Dextrose (D-Glucose)

• Appearance (Color): White to Off-White
• Appearance (Form): Crystalline Powder
• Purity: ≥ 99.5%
• Solubility (2 g / 15 mL): Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Conductivity: ≤ 20 uS / cm
• Specific Rotation: 52.6° to 53.2°
• Water: ≤ 1.0%
• Lead: ≤ 0.5 ppm
• Sulfates: ≤ 200 ppm
• Chloride: ≤ 125 ppm
• Sulfated Ash: ≤ 0.1%
• Calcium: ≤ 200 ppm
• Endotoxins: ≤ 1.25 EU/g
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® – Bioink Starter Kits
 
VitroINK® (3 mL) + Full Mixing Kit with Dispenser

VitroINK® Bioink Starter Kit contains both bioink and the complete mixing kit to prepare the cell bioink mixture for 3D bioprinting. The kit includes choice of bioink + the mixing kit:

• VitroINK 3D (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK RGD (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK COL (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK MMP (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK IKVAV (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK YIGSR (3 mL) + VitroINK® Mixing Kit with Dispenser

The VitroINK® Mixing Kit includes a dispenser, 1 mL syringe, 3 mL syringe, connectors and mixing head to get you started.

VitroINK® is a ready-to-use tunable bioink modified with functional ligands, and cell adhesive peptide, promoting cell attachment and cell-matrix interactions. This RGD modified bioink can enhance cell adhesion, proliferation, motility/migration, and differentiation in different applications.

VitroINK® Mixing Kit was designed to provide a robust mixing of bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. Put the VitroINK® and cell suspension in the mixer and gently press the mixture through the connector and mixing head to your bioprinter cartridge. Wait 10-20 minutes for the mixture to stabilize before printing. There is no UV,  no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Simply adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

Contents and Specifications

VitroINK®

• Xeno-free tunable modified bioink.
• Ready-to-use at room temperature
• No UV, temperature/pH curing, or chemical cross-linking required
• Neutral pH
• Transparent. Excellent visibility after printing and cell culture
• Pre-mix with cells using the included VitroINK® Mixing Kit
• Ships room temperature. Store at 2-8°C
• Size: 3 mL

VitroINK® Mixing Kit

• Complete mixing kit.  Includes:
• (1) 1 mL syringe
• (1) 3 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 3:1 or 10:1 mixing ratio
• Size: 3 mL

IBI DNA/RNA/Protein Extraction Kits

• Sample Size: up to 5×106 cultured animal cells/up to 25mg animal tissue /up to 500µl whole blood/up to 200µl biological fluids (serum, plasma)
• Expectant Yield: up to 9µg of genomic DNA/20µg of total RNA/120µg of protein from 1×106 of HeLa cells
• Operation Time: DNA/RNA purification within 25 minutes, protein precipitation within 50 minutes
• Elution Volume: 50  200µl (genomic DNA)/25  50µl (total RNA)
• Format: genomic DNA spin column and total RNA spin column

The IBI All Prep DNA RNA Protein Mini Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.

DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20  30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

The quality of the All Prep DNA RNA Protein Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1x106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.  The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.

Specifications

Sample: Cultured cells, animal tissue, whole blood & biological fluids
Format: Genomic DNA spin column and Total RNA spin column.
Yield: up to 9ug of Genomic DNA / 20ug of Total RNA / 120ug of Protein from 1 x 106 HeLa cells.
Operation Time: Within 25 minutes
Elution volume: 50  200ul for Genomic DNA / 25  50ul for Total RNA.
Simultaneous extraction of Genomic DNA and Total RNA from cultured cells, animal tissues, whole blood and biological fluids.

• Used for agarose electrophoresis of DNA, RNA or nucleic acids
• Contains 3 tracking dyes and 15% Ficoll in a special Tris dye

• Light blue - around 4000bp in 1% agarose
• Indigo - around 600bp in 1% agarose
• Magenta - around 150bp in 1% agarose

• DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

• Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
• RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
• DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

• Tracks the migration progression of your sample during polyacrylamide electrophoresis
• Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
• DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

• Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
• CAS Number: 2650-17-1
• DNase/RNase/Protease free

IB74040
Bromophenol Blue

• Tracking dye in electrophoretic separations
• Migrates around 0.5kb
• CAS Number: 115-39-9
• Purity: >98.0%

MagBio HighPrep™ Viral DNA/RNA Kit

, Beads

• Sample: Cultured cells
• Yield: High yield, High quality DNA (A260/A280 = 1.8-2.0)
• Format: Scalable DNA precipitation method
• Kit Storage: Dry at room temperature (15-25°C) for up to 2 years, RNase A should be stored at 4°C for extended periods

The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Quality Control
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 μg with an A260/A280 ratio of 1.8â2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Components and Storage
Cell Lysis Buffer, Protein Removal Buffer, DNA Hydration Buffer should be stored dry at room
temperature (15-25°C) for up to 1 year. RNase A should be stored at 4°C for extended periods.

• Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
• Yield: 5-30 µg
• Format: Spin column
• Operation time: Within 20 minutes

Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.

Quality Control 
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.

Caution 
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.

Introduction
IBI Isolate is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting.

Quality Control
IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µL from a 50 µL eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages
 Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
 Sample: up to 300 µL (blood, buffy coat, serum, plasma), up to 5 x 106 (cultured cells), 50-100 mg (tissue)
 Scalable
 Format: phenol, chloroform and guanidine isothiocyanate

Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Additional Requirements
RNA Extraction: chloroform, isopropanol, 70% ethanol, RNase-free Water, 1.5 mL microcentrifuge tubes (RNase-free) DNA Extraction: chloroform, absolute ethanol, 70% ethanol, sodium citrate/ethanol solution (0.1 M sodium citrate in 10% ethanol, pH 8.5), 8 mM NaOH solution or TE Buffer pH 8.5, 1.5 mL microcentrifuge tubes

Components and Storage
IBI Isolate is shipped at room temperature and can be stored dry at 2°C to 25°C for up to 9 months.

The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial, and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1.5 hours. The isolated total DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping, and RFLP.

• Reduces formation of non-specific products
• Improves results from multiplex reactions
• Essential for low copy number
• Buffer II: potassium/ammonium buffer for multiplex reactions
• Activated at elevated temperatures
• Gives higher specificity and greater yields than standard Taq
• Chemical moiety is attached to the enzyme at the active site and renders it inactive at room temperature; is cleaved during a 15 min. heat activation step
• Prevents mispriming during setup and the first ramp of thermal cycling

MINI Flex Tube Kit

MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
• Tube volume: 250µl
• Dialysis volume: 10-250µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 0.4cm x 1.1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

This brand is being discontinued.

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!

Bullseye PREMIUM PR DNA Polymerase

• Provides higher fidelity than standard Taq DNA Polymerase
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity

Store at -20°C. For in-vitro laboratory use only

Bullseye PREMIUM PR DNA Polymerase is a thermostable enzyme with proofreading ability, which can be used in primer extension reactions and other molecular biology applications. PR Polymerase exhibits both 5'-3' DNA polymerase activity and 3'-5' proofreading exonuclease activity. It is recommended for applications, which require extremely high fidelity or blunt ending.

Optimal reaction conditions are achieved by using the 10x Ammonium buffer containing MgCl2 provided with the enzyme. 25 mM MgCl2 is also included separately, in case a higher MgCl2 concentration is required for a specific reaction.

Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2SO4, 1% Tween20,
15mM MgCl2

PR Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 0.1% NP40, 0.1% Tween-20.

Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of PR DNA Polymerase.

Suggested Protocol using PR DNA Polymerase
 
This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, will produce satisfactory results. Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

Table 1. Reaction components (master mix and template DNA)

Table 2. MgCl2 concentration in a 50 uL reaction

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.

4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturer's instructions. PR is a proofreading enzyme and requires an extension time of 1-2 min/kb. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.