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ION Biosciences Fluo-4 K+ Salt
 
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable.
 
Fluo-4 is the most popular green fluorescent, calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.

Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.


Sodium Phosphate Dibasic, Heptahydrate

  • Appearance: White to Off-White Powder
  • Purity: ≥ 99.0%
  • pH (5% Aqueous) : 8.7 - 9.3
  • Solubility: Clear and Colorless
  • Identity (FTIR): Conforms to Structure
  • Loss on Drying (105°C/4 h): 44 - 50%
  • Lead: ≤ 10 ppm
  • Iron: ≤ 10 ppm
  • Chloride: ≤ 10 ppm
  • Phosphate: 35.0% - 39.-%
  • Sulfate: ≤ 50 ppm
  • RNase: None Detected
  • DNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.   
 

Item#:
ASPHOSODDIHEP

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® ORGANOID
 

ready-to-use, xeno-free (animal origin-free) hydrogel system for organoid culture

  • Xeno-free hydrogel for organoid formation
  • Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
  • Scalable protocol for lab automation
  • Easy 20-minute cell harvesting (enzyme-free)
  • VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.

VitroGel® ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel® ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

The VitroGel® ORGANOID hydrogels can work in conjunction with VitroGel® STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel® STEM to VitroGel® ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B.  Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution.  VitroGel® ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.


Which organoid hydrogel to choose

We still strongly suggest starting with the VitroGel® ORGANOID Discovery Kit (Item # VHM04-K) to make a quick screening to find the hydrogel version that is optimal for the organoid conditions.

The kit includes all four types of organoid hydrogels:

VitroGel® ORGANOID-1, VitroGel® ORGANOID-2, VitroGel® ORGANOID-3, VitroGel® ORGANOID-4)

Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.

Because there are a wide range of organoid cell resources (from stem cells, patient-derived tissue, co-culture, to PDX), it is hard to tell which organoid hydrogel would best perform the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for the organoid conditions.

From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids.

 

Specifications

Formulation

Xeno-free, functional hydrogel

Use

Organoid culture

Operation

Ready-to-use at room temperature

Biocompatibility

Biocompatible, safe for animal studies

Injection

Injectable hydrogel for in vivo studies and lab automation

Cell Harvesting

Easy cell recovery using VitroGel® Cell Recovery Solution

pH

Neutral

Storage

Store at 2-8°C. Ships at ambient temperature

Sizes

Single Vial: 10 mL

Discovery Kit: (2 mL) each of ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4

Number of Uses

(10 mL) 300 uses at 50 µL per well

(2 mL) 60 uses at 50 µL per well


TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

  

ION Vital - Viability Kit

Two-color, fluorescence-based assay to discriminate between live and dead cells.
 
ION Vital – Viability kit can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria. When following our protocol, ION Vital—Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.
ION Biosciences PBFI K+ Salt
 
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K+) measurements. Ex/Em: 340/505 nm can be used to measure K+-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K+-free PBFI. It is 1.5X more selective for K+ over Naand works well in cytosolic conditions where [K+]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K+ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

PBFI K+ salt is a membrane impermeable form of PBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection, or other methods.
ION Biosciences IPG-1 TMA Salt+

Yellow-green fluorescent, potassium (K+) indicator, membrane
 
impermeable. Lower K+ affinity than IPG-2 and IPG-4.
 
ION Potassium Green – 1 (IPG-1) is the best in class yellow-green fluorescent, intracellular potassium (K+) indicator for measuring changes at most intracellular K+ concentrations. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Kd = 18 mM) or IPG-4 (Kd = 7 mM).
 
IPG-1 TMA+ salt is a membrane impermeable form of IPG-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

AAK Supplement
 
Hydrogel supplements for use with the VitroGel® Angiogenesis Assay Kits

  • AAK Supplement 1 - Without VEGFs (Dry Ice Shipping Required)
  • AAK Supplement 2 -  With VEGFs (Dry Ice Shipping Required)
For studying angiogenesis, the AAK Supplements help determine cell growth and attachments as well for tube formation.


