MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
• Tube volume: 3ml
• Dialysis volume: 0.1-3ml
• Min. sample size for extraction: 20µg
• Max. gel slice: 2cm x 1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

Bullseye PREMIUM HS-Taq DNA Polymerase Master Mix with HS Buffer I

Store at -20°C. For in-vitro laboratory use only

Bullseye HS Taq Polymerase, 2X Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye HS Taq Polymerase Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer I. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.

Bullseye HS Taq Polymerase Mix is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Bullseye" HS Taq Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of HS Taq Pol, 2x Mix

Tris-HCl pH 8.5, (NH4)2S04, 3.0mM MgCl2, 0.2% Tween 20Ã, 0.4 mM dNTPs, 0.2 units/uL HS Hot Start DNA Polymerase Stabilizer

Suggested Protocol using HS Taq Pol, 2x Mix

This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.

• Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
• The table below shows the reaction set up for a final volume of 50 mL.
• Important: Mix the solutions completely before use to avoid localized concentrations of salts.

  1. Set up each reaction as follows:

The Total RNA MINI and MAXI kits (Plant) provide a simple and fast method to isolate total RNA from plant tissue and cells. Samples are ground in liquid nitrogen and filtered to remove debris. In the presence of a binding buffer and chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix of the spin column. The optional DNase treatments can remove DNA residues and the contaminants can be washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation, and the entire procedure can be completed within 60 minutes. The purified total RNA is ready for RT, RT-PCR, Real Time PCR and northern blotting.

• Sample: Gram (+) positive and Gram (-) negative bacterial cells
• Yield: Up to 60 µg of RNA - (1 x 10 Escherichia coli: 40-45 µg, 1 x 10 Bacillus subtilis: 50-55 µg)
• Convenient: Includes Lysozyme and Bacteria Lysis Buffer
• Format: RNA spin columns
• Operation Time: Within 30 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months, Lysozyme should be stored at -20°C for extended periods

The rBAC Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative and Gram (+) positive bacteria. The provided Lysozyme and Bacteria Lysis Buffer will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Detergents and chaotropic salt are used to further lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and is ready for use in a variety of subsequent reactions.

Quality Control
The quality of the rBAC Mini RNA Bacteria Kit is tested on a lot-to-lot basis by isolating RNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

*Lysozyme should be stored at -20°C for extended periods. Add Lysozyme to Bacteria Lysis Buffer immediately prior to use. Once Lysozyme is mixed with Bacteria Lysis Buffer, the solution can be stored for 2 weeks at 4°C.

**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

• Sample: Variety of yeast and other fungus species
• Yield: Up to 30 µg of RNA (5 x 107 Saccharomyces cerevisiae: 20 µg)
• Convenient: Includes Sorbitol Buffer to reduce sample preparation time
• Format: RNA spin columns
• Operation Time: Within 70 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months

The rYeast Total RNA Mini Kit was designed for total RNA purification from yeast and a wide variety of other fungus species. Sorbitol Buffer is included with the kit to reduce sample preparation time and minimize hands on time. Detergents and chaotropic salt are used to lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. The entire procedure can be completed within 70 minutes and the purified RNA is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis.

Quality Control
The quality of the rYeast Total RNA Mini Kit is tested on a lot-to-lot basis by isolating RNA from Saccharomyces cerevisiae (5 x 10  ) harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µL aliquot of purified RNA from a 50 µL eluate is analyzed by electrophoresis on a 0.8% agarose gel.

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

IBI's Viral Nucleic Acid Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids and the supernatant of viral-infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays.

IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in IBI Isolate without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Quality Control
IBI Tri-Isolate is tested on a lot-to-lot basis. An Escherichia coli (1 x 109) culture (OD600=1.3, 1 mL) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by IBI Isolate homogenization. RNA is then purified using a spin column procedure. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages

• Purify total RNA within 15 minutes without chloroform phase separation or isopropanol RNA precipitation
• Up to: 200 µL (blood, buffy coat, serum, plasma), 5 x 106 (cultured cells), 10-50 mg (tissue), 1 x 109 (bacteria cells)
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays,
• Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Components and Storage

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer prior to initial use.

• Excellent all-purpose amplification enzyme
• Thermostable recombinant DNA polymerase from Thermus aquaticus
• Exhibits very high activity in primer extension
• Has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity
• Does not have 3' to 5' exonuclease activity-no proofreading ability
• Leaves an A-overhang, which makes the enzyme ideal for TA cloning
• Includes MgCl² in buffer

This brand is being discontinued, please contact us for other options.

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!

Bullseye PREMIUM Taq DNA Polymerases

Bullseye Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

Bullseye Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A¢ overhang, which makes the enzyme ideal for TA cloning.

The 10X Reaction Buffer provided does not contain Mg+2. 25 mM MgCl2 is supplied separately.

10X Mg++ Free Standard Buffer

100 mM Tris-HCl pH 8.3, 500 mM KCl.

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Storage and Dilution Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5%NP40, 50% glycerol.

Quality Control

Each lot of Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.

Suggested Protocol using Taq DNA Polymerase

This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.

Table 1. Reaction components (master mix & template DNA)

Table 2. MgCl2 concentration in a 50 mL reaction

Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a certain MgCl2 concentration is required.

2x Master Mix Kit (1.5 mM MgCl2) 

Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)

Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of 2x Taq Master Mix

150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2% Tween 20Ò

4 mM dNTPs

2 units/µL AS ONE Taq polymerase

Inert Red Dye & Stabilizer (BE180303 only)

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.