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Fluorescents, Dyes, and Assays
Essential Tools for Visualization, Detection, and Analysis
- Fluorescents include fluorophore-based reagents used for visualization, labeling, and fluorescence detection in workflows such as flow cytometry
- Dyes are used for conjugation, visualization, and molecular labeling applications across immunofluorescence, IHC, and in vivo workflows
- Assays are made to support quantitative and qualitative analysis in life science and molecular biology applications
Why choose MIDSCI™ for fluorescents, dyes, and assays? MIDSCI™ offers a broad range of high-quality fluorescent reagents and assay products designed to support every lab.
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ION Biosciences IPG-1 TMA Salt+
Yellow-green fluorescent, potassium (K+) indicator, membrane
impermeable. Lower K+ affinity than IPG-2 and IPG-4.
ION Potassium Green – 1 (IPG-1) is the best in class yellow-green fluorescent, intracellular potassium (K+) indicator for measuring changes at most intracellular K+ concentrations. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Kd = 18 mM) or IPG-4 (Kd = 7 mM).
IPG-1 TMA+ salt is a membrane impermeable form of IPG-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASIPG1TMAST
ION Biosciences Fluo-2 AM
Green fluorescent, calcium (Ca²+) indicator, membrane permeable. Higher Ca²+ affinity than Fluo-4.
Fluo-2 is a green fluorescent, intracellular calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.
Brilliant Sodium Assays
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Item#:
ASBRTHAS
ION Biosciences PBFI AM
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K) measurements. Ex/Em: 340/505 nm can be used to measure K-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K-free PBFI. It is 1.5X more selective for K over Na, and works well in cytosolic conditions where [K]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K) measurements. Ex/Em: 340/505 nm can be used to measure K-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K-free PBFI. It is 1.5X more selective for K over Na, and works well in cytosolic conditions where [K]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Item#:
ASPBFIAM
ION Biosciences ING-2 AM
Yellow-green fluorescent, sodium (Na+) indicator, membrane permeable. Higher Na+ affinity than ING-1.
ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na+) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na+ indicator for high-throughput screening applications targeting Na+ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.
Item#:
ASIGN2AM
Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.
- Choose britelite plus:
- Half-life = 30 min
- Brightest signal = when looking for maximum sensitivity
- Shortest half-life = for continuous processing of samples
- Choose neolite:
- Half-life = 150 min
- High signal intensity, longer lasting signal = good balance
- For both continuous and batch processing of samples
- Best robustness (less dependent on mixing quality)
- Less sensitive to phenol red
- Can be stored 1 month at 22°C
- Choose steadylite plus:
- Half-life = 240 min
- Longest signal half-life = for batch processing of samples
- Super-sensitivity not needed
Item#:
ASLUMASSAY1
Perkin Elmer's ATPlite simple and sensitive system is an excellent kit to determine cell number due to proliferation or toxicity. It effectively replaces the MTT assay and can be used instead of the 3H thymidine assays with additional analysis.
- Choose ATPlite (2 steps):
- Half-life> 4 hours
- Longest half - life = for batch processing of samples
- 2 step system with separate lysis and detection steps
- More suited for Academia Choose
- ATPlite 1step:
- Measure within 30 min
- Brightest signal (~3x the counts of ATPlite 2Steps) = when looking for maximum sensitivity
- For continuous processing of samples
- True 1 step system = More suited to HTS
6016736 |
6016731 |
6016739 |
6016943 |
6016941 |
6016947 |
6016949 |
|
| Assay Protocol | 1-step |
1-step |
1-step |
2-step |
2-step |
2-step |
2-step |
| Automation Compatible | Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
| Detection Method | Luminescence |
Luminescence |
Luminescence |
Luminescence |
Luminescence |
Luminescence |
Luminescence |
| Experimental Type | In vitro |
In vitro |
In vitro |
In vitro |
In vitro |
In vitro |
In vitro |
| One Unit Contains | 10 mL |
100 mL |
1000 mL |
300 assays |
1000 assays |
5000 assays |
10000 assays |
| Product Brand Name | ATPlite |
ATPlite |
ATPlite |
ATPlite |
ATPlite |
ATPlite |
ATPlite |
| Shipping Condition | Ambient |
Ambient |
Ambient |
Ambient |
Ambient |
Ambient |
Ambient |
| Unit Size | 100 (96-well) / 400 (384-well) |
1,000 (96-well) / 4,000 (384-well) |
10,000 (96-well) / 40,000 (384-well) |
300 (96-well) / 1200 (384-well) |
1,000 assay points |
5,000 (96-well) / 20,000 (384-well) |
10,000 (96-well) / 40,000 (384-well) |
| Vol Capacity | 10 mL |
100 mL |
1000 mL |
N/A |
N/A |
N/A |
N/A |
Item#:
ASLUMASSAY3
Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.
- Choose britelite plus:
- Half-life = 30 min
- Brightest signal = when looking for maximum sensitivity
- Shortest half-life = for continuous processing of samples
- Choose neolite:
- Half-life = 150 min
- High signal intensity, longer lasting signal = good balance
- For both continuous and batch processing of samples
- Best robustness (less dependent on mixing quality)
- Less sensitive to phenol red
- Can be stored 1 month at 22°C
- Choose steadylite plus:
- Half-life = 240 min
- Longest signal half-life = for batch processing of samples
- Super-sensitivity not needed
Item#:
ASLUMASSAY2
ION Biosciences Fura-2 LR AM
Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane permeable.
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
ION Biosciences ICR-1 AM
Red fluorescent, calcium (Ca²+) indicator, membrane permeable. Optimal for multiplexing and tissue imaging. Fluorescence lifetime and multiphoton compatible.
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²+) indicator for intracellular Ca²+ measurements.
ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
Item#:
ASICR1AM
ION Biosciences Fluo-4 AM
Green fluorescent, calcium (Ca²+) indicator, membrane permeable.
Fluo-4 is the most popular green fluorescent, intracellular calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.
Item#:
ASFLUO4AM
Calcein AM
Green fluorescent, live cell indicator.
Calcein AM is a membrane-permeant, non-fluorescent form of calcein that is converted to green fluorescent calcein in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein is well retained within the cytosol of most healthy cells with intact cell membranes.
Item#:
ASCALCEINTR
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