PR1MA™ Non-Treated Roller Bottles

Non-treated roller bottles with sterile, non-vented cap.

PR1MA™ Non-Treated Roller Bottles are designed for reliable cell culture applications requiring non-treated surfaces. Manufactured from medical-grade virgin polystyrene, these roller bottles provide consistent performance and durability while maintaining sterility with individual packaging.

Main Attributes

• Non-treated surface ideal for suspension cultures or specialized cell applications
• Non-vented cap ensures a secure seal during use
• Large knurled cap provides easy handling and improved grip
• Medical-grade virgin polystyrene construction for consistency and quality
• Easy-to-read black printed graduations allow accurate volume measurement
• Individually bagged and sterile for immediate use in laboratory environments 

Cell Culture Guidelines

• Recommended seeding density: 1 x 10³ to 1 x 10⁵ cells/cm²
• Initial adhesion phase (first 24 to 48 hours):
• Rotation speed: 0.5 to 1.0 rpm
• For cells with lower adhesion:
• Rotation speed: 0.3 to 0.5 rpm

*Note: These parameters are general recommendations; optimize conditions based on specific cell lines.

Cell Harvesting Protocol

• Rinse the bottle twice with PBS
• Add a dissociation solution (e.g., trypsin), ensuring full coverage of the bottle interior and pleats
• Place the bottle in an incubator and rotate at 0.3 to 0.5 rpm for 5 to 10 minutes
• After cell detachment, add an equal volume of cell culture medium to neutralize trypsin
• Rotate the bottle to collect the cell suspension
• Remove cells by pouring or pipetting