Method | Luminescence |
Luminescence |
Luminescence |
Luminescence |
Luminescence |
Luminescence |
Luminescence |
| Experimental Type | In vitro |
In vitro |
In vitro |
In vitro |
In vitro |
In vitro |
In vitro |
| One Unit Contains | 10 mL |
100 mL |
1000 mL |
300 assays |
1000 assays |
5000 assays |
10000 assays |
| Product Brand Name | ATPlite |
ATPlite |
ATPlite |
ATPlite |
ATPlite |
ATPlite |
ATPlite |
| Shipping Condition | Ambient |
Ambient |
Ambient |
Ambient |
Ambient |
Ambient |
Ambient |
| Unit Size | 100 (96-well) / 400 (384-well) |
1,000 (96-well) / 4,000 (384-well) |
10,000 (96-well) / 40,000 (384-well) |
300 (96-well) / 1200 (384-well) |
1,000 assay points |
5,000 (96-well) / 20,000 (384-well) |
10,000 (96-well) / 40,000 (384-well) |
| Vol Capacity | 10 mL |
100 mL |
1000 mL |
N/A |
N/A |
N/A |
N/A |
Item#:
ASLUMASSAY3
Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.
- Choose britelite plus:
- Half-life = 30 min
- Brightest signal = when looking for maximum sensitivity
- Shortest half-life = for continuous processing of samples
- Choose neolite:
- Half-life = 150 min
- High signal intensity, longer lasting signal = good balance
- For both continuous and batch processing of samples
- Best robustness (less dependent on mixing quality)
- Less sensitive to phenol red
- Can be stored 1 month at 22°C
- Choose steadylite plus:
- Half-life = 240 min
- Longest signal half-life = for batch processing of samples
- Super-sensitivity not needed
Item#:
ASLUMASSAY2
ION Biosciences Fura-2 LR AM
Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane permeable.
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
ION Biosciences ICR-1 AM
Red fluorescent, calcium (Ca²+) indicator, membrane permeable. Optimal for multiplexing and tissue imaging. Fluorescence lifetime and multiphoton compatible.
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²+) indicator for intracellular Ca²+ measurements.
ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
Item#:
ASICR1AM
ION Biosciences Fluo-4 AM
Green fluorescent, calcium (Ca²+) indicator, membrane permeable.
Fluo-4 is the most popular green fluorescent, intracellular calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.
Item#:
ASFLUO4AM
Calcein AM
Green fluorescent, live cell indicator.
Calcein AM is a membrane-permeant, non-fluorescent form of calcein that is converted to green fluorescent calcein in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein is well retained within the cytosol of most healthy cells with intact cell membranes.
Item#:
ASCALCEINTR
Pall® Ampoule
Media for Microbiological Analysis
- Sterile Ampoule Media Pre-Measured for Maximum Efficiency and Convenience
- Meets Membrane Filter (MF) Technique Standard Method requirements with 2 mL ampoules*
- Maximizes efficiency with premixed and presterilized media
- Choose between plastic or glass ampoules for your applications
- Simplify pouring of the media with wide-mouth glass ampoules
- All plastic ampoule media is economically packaged with 50 ampoules per box
Item#:
ASAMPOULEMEDIA
Sodium Chloride
- Appearance: White Crystalline Powder
- Purity: 99.0 - 101.5%
- pH (5% Aqueous): 5.0 - 9.0
- Lead: ≤ 5 ppm
- Iron: ≤ 2 ppm
- Insoluble Matter: ≤ 0.005%
- Iodide: ≤ 20 ppm
- Bromide: ≤ 100 ppm
- Phosphate: ≤ 5 ppm
- Sulfate: ≤ 40 ppm
- Barium: To Pass Test
- Calcium: ≤ 20 ppm
- Magnesium: ≤ 10 ppm
- Potassium: ≤ 50 ppm
- Chlorate & Nitrate: ≤ 30 ppm
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
Item#:
ASCHLORIDESOD1
MDR Activity Kit
ION’s Multidrug Resistance (MDR) Activity kit is an effective solution for detecting MDR1 and MRP1 activity and compounds susceptible to MDR–mediated efflux. ION’s MDR Activity kit is compatible with fluorescence microscopy, flow cytometry, and fluorescence plate readers using FITC/fluorescein settings.
Calcein AM is a membrane-permeant, non-fluorescent dye that enters cells passively. Once inside the cytosol of cells, intracellular esterases convert it to fluorescent Calcein (Ex/Em: 495 nm/515 nm), resulting in uniform cytosolic fluorescence. Drug efflux transporters, such as P-glycoprotein (Pgp, MDR1) and multidrug-resistance-associated protein (MRP1), actively extrude Calcein AM from inside the cell before esterases can convert it to Calcein. The presence of additional MDR substrates or inhibitors of MDR expression results in decreased Calcein AM efflux, causing a measurable increase in intracellular fluorescence. In addition to identifying MDR substrates and inhibitors, this kit can also be used to evaluate the activity of MDR transporters in cells.
ION’s Multidrug Resistance (MDR) Activity kit is an effective solution for detecting MDR1 and MRP1 activity and compounds susceptible to MDR–mediated efflux. ION’s MDR Activity kit is compatible with fluorescence microscopy, flow cytometry, and fluorescence plate readers using FITC/fluorescein settings.
Calcein AM is a membrane-permeant, non-fluorescent dye that enters cells passively. Once inside the cytosol of cells, intracellular esterases convert it to fluorescent Calcein (Ex/Em: 495 nm/515 nm), resulting in uniform cytosolic fluorescence. Drug efflux transporters, such as P-glycoprotein (Pgp, MDR1) and multidrug-resistance-associated protein (MRP1), actively extrude Calcein AM from inside the cell before esterases can convert it to Calcein. The presence of additional MDR substrates or inhibitors of MDR expression results in decreased Calcein AM efflux, causing a measurable increase in intracellular fluorescence. In addition to identifying MDR substrates and inhibitors, this kit can also be used to evaluate the activity of MDR transporters in cells.
When following our protocol, ION’s MDR Activity kit provides enough reagents to make 100 mL of working solution, enough for ten 96- or 384-well plates or 80 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASMDRACTKIT
ION Biosciences RATIOMETRIC CALCIUM ESSENTIALS
Ratiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca2+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2.
Ratiometry is not only optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, it also reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Item#:
ASESSRATIOCA
ION Biosciences Fluo-3 AM
Green fluorescent, calcium (Ca²+) indicator, membrane permeable.
Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.
ION Biosciences IPG-1 AM
Yellow-green fluorescent, potassium (K+) indicator, membrane permeable.
Lower K+ affinity than IPG-2 and IPG-4.
ION Potassium Green – 1 (IPG-1) is the best in class yellow-green fluorescent, intracellular potassium (K+) indicator for measuring changes at most intracellular K+ concentrations. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Kd = 18 mM) or IPG-4 (Kd = 7 mM).
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