• Sample Size: 250-500 mg of soil
• Expectant Yield: up to 5 µg of genomic DNA
• Format: beadbeating tubes, PCR inhibitor removal columns and genomic DNA spin columns
• Operation Time: within 40 minutes
• Elution Volume: 30-100 µl
• Kit Storage: dry at room temperature (15-25°C) for up to 18 months without showing any reduction in performance

The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized and disrupted using SL1 lysis Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal Buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

Introduction
IBI Plant Isolate provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP. Phenol extraction is not required and the entire procedure can be completed within 50 minutes.

Quality Control
IBI Plant Isolate is tested on a lot-to-lot. 50 mg of fresh Arabidopsis leaves are initially ground in IBI Plant Isolate. A 15 µL aliquot of extracted genomic DNA from a µL eluate is analyzed by electrophoresis on a 1% agarose gel.

Advantages

• High molecular weight genomic DNA extraction from a variety of plant species 
  
• Sample: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  
• Scalable, simple and gentle CTAB and chloroform based DNA precipitation method
  
• Cost effective

  Applications
  PCR, Real-Time PCR, Southern Blotting, Mapping and RFLP

  Caution
  IBI Plant Isolate contains irritants. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

  Additional Requirements
  Mortar and pestle, 1.5 mL microcentrifuge tubes or 15 mL centrifuge tubes, absolute ethanol for preparing 70% ethanol in water, chloroform, isopropanol, TE buffer or ddH2O

  Components and Storage

  Item	Product	Volume	RNase A (50mg/mL)	Shipping	Storage
  IBI Plant Isolate & RNase A	IB47610	4 mL	N/A	Room Temperature	Plant Isolate Dry at room temperature (15-25°C) for up to 1 year   RNase A 4°C for extended periods
  	IB47611	100 mL	50 µL
  	IB47612	200 mL	100 µL

Bullseye PREMIUM HS-Taq DNA Polymerase

Store at -20°C. For in-vitro laboratory use only

General Description

Bullseye HS Taq DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Taq DNA Polymerase into the reaction.

10X HS Buffer I

Tris-HCl, pH 8.5 (NH4)2S04, 15 mM MgCl2, 1% Tween 20.

10X HS Buffer II

An optimized buffer with a balanced ammonium/potassium concentration. May improve results with more complicated PCR reactions such as multiplex PCR.

Tris-HCl pH 8.7, Balanced KCl/(NH4)2S04, 15 mM MgCl2, 1% Tween 20.

HS Taq Storage Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20®, 0.5% NP40, 50% glycerol.

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Quality Control

Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of TEMPase DNA Polymerase.

Suggested Protocol using HS Taq DNA Polymerase

This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

• 15 mM MgCl2 is present in the 10X HS Buffer I and II. However, in some applications, more than 1.5mM MgCl2 is needed. For this reason, 25mM MgCl2 is included with the kit. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

  1. Thaw 10X HS Buffer I or/and 10X HS Buffer II, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

  2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

Table 1. Reaction components (master mix & template DNA) 

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
• Tube volume: 800µl
• Dialysis volume: 50-800µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 1cm x 0.5cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification.

Looking for other alternatives?

Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits

Key Features

• Amplification of multiple DNA targets with high GC content
• High specificity, sensitivity and product yield
• Diminished formation of non-specific product
• Detection of low copy number targets

Kit Components

• HS DNA Polymerase in Storage Buffer
• 5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
• mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20,
• 0.5% NP40, 50% glycerol.
• 4x GC Buffer I
• Optimized buffer components, 6 mM MgCl
• 4x GC Buffer II
• Optimized buffer components, 6 mM MgCl
• MgCl
• 25 mM MgCl in PCR grade water

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
• Tube volume: 3ml
• Dialysis volume: 0.1-3ml
• Min. sample size for extraction: 20µg
• Max. gel slice: 2cm x 1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

Bullseye PREMIUM HS-Taq DNA Polymerase Master Mix with HS Buffer I

Store at -20°C. For in-vitro laboratory use only

Bullseye HS Taq Polymerase, 2X Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye HS Taq Polymerase Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer I. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.

