IBI High-Speed Mini Plasmid Kit

The High-Speed Mini Plasmid Kit isolates plasmid DNA from 1-5ml cultures using alkaline lysis and RNase treatment, ensuring minimal genomic DNA/RNA contamination.

Additional Details

The High-Speed Mini Plasmid Kits by IBI Scientific are designed to process a sample size of 1-4 mL of bacterial culture, yielding 20-30 µg for high-copy plasmids and 3-10 µg for low-copy plasmids. With an operation time of 30 minutes or less and a binding capacity of up to 30 µg, these kits offer a rapid and efficient solution for plasmid DNA extraction. Check out our basic guide for plasmid kits for more information.

Technical Details

In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to the glass-fiber matrix in the spin column. The contaminants are washed off with an ethanol-based wash buffer. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol plasmid. Typical yields are 20-30 µg for high-copy numbered plasmids or 3-10 µg for low-copy numbered plasmids. The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, or sequencing reactions. The entire procedure can be completed in under 30 minutes.

Quality Control

The quality of the High-Speed Plasmid Mini Kit is tested on a lot-to-lot basis, by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid p Bluescript (A600>2U/mL). Following the purification process, a yield of more than 20 µg is expected and the ratio of A260/A280 is between 1.7-1.9. The purified plasmid (1 µg) is used in EcoRI digestion and checked by electrophoresis.

Physical Specifications

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

• Sample: 1-7 mL of cultured bacterial cells
• Yield: Up to 50 µg of pure plasmid DNA
• Format: Plasmid spin column
• Operation Time: Within 15 minutes
• Elution Volume: 30-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Kit Components

*For IB47171 and IB47172 add provided RNase A to PD1 Buffer then mix by shaking for a few seconds. Check the box on the bottle. PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months. For IB47170 samples, RNase A was already added to PD1.

**If precipitates have formed in PD2 Buffer, warm the buffer in a 37°C water bath, followed by gentle shaking to dissolve.

***Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

The Fast Ion Plasmid Maxi Kit (Endotoxin Free) uses pre-packed anion exchange resin columns to purify plasmid DNA from 100-250 ml of bacterial cultures. Modified alkaline lysis method (1) and RNase treatment are used for obtaining clear cell lysate with minimal genomic DNA and RNA contaminants. Once the plasmid DNA has been bound to the column, the contaminants can be washed off using the wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR and in-vitro transcription.

The 96-Well Genomic DNA Kits are designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood and a variety of animal tissues or cells. This method uses Proteinase K and a chaotropic salt to lyse cells and degrade proteins. DNA in the chaotropic salt is bound by the glass fiber matrix of each well. Once any contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. The entire procedure can be completed in 1 hour without phenol extraction or alcohol precipitation. These kits can be used for manual filtration or with robotic handling systems, and purified DNA with approximately 20-30kb is suitable for PCR or other enzymatic reactions.

The Genomic DNA Mini Kit for Plants provide a quick and easy method for purifying total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from plant tissue. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require DNA phenol extraction or alcohol precipitation, and can be completed in less than 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting and RFLP.

The Genomic DNA Mini Kit for Tissue was designed specifically for purifying total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed within 1 hour without phenol/chloroform extraction or alcohol precipitation. The expected yield of genomic DNA is up to 50µg and the purified DNA (with approximately 20-30 Kb) is suitable for use in PCR or other enzymatic reactions.

The Genomic DNA Kit for Blood or Cultured Cells provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 6 µ g from 200 µ l of whole human blood and up to 50µ g of DNA from 200µ l of buffy coat. Purified DNA, with approximately 20-30 Kb, is suitable for use in PCR or other enzymatic reactions.

MagBio HighPrep™ Viral/Bacterial DNA/RNA Kit

, Beads

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Dilution Solution (50 mL)
 
For hydrogel concentration adjustment with the high concentration hydrogels.

• Type 1 - Contains sucrose to maintain the best osmolarity.
• Type 2 - Sucrose-free formulation for cells sensitive to the sugar level in the medium.

VitroGel® Dilution Solution is a ready-to-use solution to be used with the VitroGel® High Concentration hydrogels to adjust the hydrogel concentration for different hydrogel strength.

The solution is room temperature stable with a neutral pH, which maintains a good physiological condition for hydrogel formation and can accelerate the hydrogel formation in combination with the cell medium. The Dilution Solution can be used to prepare a thin gel coating plate or injectable hydrogel with the high concentrations hydrogels.

