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Fluorescents, Dyes, and Assays

Essential Tools for Visualization, Detection, and Analysis

  • Fluorescents include fluorophore-based reagents used for visualization, labeling, and fluorescence detection in workflows such as flow cytometry
  • Dyes are used for conjugation, visualization, and molecular labeling applications across immunofluorescence, IHC, and in vivo workflows
  • Assays are made to support quantitative and qualitative analysis in life science and molecular biology applications

Why choose MIDSCI™ for fluorescents, dyes, and assays? MIDSCI™ offers a broad range of high-quality fluorescent reagents and assay products designed to support every lab.

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ION Biosciences IPG-2 TMA+ Salt
 
Yellow-green fluorescent, potassium (K+) indicator, membrane
 
impermeable. Lower K+ affinity than IPG-4 and higher affinity than IPG-1
 
ION Potassium Green – 2 (IPG-2) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Kd = 50 mM) and lower affinity than IPG-4 (Kd = 7 mM).
 
IPG-2 TMA+ salt is a membrane impermeable form of IPG-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods
Item#:
ASIPG2TMAST
 
IPG-2 TMA+ Salt Safety Data Sheet
IPG-2 TMA+ Salt Product Specifications
SBFI AM
 
Ratiometric sodium (Na+) indicator, membrane permeable
 
SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Item#:
ASSBFIAM
 
SBFI AM PROTOCOL
SBFI AM Safety Data Sheet
SBFI AM Product Specifications

ION Ethidium Homodimer I

Red fluorescent, dead cell stain.
 
Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that selectively labels dead cells with compromised plasma membranes.
Item#:
ASIONETHIDI
 
ETHIDIUM HOMODIMER I Safety Data Sheet
ETHIDIUM HOMODIMER I Product Specifications
ION Biosciences IPG-4 TMA+ Salt

Yellow-green fluorescent, potassium (K+) indicator, membrane
 
impermeable. Higher K+ affinity than IPG-1 and IPG-2.
 
ION Potassium Green – 4 (IPG-4) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-4 has the highest affinity (Kd = 7 mM) among IPG analogues – IPG-2 (Kd = 18 mM) and IPG-1 (Kd = 50 mM).
 
IPG-4 TMA+ salt is a membrane impermeable form of IPG-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods. IPG-4 has been successfully used as a sensor for extracellular K+.
Item#:
ASIPG4TMAST
 
IPG-4 TMA+ Salt Safety Data Sheet
IPG-4 TMA+ Salt Product Specifications

Western Lightning Plus (NEL103):
·  Twice the sensitivity of standard products 

·  Direct protocol transfer 

·  ~ 1 picogram sensitivity

·  > 2 hr signal duration for re-exposing film

·  Working solution stability 8 hr at room temperature 

·  PVDF or Nitrocellulose membranes 

·  Non-toxic (no msds required)

 

Western Lightning Pro (NEL120)
·  Mid-range sensitivity, with broad dynamic range

·  Detection of less than 1 picogram amount of protein

·  Long, stable signal > 12 hr for re-exposing film 

·  Stable at room temperature >1 year 

·  Working solution stable for > 24 hr at room temperature

·  Non-toxic (no msds required) 

·  PVDF or Nitrocellulose membran
Item#:
ASWESTERNKITE
Thallos AM
 
Best in class, green fluorescent, thallium (Tl+) indicator, membrane permeable. Works with potassium (K+), sodium (Na+), and monovalent cation channels, and transporters.
 
Thallos is a green fluorescent, intracellular thallium (Tl+) indicator, and has been the gold standard for fluorescence-based potassium (K+) channel HTS for nearly 2 decades. Thallos also delivers outstanding results for a wide variety of monovalent cation (sodium – Na+) channels, transporters, and GPCRs.
Item#:
ASTHALLOSAM
 
THALLOS AM PROTOCOL
Thallos AM Safety Data Sheet
Thallos AM Product Specifications

ION Biosciences Fluo-2 K+ Salt

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable. Higher Ca²+ affinity than Fluo-4.

Fluo-2 is a green fluorescent, calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.

Fluo-2 K+ salt is a membrane impermeable form of Fluo-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Item#:
ASFLUO2AMST
 
Fluo-2 K+ Salt Safety Data Sheet
Fluo-2 K+ Salt Product Specifications
ION Biosciences Fura-2 LR K+ Salt
 
Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane impermeable.
 
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
 
Fura-2 LR K+ salt is a membrane impermeable form of Fura-2 LR that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFURA2LRKST
 
LR K+ Salt Safety Data Sheet
LR K+ Salt Product Specifications
ION Biosciences BCECF AM

BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings.
Item#:
ASBCECFAMPN
 
BCECF AM Safety Data Sheet
BCECF AM Product Specifications
ION Biosciences Brilliant Sodium Flex Kit
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Item#:
ASBRSOFXK
 
BRILLIANT THALLIUM FLEX_PROTOCOL
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