PR1MA™ Expanded Surface Tissue Culture Treated Roller Bottles

Designed to maximize cell growth efficiency while saving valuable lab space.

Featuring a uniform hydrophilic surface, these ESRB bottles support consistent cell attachment and growth, making them ideal for a wide range of cell culture applications. Available in multiple sizes and cap configurations, including vented and non-vented options, these roller bottles provide flexibility for both open and closed systems.

• Increase productivity with larger surface areas
• Reduce handling time and repetitive tasks
• Optimize laboratory space with high-capacity bottle options
• Maintain consistent and reliable cell growth conditions

Main Attributes

• Expanded surface (ESRB) design maximizes cell culture area and efficiency
• Horizontal ribbing promotes more even cell exposure compared to vertical rib designs
• Tissue culture treated surface ensures a uniform hydrophilic surface for reliable cell attachment
• Large knurled caps for easy handling and secure grip
• Vented and non-vented cap options for application flexibility
• Medical-grade virgin polystyrene construction for consistent performance
• Easy-to-read black printed graduations for accurate measurement
• Sterile, individually bagged packaging for immediate use 

Available Configurations

• 1900 cm² Expanded Surface Roller Bottles
• Available with non-vented or vented caps
• Expanded surface design increases available growth area
• Vented cap option includes a 0.22 uM hydrophobic membrane for gas exchange in open systems
• 4250 cm² Expanded Surface Roller Bottles
• Designed for large-scale cell culture applications
• Provides the equivalent growth area of five standard 850 cm² bottles while using 40% less plastic
• Fits into roller apparatuses that accommodate two standard 850 cm² bottles

Same Reliable Product, Now with Updated Part Numbers

PR1MA™ Tissue Culture Treated Roller Bottles

Sterile, vented, and non-vented options.

PR1MA™ Tissue Culture Treated Roller Bottles are designed to deliver reliable and consistent performance for cell culture applications. With a uniform hydrophilic surface, these bottles promote optimal cell attachment and growth, making them ideal for a wide range of laboratory workflows. Available in 490 cm² and 850 cm² sizes with both vented and non-vented cap options, these roller bottles provide flexibility for closed and open system applications.

• Supports consistent and reproducible cell culture results
• Flexible options for different workflows and system requirements
• Easy handling and reliable performance in laboratory environments
• Multiple sizes to accommodate varying culture volumes 

Main Attributes

• Tissue culture treated surface provides a uniform hydrophilic environment for consistent cell attachment
• Large knurled caps ensure easy handling and secure grip
• Vented cap options designed for open system applications with 0.22 uM hydrophobic membrane
• Medical-grade virgin polystyrene construction ensures quality and consistency
• Easy-to-read black printed graduations for accurate measurement
• Sterile packaging in individual bags or bulk sterile bags for convenience and protection 

Available Configurations

• 490 cm² Roller Bottles
• Vented (PR-RBVC-490) and non-vented (PR-RBNVC-490) cap options
• Vented caps include a 0.22 uM hydrophobic membrane for gas exchange
• 850 cm² Roller Bottles
• Vented (PR-RBVC-850) and non-vented (PR-RBNVC-850) cap options
• Vented versions support open system culture with membrane filtration
• Available in bulk (PR-RBVC-850-40): 20 bottles per sterile vacuum bag with dual dust bag layers, 2 bags per case

PR1MA™ Non-Treated Roller Bottles

Non-treated roller bottles with sterile, non-vented cap.

PR1MA™ Non-Treated Roller Bottles are designed for reliable cell culture applications requiring non-treated surfaces. Manufactured from medical-grade virgin polystyrene, these roller bottles provide consistent performance and durability while maintaining sterility with individual packaging.

Main Attributes

• Non-treated surface ideal for suspension cultures or specialized cell applications
• Non-vented cap ensures a secure seal during use
• Large knurled cap provides easy handling and improved grip
• Medical-grade virgin polystyrene construction for consistency and quality
• Easy-to-read black printed graduations allow accurate volume measurement
• Individually bagged and sterile for immediate use in laboratory environments 

Cell Culture Guidelines

• Recommended seeding density: 1 x 10³ to 1 x 10⁵ cells/cm²
• Initial adhesion phase (first 24 to 48 hours):
• Rotation speed: 0.5 to 1.0 rpm
• For cells with lower adhesion:
• Rotation speed: 0.3 to 0.5 rpm

*Note: These parameters are general recommendations; optimize conditions based on specific cell lines.

Cell Harvesting Protocol

• Rinse the bottle twice with PBS
• Add a dissociation solution (e.g., trypsin), ensuring full coverage of the bottle interior and pleats
• Place the bottle in an incubator and rotate at 0.3 to 0.5 rpm for 5 to 10 minutes
• After cell detachment, add an equal volume of cell culture medium to neutralize trypsin
• Rotate the bottle to collect the cell suspension
• Remove cells by pouring or pipetting

Our high strength glass bottles are available in many size configurations to suit various hybridization applications efficiently. Manufactured of low beta emitting borosilicate, they reduce exposure to radioactivity. Exposure is further limited by the leak proof closure system.

Our high strength glass bottles are available in many size configurations to suit various hybridization applications efficiently. Manufactured of low beta emitting borosilicate, they reduce exposure to radioactivity. Exposure is further limited by the leak proof closure system. 

Blue Cap = Polyethylene With O-Ring Seal; Temp. Resistance: -40°C to +140°C
Red Cap = Polyethylene With Teflon Seal; Temp Resistance: -45°C to +200°C