Specifications

• Ready-to-use to adjust the concentration of VitroGel® High Concentration hydrogels
• Neutral pH
• Room temperature stable
• Transparent
• Compatible with VitroGel® system for 2D & 3D cell culture and hydrogel injection
• Ships and store at room temperature
• Size: 50 mL

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
 
VitroGel® Angiogenesis Assay HC Kit
 
Tunable hydrogel system for 2D gel coating and 3D culture of angiogenesis tube formation, invasion, and animal injection.

• Hydrogel Kit Only: VitroGel® AAK-HC hydrogel ( 1 mL), AAK Dilution Solution (10 mL)
• Type 1 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 1 (3 x 500 µL), AAK Supplement 2 (3 x 500 µL)
• Type 2 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 1 (6 x 500 µL)
• Type 3 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 2 (6 x 500 µL)

VitroGel® Angiogenesis Assay HC Kit

• Xeno-free functional hydrogel system supporting angiogenesis process
• Control and study the effect of the hydrogel mechanical properties on the angiogenesis process
• Multiple applications in one kit:  Tube formation, invasion, and animal injection
• Control the hydrogel properties:  Add your own growth factors and compare with positive control
• Harvest cells within 20 minutes at 37 °C without enzyme solution

VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating and 3D cell culture methods. The VitroGel® system is also good for animal injection for in vivo studies.

Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.

Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties.  Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.

There are two versions of VitroGel® Angiogenesis Assay Kits:

• VitroGel® Angiogenesis Assay Kit (Cat No. VHM06-K):  Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements.
• VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K):  Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.

Depending on the kit type, the tunable VitroGel Angiogenesis Assay HC Kit can contain the following components:

• VitroGel® AAK-HC, a tunable, xeno-free high concentration hydrogel.
• AAK Dilution Solution, for adjusting the hydrogel concentration
• AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for promoting cell attachment and growth.
• AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.
• All the components can be purchased separately.

Besides molecular cues, the VitroGel® Angiogenesis Assay HC Kit allows researchers to explore the effects of hydrogel mechanical properties on angiogenesis.  The high concentration VitroGel® AAK-HC hydrogel is room temperature stable and can be adjusted by simply mixing the hydrogel solution and dilution solution at different rations (recommend 1:1 to 1:5 v/v) to achieve different mechanical strengths. The diluted hydrogel solution can be directly mixed with supplement at 2:1 (v/v) ratio for hydrogel formation. Researchers can adjust the molecular cues of the hydrogel by adding the growth actors/inhibitors directly to the supplement before mixing with VitroGel® AAK-HC.  Cells cultured in this system can be further harvested easily with the VitroGel® Cell Recovery Solution.

Specifications 

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

ig BL21 (DE3)
Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.

Specifications
Competent cell type:                Chemically competent
Derivative of:                            BL21(DE3)
Species:                                   E. coli
Format:                                    Tubes
Transformation efficiency:         1.0 x 109 cfu/µg pUC19 DNA
Blue/white screening:               Yes
Shipping condition:                   Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
BL21(DE3) Chemically Competent Cells:             50 µl
DNA (or pUC19 Control, 10 pg/µl):                         1 µl
Recovery Medium:                                                 1 ml

Contents & Storage
BL21(DE3) Competent Cells:                -80 °C
pUC19 Control DNA:                            -20 °C
Recovery Medium:                                  4 °C

Genomic Features
BL21(DE3) chemically competent cells have the following features:

• Widely used host background
• T7 Expression Strain
• Deficient in both lon (1) and ompT proteases
• Resistant to phage T1 (fhuA2)
• B Strain

Genotype
F-ompT hsdS(rB- mB-) gal dcm (DE3)

General Guidelines
Follow these guidelines when using BL21(DE3) chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Transformation Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl)
• DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
• Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on pre-warmed selection plates.
• Incubate the plates overnight at 37 °C. 

ig 5-Alpha
Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.

Number of Colonies per Transformation Test*

• Up to 2x higher efficiency than any competitor
• Exact same Genotype as NEB's 5-Alpha Cells

Specifications
Competent cell type:        Chemically competent
Derivative of:                    5-alpha
Species:                            E. coli
Format:                             Tubes
Transformation efficiency:      1 x 109 cfu/µg pUC19 DNA
Blue/white screening:           Yes
Shipping condition:               Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
ig 5-alpha chemically competent cells:  50 µl
DNA (or pUC19 Control, 10 pg/µl):           1 µl
Recovery medium:                                   1 ml

Contents & Storage
ig 5-alpha competent cells:   -80 °C
pUC19 control DNA:                 -20 °C
Recovery medium:                       4 °C

Genotype
80 (lacZ)M15 fhuA2 (argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17

General Guidelines
Follow these guidelines when using ig 5-alpha chemically competent cells.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping. 4) Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on Pre-warmed selection plates.
• Incubate the plates overnight at 37 °C.