Bullseye HS Taq Polymerase Mix is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Bullseye" HS Taq Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of HS Taq Pol, 2x Mix

Tris-HCl pH 8.5, (NH4)2S04, 3.0mM MgCl2, 0.2% Tween 20Ã, 0.4 mM dNTPs, 0.2 units/uL HS Hot Start DNA Polymerase Stabilizer

Suggested Protocol using HS Taq Pol, 2x Mix

This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.

• Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
• The table below shows the reaction set up for a final volume of 50 mL.
• Important: Mix the solutions completely before use to avoid localized concentrations of salts.

  1. Set up each reaction as follows:

The Total RNA MINI and MAXI kits (Plant) provide a simple and fast method to isolate total RNA from plant tissue and cells. Samples are ground in liquid nitrogen and filtered to remove debris. In the presence of a binding buffer and chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix of the spin column. The optional DNase treatments can remove DNA residues and the contaminants can be washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation, and the entire procedure can be completed within 60 minutes. The purified total RNA is ready for RT, RT-PCR, Real Time PCR and northern blotting.

• Sample: Gram (+) positive and Gram (-) negative bacterial cells
• Yield: Up to 60 µg of RNA - (1 x 10 Escherichia coli: 40-45 µg, 1 x 10 Bacillus subtilis: 50-55 µg)
• Convenient: Includes Lysozyme and Bacteria Lysis Buffer
• Format: RNA spin columns
• Operation Time: Within 30 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months, Lysozyme should be stored at -20°C for extended periods

The rBAC Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative and Gram (+) positive bacteria. The provided Lysozyme and Bacteria Lysis Buffer will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Detergents and chaotropic salt are used to further lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and is ready for use in a variety of subsequent reactions.

Quality Control
The quality of the rBAC Mini RNA Bacteria Kit is tested on a lot-to-lot basis by isolating RNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

*Lysozyme should be stored at -20°C for extended periods. Add Lysozyme to Bacteria Lysis Buffer immediately prior to use. Once Lysozyme is mixed with Bacteria Lysis Buffer, the solution can be stored for 2 weeks at 4°C.

**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

• Sample: Variety of yeast and other fungus species
• Yield: Up to 30 µg of RNA (5 x 107 Saccharomyces cerevisiae: 20 µg)
• Convenient: Includes Sorbitol Buffer to reduce sample preparation time
• Format: RNA spin columns
• Operation Time: Within 70 minutes
• Elution Volume: 50-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 9 months

The rYeast Total RNA Mini Kit was designed for total RNA purification from yeast and a wide variety of other fungus species. Sorbitol Buffer is included with the kit to reduce sample preparation time and minimize hands on time. Detergents and chaotropic salt are used to lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. The entire procedure can be completed within 70 minutes and the purified RNA is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis.

Quality Control
The quality of the rYeast Total RNA Mini Kit is tested on a lot-to-lot basis by isolating RNA from Saccharomyces cerevisiae (5 x 10  ) harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µL aliquot of purified RNA from a 50 µL eluate is analyzed by electrophoresis on a 0.8% agarose gel.

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

IBI's Viral Nucleic Acid Extraction Kit was specifically designed for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids and the supernatant of viral-infected cell cultures. These kits are recommended for parallel purification of viral DNA including HBV and CMV, as well as viral RNA including HCV, HIV and HTLV. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assays.

IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in IBI Isolate without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Quality Control
IBI Tri-Isolate is tested on a lot-to-lot basis. An Escherichia coli (1 x 109) culture (OD600=1.3, 1 mL) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by IBI Isolate homogenization. RNA is then purified using a spin column procedure. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages

• Purify total RNA within 15 minutes without chloroform phase separation or isopropanol RNA precipitation
• Up to: 200 µL (blood, buffy coat, serum, plasma), 5 x 106 (cultured cells), 10-50 mg (tissue), 1 x 109 (bacteria cells)
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays,
• Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Components and Storage

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer prior to initial use.