Hoefer® Standard Transfer Tank with Cooling Chamber

TE62 Standard Transfer Tank with Cooling Chamber quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes while providing excellent temperature control.

The TE62 is designed to accept up to four cassettes simultaneously with cooling. Each cassette holds a 15 x 21 cm gel, or as many as four 7 x 10 cm mini gels.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes - ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger - provides excellent temperature control when used with an external cooling water bath  

Hoefer® Standard Transfer Tank with Cooling Chamber Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk 

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Azure Aqua Transfer Cell

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

Includes:

• 2 transfer cassettes
• 6 sponges
• 2 Azure Aqua cooling units
• Tank and upper lid with power cables
• Transfer Electrode Cores

Features

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

Chemical reagent compatibility

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

Specifications
 

• 302 nm midrange UV wavelength (optimal for EtBr excitation)
• Dual intensity settings (50% or 100%)
• Hinged UV Safety cover
• Use with SmartDocTM enclosure (E5000-SD) for gel imaging with a smart phone
• Compatible DNA stains: EtBr, SmartGlow™ LD and PS stain, Gel Red, Gel Green, SYBR® Green, SYBR® safe, and Diamond™ Nucleic acid Dye
• 2-yr warranty

This new UV transilluminator features a space-saving design while having 16 x 19 cm UV viewing surface to accommodate large gels or multiple smaller gels. The illuminated surface is comprised of a special black glass which is easy to clean, durable and scratch resistant.

Furthermore, an array of high intensity UV bulbs coupled with an optimized filter provides even illumination across the viewing surface and allows detection of small sample quantities. The dual intensity switch can be used to lower the UV intensity to 50% to reduce DNA damage during long exposure times, or switch to high intensity (100%) for viewing weak fluorescent signals or for photo documentation.

The acrylic UV blocking cover allows safe viewing of samples and has two friction hinges on the front edge to allow angling of the cover for gel access. The cover can be positioned to 90° (straight up) to fit a gel imaging hood, such as the SmartDoc™ imaging enclosure (E5000-SD).

Specifications
 

Light Source	UV
Wavelength	302 nm
Viewing Surface	16 x 19 cm
Ext. Dimensions	10.5 x 13 x 5.5 in | 26.5 x 32.5 x 14 cm
Electrical	120 or 230V, 50-60Hz
Warranty	2 years

 

Azure Aqua Quad Mini-Cell

Vertical Gel Running System

Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.

Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Includes:

• 4 gel casting stands and casting frames buffer dam
• 5 spacer plates (1mm)
• 10 short glass plates
• 5 15-well combs
• 5 10-well combs
• 5 sealing gaskets
• 5 gel shovels
• Tank and lid with power cables
• Primary and Secondary Electrode Cores

Features

• The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
• The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
• Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Specifications
 

Parameters	Azure Aqua Quad Mini-Cell Specs
Unit dimensions (W x L x H)	13.5 cm x 17.5 cm x 19 cm
Weight	2.2 lbs
Recommended power supply	Azure Aqua Power Supply 600V
Number of gels	1-4 precast (most major brands) or handcast gels
Tank buffer volume	2 gels: 540 mL | 4 gels: 1100 mL
Maximum gel size	6.7 cm x 8.6 cm
Typical run times	30-45 minutes at 200V
Gel cassette size (WxL)	10 cm x 8 cm

 

Hoefer® Semi-Dry Transfer Units

Hoefer® Semi-Dry Transfer Units are efficiently designed to use minimal buffer to transfer proteins from polyacrylamide gels onto membranes (either nitrocellulose or PVDF) in less than an hour. They support as many as two layers of gels being transferred simultaneously. The TE70XP has a 14 x 16 cm maximum transfer area, which can handle up to four mini gels using the stacked format. The TE77X and TE77XP have a 21 x 26 cm transfer area for a capacity of up to 12 mini gels at one time. 

• Included safety circuit breaker—limits voltage and current from the user's power supply, preventing electrical damage to the transfer unit
• Requires minimal current—does not generate the excessive heat that can dry out the transfer stack and halt transfer or damage transfer units 
• Durable iridium oxide and stainless steel electrodes—allow for contamination free, consistent transfer
• Vented electrodes—prevent buildup of bubbles which may impair transfer
• Minimal buffer requirements—reagent cost and preparation time are reduced
• The TE70XP and TE77XP Semi-Dry Transfer Units have an intelligent built-in power supply that prevents the stack from overheating by monitoring the transfer status 

All units include

• Molded Base with Platinum-Coated Titanium Anode and High Voltage Leads
• Hinged Lid with Stainless Steel Cathode  

TE77X includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk
• Hinged Lid with Stainless Steel Cathode, 1/ea

TE70XP includes

• Blotting Paper (14 x 16 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (16.5 x 18.5 cm), 2/pk 

TE77XP includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk

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Azure Aqua Power Supply

Designed for powering electrophoresis and Western blotting transfer modules

This power supply can create and save protocols for all your gel and blotting experiments. Designed with you in mind, features a pause/resume function for flexibility.

Power supply includes output range of 5–600V, 1–1200mA, 1–500W, LCD readable voltage, current, power, and four power connections to run multiple experiments at once. Able to control constant power, current, or voltage.