Specifications 

Formulation

AAK Supplement 1:

Without VEGFs

AAK Supplement 2:

With VEGFs

Use

Angiogenesis Assay, tube formation, invasion, animal injection

Storage

Store at -20°C

Shipping

Requires dry ice shipment.

 


TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

  
Item#:
ASAAKSUPP

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® MSC
 
Mesenchymal Stem Cell 3D Culture and Scale-up

VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs).  This hydrogel system can be used to make hydrogel cell beads for MSC scale-up.  Microcarriers are not required for MSC scale-up.

VitroGel® MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydrogel system is compatible with most MSC culture media and tissue culture vessels. By using the VitroGel® Cell Recovery Solution, cell harvesting after 3D culture is simple and efficient.

3D cell culture process in 20 min – “Just add cells”

VitroGel® MSC is ready-to-use. Just mix with your cells.

 

Specifications

Formulation

Xeno-free, functional hydrogel

Use

3D cell culture, 2D hydrogel coating, hydrogel-cell bead formation

Operation

Ready-to-use at room temperature

Biocompatibility

Biocompatible, safe for animal studies

Cell Harvesting

Easy cell recovery using VitroGel® Cell Recovery Solution

pH

Neutral

Storage

Store at 2-8°C. Ships at ambient temperature

Sizes

10 mL and 2 mL

Number of Uses

(10 mL) 300 uses at 50 µL per well

(2 mL) 60 uses at 50 µL per well



TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

  

ION Biosciences Fluo-2 AM

Green fluorescent, calcium (Ca²+) indicator, membrane permeable. Higher Ca²+ affinity than Fluo-4.
 
Fluo-2 is a green fluorescent, intracellular calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.
Brilliant Sodium Assays
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).


Sodium Phosphate Monobasic, Anhydrous

  • Appearance (Color): White to Off-White
  • Appearance (Form): Crystalline Powder
  • Purity: 98.0 - 103.0%
  • pH (5% Aqueous): 4.1 - 4.5
  • Solubility: Clear and colorless
  • Identity (FTIR): Conforms to Structure
  • Loss on Drying (105°C/4h): 0.5%
  • Insoluble Matter: ≤ 2.0%
  • Lead: ≤ 5 ppm
  • Arsenic: ≤ 3 ppm
  • Fluoride: ≤ 50 ppm
  • Phosphorous: 24.5 - 26.5%
  • RNase: None Detected
  • DNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.
 

Item#:
ASPHOSODMONANHY

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® MMP High Concentration
 

Matrix metalloproteinases (MMP) sensitive biodegradable hydrogel – tunable, xeno-free hydrogel, high concentration (3 mL kit)

  • Dilution Solution TYPE 1 contains sucrose for best osmolarity
  • Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® MMP High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with matrix metalloproteinase-sensitive peptides. The enzyme-sensitive hydrogel can be degraded by several MMPs (MMP1, MMP2, MMP3, MMP9, and MMP13) and support different biological activities such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, etc.  VitroGel® MMP High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.


Specifications 

Contents

VitroGel® MMP High Concentration, 3 mL

VitroGel® Dilution Solution, 50 mL

Hydrogel Formulation

Xeno-free tunable hydrogel modified with matrix metalloproteinases sensitive peptide.

HIGH CONCENTRATION

Use

MMP sensitive biodegradable hydrogel.

Mix & Match

Can be blended with other versions of VitroGel to create a multi-functional hydrogel

Operation

Room temperature

Hydrogel Strength

10 to 4,000 Pa of G’ depending on dilution ratio.

Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.

pH

Neutral

Color

Transparent

Cell Harvesting

20 min cell recovery using VitroGel® Cell Recovery Solution

Injectable

Injectable hydrogel

Storage

Store at 2-8°C. Ships at ambient temperature

Number of Uses

Dilution ratio:

1:2 = 225 uses at 50 µL per well

1:3 = 300 uses at 50 µL per well

1:5 = 450 uses at 50 µL per well

 

 TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

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