This brand is being discontinued, please contact us for other options.
 

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Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits

General Description
Bullseye GC HS 2x Master Mix II is an all-in-one 2x master mix containing HS DNA polymerase, GC Buffer II, enhancer, dNTPs and MgCl. Simply mix GC HS 2x Master Mix II with primers,template and water and you are ready to carry out successful primer extensions. HS DNA Polymerase is a modified form of Bullseye Taq DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases.

Key Features

• For amplification of DNA targets with high GC content
  
• Convenient reaction set-up at room temperature
  
• High specificity, sensitivity and product yield
  
• Detection of low abundance targets
  
• Diminished formation of non-specific product

  Composition of GC HS 2x Master Mix I
  

• HS DNA Polymerase
  
• Optimized buffer components, 3.0 mM MgCl
  
• dNTPs Enhancer

Storage and Stability

• The unopened kit is stable at -20°C for 1 year

MagBio HighPrep™ DTR Clean-up System

IBI MIDI I-Blue Plasmid Kit

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

• Sample: 1-7 mL of cultured bacterial cells
• Yield: Up to 50 µg of pure plasmid DNA
• Format: Plasmid spin column
• Operation Time: Within 15 minutes
• Elution Volume: 30-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control

This brand is being discontinued.
 

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Bullseye Pre-stained Protein Ladder, 10-180 kDa

• Ready to use - no boiling necessary
• Stable at ambient temperature
• 2 reference bands - red at ~75kDa and green at ~25kDa
• Use 5-15µl, depending on the size of the wells
• No freeze-thaw involved
• Broad range ladder

Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.

The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.

• Contents: 0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.
• Usage: For each application, 5-15µl is needed, depending on the size of the wells.
• Quality Control: Tested in polyacrylamide gel electrophoresis and Western Blotting.
• Storage: -20 °C.

MagBio MagCTL Collection Tube

IBI DEPC-Treated, Nuclease-Free Water is ideally suited for all applications in molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and specifically for all RNA assays.

IBI DEPC-Treated Water is manufactured under stringent conditions. The purification process for this product includes continuous
deionization, reverse osmosis, UV-treatment, 0.1µm filtration, followed by steam sterilization in an autoclave. The product is re-autoclaved
to ensure the breakdown of DEPC and release of carbon dioxide that may cause the pH to shift.

Citric Acid, Anhydrous

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals or Crystalline Powder
• Purity: ≥ 99.5%
• Identification (FTIR): Conforms to Structure
• Water: ≤ 0.3%
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Oxalate: ≤ 100 ppm
• Sulfate: ≤ 150 ppm
• Chloride: ≤ 50 ppm
• Barium: Passes Test
• Residue on Ignition: ≤ 0.05%
• Readily Carbonizable Substances: Passes Test
• Bacterial Endotoxins: ≤ 0.5 IU/mg
• DNase: None Detected
• RNase: None Detected 

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® 3:1 Mixing Component Pack

IBI Small DNA Fragment

Specifications
 

Sample	Up to 300 mg agarose gel slice | Up to 100 uL PCR product or other enzymatic reaction
Format	Spin columns
Operation	Centrifuge/Vacuum Manifold
Binding Capacity	10 ug DNA
Expectant Yield	80-90% for gel extraction | 90-95% for PCR clean-up
Operational Time	15 min. for PCR clean-up | 20 min. for gel extraction
Applications	PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

 

IBI Small DNA Fragment

Specifications
 

Sample	Up to 300 mg agarose gel slice | Up to 100 uL PCR product or other enzymatic reaction
Format	Spin columns
Operation	Centrifuge/Vacuum Manifold
Binding Capacity	10 ug DNA
Expectant Yield	80-90% for gel extraction | 90-95% for PCR clean-up
Operational Time	15 min. for PCR clean-up | 20 min. for gel extraction
Applications	PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

 

MagBio HighPrep™ Blood & Tissue DNA Kit

•     High-quality DNA from blood and tissue samples
•     No organic extraction or alcohol precipitation
•     Complete removal of contaminants and inhibitors
•     Adaptable to various automated workstations

, Beads

MagBio HighPrep™ Viral RNA Kit

, Beads