Features

The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and transfer modules. The power supply can create and save protocols for all your gel and blotting experiments and includes a pause/resume function for flexibility.

Azure Power Supply includes an output range of 5-600V, 1-1200mA, 1-500W, LCD readable voltage, current, power, and 4 connections for electrophoresis and transfer.

Specifications
 

Accuris SmartBlue™ Transilluminators

Accuris SmartBlue™ Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes.  These new dyes, such as SmartGlow™, SYBR Green, Gel Green, have an excitation wavelength of 465-470 nm (visible blue light), and are safer to use than Ethidium Bromide.

• Blue light, safe for nucleic acids
• Uniform illumination (UI Technology)
• Optimal wavelength (465nm) for common safe stains
• Adjustable position, amber filter covers
• Optional amber viewing glasses: E4000-VG1

Safer for samples, safer for you: Accuris SmartBlue™ Transilluminators are the perfect partner for the most common safe DNA stains. These instruments emit blue light at a wavelength of 465 nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  SmartBlue™ transilluminators can also work for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images.   Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*

Adjustable filter cover: Both the E4101 and SB4500 transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers can be set to a flat position for general gel viewing or can be set at an angle to provide easy access to the gel for band excision.  The SB4500 has a two-position filter cover, and the E4101 features a hinged filter cover.

Optional amber viewing glasses: Our E4000-VG1 SmartBlue™ Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place.  These convenient glasses are recommended for users that are frequently excising DNA bands from gels.  SmartBlue™ Plus glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.

UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.

Reliable, durable, and long-lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue™ systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Transilluminators are covered by a 2 year warranty.

* SmartBlue™ products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 

Specifications 

Light Source	High Intensity Blue LEDs
Emission Filter Cover	Amber
Wavelength	Peak at 465 nm
E4000 Viewing Surface	17 x 12 cm
E4000 Ext. Dimensions	30.5 x 21.5 x 5 cm
E4101 Viewing Surface	10.5 x 10.5 cm
E4101 Ext. Dimensions	17 x 12 x 5 cm
Electrical	100 to  240V, 50-60Hz
E4000 Weight	1.2kg / 2.6lbs
E4101 Weight	0.34kg / 0.75lbs
Warranty	2 years

 
 Item # SB4500 is proudly made in the USA! Click here for more made in America products!
 

Hoefer® Gradient Makers

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride.

• Made of heavy acrylic plastic with high-quality, leak-free Teflon® valves
• Flat base provides stability on the magnetic stir. Comes with a support rod for attaching to a ring stand
• Takes Luer or 4 mm ID tubing. With included adapters, it can also take 2 mm ID or 22-gauge tubing

SG15, SG30, SG50 and SG100 include

• Gradient Maker with Teflon® Valves, 1/ea
• Adapter Barbed Fitting, 1/ea
• Luer Outlet Fitting, 1/ea
• 22-gauge Needle, 1/ea
• Support Rod, 1/ea 

The 500 mL SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base.

• An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers
• Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns
• Ideal for use with the SE615 Multiple Gel Caster  

SG500 includes

• Gradient Maker with Push-Pull Valve, 1/ea
• Hose Clamps, 4/pk

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Hoefer® Gel Casters

Enable several gradient or homogeneous gels to be cast simultaneously. Choice of multiple-gel casters or dual-gel casters.

Gel Casters for Small Format Vertical Electrophoresis Systems (SE250, SE260, SE300)

Multiple-Gel Casters

• Pour uniform gels from the top; pump gradient gels into an inlet port at the bottom of the unit
• Sandwiches fill at the same time and at the same rate from the same solution
• The result: identical gels
• Glass plate sandwiches seal leak-free without the use of messy grease or tape
• Once cast, gels can be wrapped in plastic and refrigerated for several weeks

Dual-Gel Caster (SE245)

• Cast one or two gels
• Accommodates both 10 x 8 cm and 10 x 10.5 cm plates

*For use with the SE250 and SE260 Series only.

Gel Casters for SE600 and SE400 Series Vertical Units (SE600, SE400)   

Multiple-Gel Casters

• SE675 casts up to four 16 cm long gels
• SE615 casts up to ten 16 cm long gels
• Easy to use and leak-free
• Cast homogeneous or gradient gels. Pour homogeneous gels directly into the top of the casting chamber: pump gradient gels through an inlet port at the bottom

Dual-Gel Caster (SE6015)

SE6015 Dual Gel Caster is included when you order an SE600 series unit. It can also be ordered separately.

• Casts two 8, 16, or 24 cm long gels at a time
• Double the capacity with optional divider plates
• Easy to use and leak-free
• Glass Sandwichers seal into the casting 

*For use with the SE600 Series only.

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Hoefer® Combs for SUB15 and SUBHT Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

MC = Microtiter spacing for use with a multichannel pipette
Sample volume listed is for a 5 mm deep well

For use with SUB15 and SUBHT Submarine Gel Electrophoresis Systems

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Hoefer® Combs for SUB20 and SUB20C Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB20 and SUB20C Submarine Gel Electrophoresis Systems

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Hoefer® Combs for SUB10 Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB10 Submarine Gel Electrophoresis Systems

Proudly made in the USA! Click here for more made in America products!