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Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Sodium Phosphate Dibasic, Heptahydrate
- Appearance: White to Off-White Powder
- Purity: ≥ 99.0%
- pH (5% Aqueous) : 8.7 - 9.3
- Solubility: Clear and Colorless
- Identity (FTIR): Conforms to Structure
- Loss on Drying (105°C/4 h): 44 - 50%
- Lead: ≤ 10 ppm
- Iron: ≤ 10 ppm
- Chloride: ≤ 10 ppm
- Phosphate: 35.0% - 39.-%
- Sulfate: ≤ 50 ppm
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® ORGANOID
ready-to-use, xeno-free (animal origin-free) hydrogel system
for organoid culture
- Xeno-free hydrogel for organoid formation
- Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
- Scalable protocol for lab automation
- Easy 20-minute cell harvesting (enzyme-free)
- VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.
VitroGel® ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel® ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.
The VitroGel® ORGANOID hydrogels can work in conjunction with VitroGel® STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel® STEM to VitroGel® ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B. Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. VitroGel® ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.
Which organoid hydrogel to choose
We still strongly suggest starting with the VitroGel®
ORGANOID Discovery Kit (Item # VHM04-K) to make a quick screening to find the
hydrogel version that is optimal for the organoid conditions.
The kit includes all four types of organoid hydrogels:
VitroGel® ORGANOID-1, VitroGel® ORGANOID-2, VitroGel® ORGANOID-3, VitroGel® ORGANOID-4)
Each of the four types has various bio-functional ligands,
mechanical strengths, and degradability to fulfill the needs of different
organoid culture conditions.
Because there are a wide range of organoid cell resources
(from stem cells, patient-derived tissue, co-culture, to PDX), it is hard to
tell which organoid hydrogel would best perform the researcher’s experiment.
Therefore, the Discovery Kit helps researchers to screen the four different
formulations to determine the best organoid hydrogel version for the organoid
conditions.
From our findings, versions 1, 2, or 3 are suitable for
gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2
or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for
cancer organoids.
Specifications
|
Formulation |
Xeno-free,
functional hydrogel |
|
Use |
Organoid
culture |
|
Operation |
Ready-to-use
at room temperature |
|
Biocompatibility |
Biocompatible,
safe for animal studies |
|
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
|
Cell
Harvesting |
Easy cell recovery using VitroGel® Cell Recovery Solution |
|
pH |
Neutral |
|
Storage |
Store
at 2-8°C. Ships at ambient temperature |
|
Sizes |
Single
Vial: 10 mL |
|
Discovery
Kit: (2 mL) each of ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4 |
|
|
Number
of Uses |
(10
mL) 300 uses at 50 µL per well |
|
(2
mL) 60 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
ION Vital - Viability Kit
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K+) measurements. Ex/Em: 340/505 nm can be used to measure K+-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K+-free PBFI. It is 1.5X more selective for K+ over Naand works well in cytosolic conditions where [K+]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K+ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
AAK Supplement
Hydrogel supplements for use with the VitroGel® Angiogenesis Assay Kits
- AAK Supplement 1 - Without VEGFs (Dry Ice Shipping Required)
- AAK Supplement 2 - With VEGFs (Dry Ice Shipping Required)
Specifications
|
Formulation |
AAK
Supplement 1: |
|
Without
VEGFs |
|
|
AAK
Supplement 2: |
|
|
With
VEGFs |
|
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
|
Storage |
Store
at -20°C |
|
Shipping |
Requires
dry ice shipment. |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® MSC
Mesenchymal Stem Cell 3D Culture and Scale-up
VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). This hydrogel system can be used to make hydrogel cell beads for MSC scale-up. Microcarriers are not required for MSC scale-up.
VitroGel® MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydrogel system is compatible with most MSC culture media and tissue culture vessels. By using the VitroGel® Cell Recovery Solution, cell harvesting after 3D culture is simple and efficient.
3D cell culture process in 20 min – “Just add cells”
VitroGel® MSC is ready-to-use. Just mix with your cells.
Specifications
|
Formulation |
Xeno-free, functional
hydrogel |
|
Use |
3D cell culture, 2D
hydrogel coating, hydrogel-cell bead formation |
|
Operation |
Ready-to-use at room
temperature |
|
Biocompatibility |
Biocompatible, safe for
animal studies |
|
Cell Harvesting |
Easy cell recovery
using VitroGel® Cell Recovery Solution |
|
pH |
Neutral |
|
Storage |
Store at 2-8°C. Ships at
ambient temperature |
|
Sizes |
10 mL and 2 mL |
|
Number of Uses |
(10 mL) 300 uses at 50 µL
per well |
|
(2 mL) 60 uses at 50 µL
per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
Sodium Phosphate Monobasic, Anhydrous
- Appearance (Color): White to Off-White
- Appearance (Form): Crystalline Powder
- Purity: 98.0 - 103.0%
- pH (5% Aqueous): 4.1 - 4.5
- Solubility: Clear and colorless
- Identity (FTIR): Conforms to Structure
- Loss on Drying (105°C/4h): ≤ 0.5%
- Insoluble Matter: ≤ 2.0%
- Lead: ≤ 5 ppm
- Arsenic: ≤ 3 ppm
- Fluoride: ≤ 50 ppm
- Phosphorous: 24.5 - 26.5%
- RNase: None Detected
- DNase: None Detected
*Custom sizes available upon request.
DOT Information: Non-regulated.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® MMP High Concentration
Matrix metalloproteinases (MMP) sensitive biodegradable
hydrogel – tunable, xeno-free hydrogel, high concentration (3 mL kit)
- Dilution Solution TYPE 1 contains sucrose for best osmolarity
- Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium
VitroGel® MMP High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with matrix metalloproteinase-sensitive peptides. The enzyme-sensitive hydrogel can be degraded by several MMPs (MMP1, MMP2, MMP3, MMP9, and MMP13) and support different biological activities such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, etc. VitroGel® MMP High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection,
VitroGel® makes it possible to bridge the in vitro and in vivo studies with the
same platform system.
|
Contents |
VitroGel® MMP
High Concentration, 3 mL |
|
VitroGel® Dilution
Solution, 50 mL |
|
|
Hydrogel
Formulation |
Xeno-free
tunable hydrogel modified with matrix metalloproteinases sensitive peptide. |
|
HIGH
CONCENTRATION |
|
|
Use |
MMP
sensitive biodegradable hydrogel. |
|
Mix
& Match |
Can
be blended with other versions of VitroGel to create a multi-functional
hydrogel |
|
Operation |
Room
temperature |
|
Hydrogel
Strength |
10 to
4,000 Pa of G’ depending on dilution ratio. |
|
Dilute
with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different
concentrations. |
|
|
pH |
Neutral |
|
Color |
Transparent |
|
Cell
Harvesting |
20
min cell recovery using VitroGel® Cell Recovery Solution |
|
Injectable |
Injectable
hydrogel |
|
Storage |
Store
at 2-8°C. Ships at ambient temperature |
|
Number
of Uses |
Dilution
ratio: |
|
1:2 =
225 uses at 50 µL per well |
|
|
1:3 =
300 uses at 50 µL per well |
|
|
1:5 =
450 uses at 50 µL per well |
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
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