PCR DNA Clean-up Kit

Specifications
 

Sample Size	Up to 50 uL of PCR product Up to 50 mg of agarose slice
Format	96-Well Plates
Operation	Centrifuge/Vacuum manifold
Binding Capacity	10 uG per well
DNA Size	50 bp - 10 kb
Recovery	80-90% for Gel Extraction 90-95% for PCR Clean-Up
Operation Time	30 min for PCR Clean-Up 40 min for Gel Extraction

 

MagBio HighPrep™ Insect DNA Kit

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• RNase & DNase Free Water A new process of purifying large quantity of DNase and RNase free water enables us to offer Molecular Grade Water at a price that any laboratory can afford. 
  
  
• No toxic agents such as DEPC are used in making the Molecular Grade Water; therefore, there is no chance of DEPC interfering with enzymatic reactions. 
  
  
• The Molecular Grade Water is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase and RNase contamination. 
  
  
• Molecular Grade Water can be used for making reagents and rinsing glass/plastic wares for DNA and RNA research. 
  
  
• Each lot is quality tested for the absence of RNase and DNase contamination. Test reports are attached with each container as an added assurance.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® 10:1 Mixing Component Pack
 
Single-use components for 10:1 mixing ratio for VitroINK® Mixing Kit. DISPENSER NOT INCLUDED

The VitroINK® 10:1 Mixing Component Kit includes a 1 mL syringe, connector and mixing head to replace the one-time-use components in the full VitroINK® Mixing Kit – Complete Pack (Item # IMK00-1) for 10:1 VitroINK® and cells mixing.

This package is sterilized and disposable for one-time use.

Kit Contents and Specifications

• Disposable mixing kit components for VitroINK® kit:
• (1) 1 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 10:1 mixing ratio
• Ships room temperature. Store at room temperature.

MagBio HighPrep™ RNA Elite Clean-up System

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Sucrose

• Appearance (Color): White
• Appearance (Form): Crystalline Powder
• Purity: ≥ 99.0%
• Identity (FTIR): Conforms to Structure
• Solubility (1M Aqueous): Clear and Colorless
• pH (1M Aqueous): 5.5 - 7.5
• Conductivity (1M Aqueous): ≤ 35 uS / cm
• Specific Rotation: 65.5° to 67.5°
• Water: ≤ 0.2%
• A260 (1M Aqueous): ≤ 0.09
• A280 (1M Aqueous): ≤ 0.07
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.

T4 DNA Ligase

Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.  This enzyme joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
 

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Intact Genomics T4 DNA Ligase displays up to 3-5X higher ligation efficiency than the nearest competitor.

Product Source
E. coli strain expressing a recombinant clone

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Contents & Storage

1. T4 DNA Ligase
2. 10x T4 DNA Ligase Reaction Buffer (w/o ATP)
3. 10 mM ATP

Store all contents at -20 °C.

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C

10x T4 DNA Ligase Reaction Buffer (w/o ATP) 
500 mM Tris-HCl, 100 mM MgCl, 100  mM DTT, pH 7.5 @ 25 °C

Note
10x T4 DNA ligase buffer does not contain ATP. You need to add ATP separately.

Unit Definition 
One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P from pyrophosphate into Norit-absorbance material in 20 minutes under standard assay conditions.

Protocol

1. Set up reaction buffer in a microcentrifuge tube on ice. Use a molar ratio of 1:3 vector to insert DNA.

1. Gently mix the reaction and centrifuge briefly.
2. For cohesive ends, incubate 16 °C for overnight or at room temperature for 30 min.
3. For blunt ends, incubate 16 °C for overnight or at room temperature for 2 hrs.
4. Heat inactivate at 70 °C for 15 min.
5. Cool on ice and transform 2 µl of the reaction into 50 µl competent cells.

ig-Fusion Cloning Kit 
Intact Genomics propriety ig-Fusion cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high-fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency.

Benefits

• Clone any insert at any site within any vector
• Restriction enzyme and phosphatase free system
• Joining multiple large fragments at once
• Precise insertion at a desired orientation
• Rapid and high efficiency with > 95% positive clones

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Contents & Storage

• 5x ig-Fusion enzyme premix: -20 °C
• 2x PCR premix: -20 °C
• High efficiency competent cells: -80 °C
• Recovery medium:4 °C or -20 °C

Protocol
1. Linearize the vector by restriction enzyme digestion or inverse PCR and purify the product with spin column.
2. Design PCR primers for the gene of interest with 15 to 20 bp at 5'-extensions that are complementary to the ends of the linearized vector.
3. Amplify the gene of interest with Intact Genomics 2x PCR premix or any other high-fidelity DNA polymerase. Run the PCR product on an agarose gel to determine the integrity of the PCR product.
4. Purify the PCR product with spin column.
5. Set up the ig-Fusion cloning reaction as follows: Insert and vector molar ratio 3:1 produce the highest number of colonies.  

6.  Mix the reaction mixture thoroughly.
7.  Incubate the reaction mixture at 50 °C for 10-30 min, then place on ice. Number of colonies depend on the incubation time, insert size and number of inserts need to clone.
8.  Use 2.0 µl of the reaction mixture and transform into high efficiency ig 10B chemical or electroporation competent cells (included). To get the maximum number of colonies, we recommend to use ig 10B electrocompetent cells (Cat # 1212).

MagBio HighPrep™ Plant RNA Plus Kit

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MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
• Tube volume: 3ml
• Dialysis volume: 0.1-3ml
• Min. sample size for extraction: 20µg
• Max. gel slice: 2cm x 1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 0.1-3ml
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
• Tube volume: 800µl
• Dialysis volume: 50-800µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 1cm x 0.5cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification.

MEGA Flex Tube Kit

MEGA Flex Tubes enable user friendly dialysis or buffer exchange of protein molecules with rapid, secure and simple loading along with high performance yield recovery.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K, 3.5K, 6-8K or 12-14K MWCO
• Tube volume: 10ml, 15ml or 20ml
• Dialysis volume: 3-20ml
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins

APPLICATIONS

• Dialysis or buffer exchange with volumes between 3-20ml
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification