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COVID-19 genesig® Real Time PCR assay | 96 Tests
 
  • Lyophilized components to ship without cold chain
  • Highly specific detection profile and high priming efficiency
  • Rapid detection of COVID-19 and does not detect other related coronavirus strains


Design of our COVID-19 was an intricate process using a dedicated team of expert bioinformaticians. Finding the right sequence is critical for not only assay specificity, but also sensitivity which can be inhibited by primer-dimer formation. These ultimately reduce the assay's ability to detect SARS-CoV-2 accurately. However, the right design will mitigate these risks and ensure a specific, sensitive and accurate test, as we have done.


Our surveillance program is in place to track the mutation of the virus. Our expert bioinformaticians analyze daily published virus sequences to ensure our test maintains a 100% detection profile. Each new mutation presents a risk to any COVID-19 kit detection and false negatives without surveillance programs in place. As of the 17th July 2020 our COVID-19 test has a 100% detection profile with the 42,655 full length, good quality SARS-CoV-2 sequences published on the GISAID-EpiCoV database.


Ensuring your COVID-19 test is stable during transit and storage is vital to prevent reagent degradation. Within our COVID-19 genesig® Real Time PCR assay we provide oasig components that are provided freeze dried which stabilizes the active components. This enables the reagents to be shipped at ambient temperature. This simplifies the logistics of shipping and is cost effective. If you're in a sophisticated laboratory or a mobile field hospital we can supply complete qPCR packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.


The COVID-19 genesig® Real Time PCR assay was granted Emergency Use Authorization Only by the United States Food and Drug Administration (FDA).

Item#:
ASCODVID19

MagBio MagQuant™ Plus DNA V2


Magnetic bead based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications


Applications

DNA quantitation and normalization of DNA concentration for:

  •     Next Gen Sequencing library construction
  •     Amplicon normalization
  •     Genomic DNA normalization
  •     PCR, qPCR

Benefits

  •     Rapid and reliable quantitation and normalization of DNA
  •     No centrifugation step, no filtration step
  •     Eliminates the need for standard curve
  •     Allowing the processing of pool DNA samples from various sources
  •     Equilizing input genomic DNA concentration for DNA libray construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.

The MagQuant™ Plus DNA V2 Kit utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization.  The DNA normalization occurs during the purification process.  Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant™ Plus DNA V2‘s proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.
Item#:
ASMAGQUANTPDNA2

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® Mixing Kit – Complete Pack
 
Mix VitroINK® bioink with cells at 3:1 and 10:1 ratios. Full mixing kit with Dispenser and single-use components.

Mixing cells with the bioink is a critical step for 3D bioprinting. Because of the differences in viscosity of the cell suspension and bioink, the traditional manual mixing methods create a lot of air bubbles and non-uniform mixture which leads to the unstable printed structure, difficulty for cell observation and affect cell viability.

The VitroINK® Mixing Kit was designed to provide a robust mixing of the bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. To prepare the bioprinter cartridge, the VitroINK® and cell suspension are placed in the mixer and is dispensed through the connector and mixing head. Wait 10-20 minutes for the mixture to stabilized and the cartridge is ready for printing. There is no UV, no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

The syringe, connector and mixing head are disposable for one-time use. Order the sterilized replacement mixing kit components to ensure the robust results for every single mixing.

 

Kit Contents and Specifications

  • Disposable mixing kit components for VitroINK bioink:
    • (1) Dispenser
    • (1) 1 mL syringe
    • (1) 3 mL syringe
    • (1) connector and tubing
    • (1) mixing head
  • Sterile
  • Use for 3:1 or 10:1 mixing ratio
  • Ships room temperature. Store at room temperature.



TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

 

Item#:
ASVITROINKMK

MagBio HighPrep™ PCR Clean-up System


 Magnetic beads based reagent for manual and automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and reliable amplicon size selection.
 
Applications

Post-PCR and post-enzymatic reaction clean-up used for/during:
  •     NGS library preparation
  •     Amplicon size selection for NGS
  •     Microarrays
  •     PCR
  •     Restriction enzyme digestions, adapter ligations
  •     Cloning
Benefits

  •     Rapid and reliable post-PCR and post enzymatic reaction clean-up
  •     Efficient wash from contaminants
  •     High recovery of amplicons >100bp
  •     Uniform fragments size distribution
  •     Adaptable to high throughput liquid handling workstations

The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for an efficient purification of DNA fragments and tight size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger  sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. This protocol can be used for manual procedure as well as as a guideline for adapting the kit to automatic liquid handling instruments. The HighPrep™ PCR Clean-up Reagent is perfectly suited for amplicon size selection, and NGS library preparation for sequencing as well as for post PCR clean-up of shorter amplicons or restriction enzyme digestion products.
Item#:
ASHIHPRPPCRCUS


Dextrose (D-Glucose)

  • Appearance (Color): White to Off-White
  • Appearance (Form): Crystalline Powder
  • Purity: ≥ 99.5%
  • Solubility (2 g / 15 mL): Clear and Colorless
  • Identity (FTIR): Conforms to Structure
  • Conductivity: ≤ 20 uS / cm
  • Specific Rotation: 52.6° to 53.2°
  • Water: ≤ 1.0%
  • Lead: ≤ 0.5 ppm
  • Sulfates: ≤ 200 ppm
  • Chloride: ≤ 125 ppm
  • Sulfated Ash: ≤ 0.1%
  • Calcium: ≤ 200 ppm
  • Endotoxins: ≤ 1.25 EU/g
  • RNase: None Detected
  • DNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.   
 

Item#:
ASDEXANHY

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® – Bioink Starter Kits
 
VitroINK® (3 mL) + Full Mixing Kit with Dispenser

VitroINK® Bioink Starter Kit contains both bioink and the complete mixing kit to prepare the cell bioink mixture for 3D bioprinting. The kit includes choice of bioink + the mixing kit:

  • VitroINK 3D (3 mL) + VitroINK® Mixing Kit with Dispenser
  • VitroINK RGD (3 mL) + VitroINK® Mixing Kit with Dispenser
  • VitroINK COL (3 mL) + VitroINK® Mixing Kit with Dispenser
  • VitroINK MMP (3 mL) + VitroINK® Mixing Kit with Dispenser
  • VitroINK IKVAV (3 mL) + VitroINK® Mixing Kit with Dispenser
  • VitroINK YIGSR (3 mL) + VitroINK® Mixing Kit with Dispenser

The VitroINK® Mixing Kit includes a dispenser, 1 mL syringe, 3 mL syringe, connectors and mixing head to get you started.

VitroINK® is a ready-to-use tunable bioink modified with functional ligands, and cell adhesive peptide, promoting cell attachment and cell-matrix interactions. This RGD modified bioink can enhance cell adhesion, proliferation, motility/migration, and differentiation in different applications.

VitroINK® Mixing Kit was designed to provide a robust mixing of bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. Put the VitroINK® and cell suspension in the mixer and gently press the mixture through the connector and mixing head to your bioprinter cartridge. Wait 10-20 minutes for the mixture to stabilize before printing. There is no UV,  no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Simply adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

 

Contents and Specifications

VitroINK®

  • Xeno-free tunable modified bioink.
  • Ready-to-use at room temperature
  • No UV, temperature/pH curing, or chemical cross-linking required
  • Neutral pH
  • Transparent. Excellent visibility after printing and cell culture
  • Pre-mix with cells using the included VitroINK® Mixing Kit
  • Ships room temperature. Store at 2-8°C
  • Size: 3 mL


VitroINK® Mixing Kit

  • Complete mixing kit.  Includes:
    • (1) 1 mL syringe
    • (1) 3 mL syringe
    • (1) connector and tubing
    • (1) mixing head
  • Sterile
  • Use for 3:1 or 10:1 mixing ratio
  • Size: 3 mL

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

  

Item#:
ASVITROINKSK

    IBI DNA/RNA/Protein Extraction Kits

  • Sample Size: up to 5×106 cultured animal cells/up to 25mg animal tissue /up to 500µl whole blood/up to 200µl biological fluids (serum, plasma)
  • Expectant Yield: up to 9µg of genomic DNA/20µg of total RNA/120µg of protein from 1×106 of HeLa cells
  • Operation Time: DNA/RNA purification within 25 minutes, protein precipitation within 50 minutes
  • Elution Volume: 50  200µl (genomic DNA)/25  50µl (total RNA)
  • Format: genomic DNA spin column and total RNA spin column

The IBI All Prep DNA RNA Protein Mini Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.

DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20  30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

The quality of the All Prep DNA RNA Protein Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1x106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.  The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.

Specifications

Sample: Cultured cells, animal tissue, whole blood & biological fluids
Format: Genomic DNA spin column and Total RNA spin column.
Yield: up to 9ug of Genomic DNA / 20ug of Total RNA / 120ug of Protein from 1 x 106 HeLa cells.
Operation Time: Within 25 minutes
Elution volume: 50  200ul for Genomic DNA / 25  50ul for Total RNA.
Simultaneous extraction of Genomic DNA and Total RNA from cultured cells, animal tissues, whole blood and biological fluids.

Item#:
ASDNARNAKIT40
While Supplies Last!


These items have been discontinued. Contact us for more options.

 
IB01010
6X Loading Dye

    • Used for agarose electrophoresis of DNA, RNA or nucleic acids
    • Contains 3 tracking dyes and 15% Ficoll in a special Tris dye
      • Light blue - around 4000bp in 1% agarose
      • Indigo - around 600bp in 1% agarose
      • Magenta - around 150bp in 1% agarose
    • DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

  • Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
  • RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
  • DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

    • Tracks the migration progression of your sample during polyacrylamide electrophoresis
    • Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
    • DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

    • Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
    • CAS Number: 2650-17-1
    • DNase/RNase/Protease free

IB74040
Bromophenol Blue

  • Tracking dye in electrophoretic separations
  • Migrates around 0.5kb
  • CAS Number: 115-39-9
  • Purity: >98.0%
Item#:
ASGELLDDYES

MagBio HighPrep™ Viral DNA/RNA Kit


Magnetic beads based kit for rapid isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids. 
OPTIMIZED FOR ISOLATION OF SARS-CoV-2 RNA


Applications

Viral RNA and DNA isolation for:

  •     RT-qPCR; RT-PCR, PCR
  •     One-Step RT-qPCR
  •     Virus detection, genotyping
  •     Viral load monitoring,

Benefits

  •     OPTIMIZED FOR ISOLATION OF SARS-CoV-2 RNA
  •     Rapid and reliable purification of viral nucleic acids
  •     Adaptable to various automated liquid handling workstations
  •     No toxic organic solvents

The HighPrep™ Viral DNA/RNA kit is designed for rapid and reliable isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
Item#:
ASHIHPRPVIRDNRNK
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  • Sample: Cultured cells
  • Yield: High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Format: Scalable DNA precipitation method
  • Kit Storage: Dry at room temperature (15-25°C) for up to 2 years, RNase A should be stored at 4°C for extended periods

The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Quality Control
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 μg with an A260/A280 ratio of 1.8â2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Components and Storage
Cell Lysis Buffer, Protein Removal Buffer, DNA Hydration Buffer should be stored dry at room
temperature (15-25°C) for up to 1 year. RNase A should be stored at 4°C for extended periods.


gPURE DNA Isolation Kit

IB47430

IB47431

IB47432

Number of Cells Processed Per Kit

2 x 10

6 x 10

6 x 10

Cell Lysis Buffer

3 mL

100 mL

1000 mL

Protein Removal Buffer

1 mL

40 mL

400 mL

DNA Hydration Buffer
(10 mM Tris-HCl, 1mM EDTA, pH 8.0)

1 mL

50 mL

500 mL

RNase A (10 mg/mL)

25 µL

550 µL

5 mL

Cell Number

0.5-1 x 10 

3-5 x 10 

3.5 x 10

Tube Size

1.5 mL

1.5 mL

15 mL

Cell Lysis Buffer

150 µL

600 µL

6 mL

RNase A (10 mg/mL)

1 µL

3 µL

30 µL

Protein Removal Buffer

50 µL

200 µL

2 mL

Isopropanol

150 µL

600 µL

6 mL

70% Ethanol

150 µL

600 µL

6 mL

DNA Hydration Buffer

50 µL

100 µL

200 µL

Item#:
ASDNARNAKIT22
  • Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
  • Yield: 5-30 µg
  • Format: Spin column
  • Operation time: Within 20 minutes

Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.

Quality Control 
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

Kit

IB47300

IB47301

IB47302

IB47303

RB Buffer

2 mL

30 mL

60 mL

130 mL

W1 Buffer

2 mL

30 mL

50 mL

130 mL

Wash Buffer*
(Add EtOH)

1 mL
(4 mL)

12.5 mL
(50 mL)

25 mL
(100 mL)

50 mL x 2
(200 mL x 2)

RNase-free H2O

1 mL

6 mL

15 mL

30 mL

RB Column

4 pcs

50 pcs

100 pcs

300 pcs

Filter Column

4 pcs

50 pcs

100 pcs

300 pcs

2 mL Collection Tubes

8 pcs

100 pcs

200 pcs

600 pcs

Micropestle

4 pcs

50 pcs

100 pcs

300 pcs

*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.

Caution 
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.

Item#:
ASDNARNAKIT21

Introduction
IBI Isolate is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting.

Quality Control
IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µL from a 50 µL eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages
 Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
 Sample: up to 300 µL (blood, buffy coat, serum, plasma), up to 5 x 106 (cultured cells), 50-100 mg (tissue)
 Scalable
 Format: phenol, chloroform and guanidine isothiocyanate

Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Additional Requirements
RNA Extraction: chloroform, isopropanol, 70% ethanol, RNase-free Water, 1.5 mL microcentrifuge tubes (RNase-free) DNA Extraction: chloroform, absolute ethanol, 70% ethanol, sodium citrate/ethanol solution (0.1 M sodium citrate in 10% ethanol, pH 8.5), 8 mM NaOH solution or TE Buffer pH 8.5, 1.5 mL microcentrifuge tubes

Components and Storage
IBI Isolate is shipped at room temperature and can be stored dry at 2°C to 25°C for up to 9 months.

IB47600

IBI Isolate Total DNA & RNA Extraction Reagent, Sample 4 mL

IB47601

IBI Isolate Total DNA & RNA Extraction Reagent, 100 mL

IB47602

IBI Isolate Total DNA & RNA Extraction Reagent, 200 mL

Item#:
ASDNARNAKIT24
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