The Genomic DNA Kit for Blood or Cultured Cells provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 µ g from 200 µ l of whole human blood and up to 50µ g of DNA from 200µ l of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.

MagBio HighPrep™ Viral/Bacterial DNA/RNA Kit

, Beads

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VitroGel® Dilution Solution (50 mL)
 
For hydrogel concentration adjustment with the high concentration hydrogels.

• Type 1 - Contains sucrose to maintain the best osmolarity.
• Type 2 - Sucrose-free formulation for cells sensitive to the sugar level in the medium.

VitroGel® Dilution Solution is a ready-to-use solution to be used with the VitroGel® High Concentration hydrogels to adjust the hydrogel concentration for different hydrogel strength.

The solution is room temperature stable with a neutral pH, which maintains a good physiological condition for hydrogel formation and can accelerate the hydrogel formation in combination with the cell medium. The Dilution Solution can be used to prepare a thin gel coating plate or injectable hydrogel with the high concentrations hydrogels.

Specifications

• Ready-to-use to adjust the concentration of VitroGel® High Concentration hydrogels
• Neutral pH
• Room temperature stable
• Transparent
• Compatible with VitroGel® system for 2D & 3D cell culture and hydrogel injection
• Ships and store at room temperature
• Size: 50 mL

PR1MA™ Calcium Chloride Anhydrous

CAS Number: 10043-52-4
Molecular Weight: 110.98
Chemical Formula: CaCl₂
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay (Titration): ≥ 96.0 %
• Titratable Base: ≤ 0.006 meq/g

PR1MA™ Bromophenol Blue [3',3",5',5"-Tetrabromo-phenolsulfonphthalein]

Common Name: 3',3",5',5"-Tetrabromo-phenolsulfonphthalein
CAS Number: 115-39-9
Molecular Weight: 669.96
Chemical Formula: C₁₉H₁₀Br₄O₅S
Solubility: Methanol, ethanol
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Pink to Red Crystalline Powder
• Clarity of Solution: Pass
• Visual Transition Interval (pH 3.0 - 4.6): Pass

PR1MA™  Paromomycin Sulfate

CAS Number: 1263-89-4
Molecular Weight: 615.6
Chemical Formula: C₂₃H₄₅N₅O₁₄ • H₂SO₄
Solubility: Water
Storage Temp: Room Temperature

Aminoglycoside antibiotic with mode of action similar to Kanamycin and Neomycin. Used as a selective agent for the incorporation of the NPT II (APH3') gene in plant tissue. Plant culture tested.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Powder
• Assay: ≥ 675 U/mg
• Identification: Conforms
• Loss on Drying: ≤ 5.0 %
• pH 3.0% Solution @25^oC: 5.0 -7.5
• Specific Optical Rotation: +50.0 - +55.0°

PR1MA™  Bacitracin

CAS Number: 1405-87-4
Molecular Weight: 1422.69
Chemical Formula: C₆₆H₁₀₃N₁₇O₁₆ S
Solubility: Water, Ethanol, and Methanol
Storage Temp: 2-8°C

Interferes with bacterial cell wall synthesis. Active against gram positive bacteria. Toxic to plant cells.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to pale buff powder
• Identification: Pass test
• pH: 5.5 - 7.5
• Loss on Drying: ≤ 5.0 %
• Residue on Ignition: ≤ 0.5 %
• Bacitracin A: ≥ 40.0 %
• SUM of bacitracin A, B1, B2 and B3: ≥ 70.0 %
• Limit of early eluting peptides: ≤ 20.0 %
• Limit of Bacitracin F: ≤ 6.0 %
• Microbial Assay (Dried Basis): ≥ 65.0 IU / mg
• Heavy Metals : ≤ 30 ppm
• Total Viable Aerobic Count: ≤ 100 CFU/g
• Particle Size (< 20 µm by volume): ≥ 99.0 %
• Crystallinity : Pass
• Appearance of Solution: Clear, colorless to slight hazy yellow
• Source: Bacillus Licheniformis
• Non Animal Origin: Non Animal Origin
• Grade: USP
• Country of Origin : Report

PR1MA™  Polymixin B Sulfate

CAS Number: 1405-20-5
Molecular Weight: 1385.61
Chemical Formula: C₅₅H₉₆N₁₆O₁₃ • 2H₂SO₄
Solubility: Water
Storage Temp: 2-8°C

Mixture of sulfates of polypeptides from certain strains of Bacillus polymixa. Active against gram negative bacteria.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Light Yellow Powder
• Identification: Pass Test
• pH (0.5% Solution): 5.0 - 7.5
• Loss on Drying: ≤ 7.0 %
• Single Impurity: ≤ 3.0 %
• Total impurities: ≤ 17.0 %
• Bacteria Colonies: ≤ 10/g
• Ecoli Colonies: Should not be found
• Fungi and Yeast colonies: ≤ 10/g
• Assay, HPLC (on dried basis) (Sum of polymxin B1,B2, B3 and B1-1): ≥ 80.0 %
• Assay, HPLC (on dried basis) (Polymyxin B3): ≤ 6.0 %
• Assay, HPLC (on dried basis) (Polymyxin B1-1): ≤ 15.0 %
• Assay (Dried Basis): ≥ 6000 U/mg
• Assay (Wet Basis): Report U/mg
• Non Animal Origin: Non Animal Origin
• Grade: USP
• Country of Origin: Report

PR1MA™ DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C₄H₁₀O₂S₂
Solubility: Water
Storage Temp: 2-8°C

Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane, protecting protein sulfhydryls, thereby restoring enzyme activity lost by oxidation of the groups.

Research or further manufacturing use only, not for food or drug use.

• Appearance: A white free flowing powder, hygroscopic
• Solubility: Freely soluble in methanol, methylene chloride
• Clarity in water: 5% (w/v) solution in water is clear and colorless
• Identification by TLC: Rf value of the sample is comparable with that of the standard
• Melting Range: 37.0 - 44.0 °C
• Related Substance (UV Spectroscopy- Content of Oxidised DTT): 0.4 %
• Assay (By Iodimetry): ≥ 98.0 %
• Country of Origin: Report

PR1MA™ Puromycin Dihydrochloride

CAS Number: 58-58-2
Molecular Weight: 544.43
Chemical Formula: C₂₂H₂₉N₇O₅ • 2HCl
Solubility: Water
Storage Temp: -20°C

Amino nucleoside antibiotic allows the selection of cells expressing the pac gene. Inhibits the growth of gram-positive bacteria and various animal and insect cells.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Powder
• Identification (TLC): Conforms
• Melting Point: 168 - 182 ^oC
• Purity (HPLC): ≥ 98.0 %
• Solubility: Clear, colorless or light yellow solution
• Water (KF): ≤ 12.0 %

PR1MA™  Blasticidin S Hydrochloride Powder

CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C₁₇H₂₆N₈O₅ • HCl
Solubility: Water
Storage Temp: -20°C

• Appearance: White Powder
• Endotoxin : ≤ 1.0 EU / mg
• Identification: Pass Test
• Potency: ≥ 850 ug / mg
• Purity (HPLC): ≥ 98.0 %
• Solubility: Clear, Slightly yellow solution at 5 mg / mL in water

PR1MA™ Ammonium Acetate

CAS Number: 631-61-8
Molecular Weight: 77.08
Chemical Formula: C₂H₇NO₂
Solubility: Water
Storage Temp: 2-8°C

Widely used reagent in molecular biology and chromatography.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline hygroscopic powder
• Assay: ≥ 97.0 %
• Chloride (Cl): ≤ 0.0005 %
• Heavy Metals (as Pb): ≤ 0.0005 %
• Insoluble Matter: ≤ 0.005 %
• Iron (Fe): ≤ 0.0005 %
• Nitrate: ≤ 0.001 %
• pH, 5% solution in H₂O @25 ºC: 6.7 - 7.3
• Residue after Ignition: ≤ 0.01 %
• Sulfate: ≤ 0.001 %