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PCR DNA Clean-up Kit

The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.

Specifications
 
Sample SizeUp to 50 uL of PCR product
Up to 50 mg of agarose slice
Format96-Well Plates
OperationCentrifuge/Vacuum manifold
Binding Capacity10 uG per well
DNA Size50 bp - 10 kb
Recovery80-90% for Gel Extraction
90-95% for PCR Clean-Up
Operation Time30 min for PCR Clean-Up
40 min for Gel Extraction
 

Item#:
ASPCRCLEANKITB

PCR DNA Clean-up Kit

The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.

Specifications
 
Sample SizeUp to 50 uL of PCR product
Up to 50 mg of agarose slice
Format96-Well Plates
OperationCentrifuge/Vacuum manifold
Binding Capacity10 uG per well
DNA Size50 bp - 10 kb
Recovery80-90% for Gel Extraction
90-95% for PCR Clean-Up
Operation Time30 min for PCR Clean-Up
40 min for Gel Extraction
 

Item#:
ASPCRCLEANKITA

MagBio HighPrep™ Insect DNA Kit


Magnetic beads based kit designed to extract high-quality genomic DNA from insects, and arthropods (from one leg or wing to the whole body).


Applications

Genomic DNA isolation from insects for:

  •     PCR, Real-time PCR
  •     Cloning, genotyping
  •     Sequencing
  •     Insecticide resistance studies

Benefits

  •     Efficient isolation of PCR-quality DNA from insects, and arthropods
  •     No organic solvents
  •     Allows field epidemiologic research
  •     Magnetic beads based chemistry adaptable to automation

The HighPrep™ Insect DNA Kit is a high quality paramagnetic bead-based genomic DNA extraction kit used to isolate gDNA from a variety of insect-derived materials (wings, legs, whole body), and from different insect species, such as mosquitoes, flies, ants, and others. The HighPrep™ Insect DNA Kit is based on a fast and simple procedure which can either be performed manually or automated - up to 96 samples of tissues including legs and wings, can be processed in less than an hour.
Item#:
ASHIHPRPINSECTDK
  • RNase & DNase Free Water A new process of purifying large quantity of DNase and RNase free water enables us to offer Molecular Grade Water at a price that any laboratory can afford. 

  • No toxic agents such as DEPC are used in making the Molecular Grade Water; therefore, there is no chance of DEPC interfering with enzymatic reactions. 

  • The Molecular Grade Water is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase and RNase contamination. 

  • Molecular Grade Water can be used for making reagents and rinsing glass/plastic wares for DNA and RNA research. 

  • Each lot is quality tested for the absence of RNase and DNase contamination. Test reports are attached with each container as an added assurance.
Item#:
ASMOLWATER2

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® 10:1 Mixing Component Pack
 

Single-use components for 10:1 mixing ratio for VitroINK® Mixing Kit. DISPENSER NOT INCLUDED

The VitroINK® 10:1 Mixing Component Kit includes a 1 mL syringe, connector and mixing head to replace the one-time-use components in the full VitroINK® Mixing Kit – Complete Pack (Item # IMK00-1) for 10:1 VitroINK® and cells mixing.

This package is sterilized and disposable for one-time use.

 

Kit Contents and Specifications

  • Disposable mixing kit components for VitroINK® kit:
    • (1) 1 mL syringe
    • (1) connector and tubing
    • (1) mixing head
  • Sterile
  • Use for 10:1 mixing ratio
  • Ships room temperature. Store at room temperature.

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

  

Item#:
ASVITROINKM10TO1
Discontinued, Contact us for more options!


These items have been discontinued by the manufacturer.

See our MagBio HighPrep Total RNA Plus Kits or contact us for more options.


PrecisionPak Animal Tissue RNA Kit

 

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.

 

The PrecisionPak Animal Tissue RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology and additional reagents such as Proteinase K and DNase ensure very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

 

Tough Animal Tissue RNA Kits available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

 

Soft Animal Tissue RNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

Item#:
ASPPATRNAKIT
Discontinued, Contact us for more options!

This product has been discontinued.

Please visit the New PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye for more options!

 

 

PR1MA™ qMAX™ Gold

  • Inert yellow dye helps reduce pipetting errors
  • Room temperature stable for up to 30 days
  • Compatible with fast cycling protocols
  • Highly sensitive for low copy number templates
  • Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

MagBio HighPrep RNA Elite Clean-up System


Magnetic beads based reagent for manual and automated clean-up or concentration of RNA (including miRNA, siRNA, aRNA) and single-stranded cDNA after enzymatic reactions or prior sequencing.


Applications

RNA and ss cDNA clean-up or concentration for or after:

  •     cDNA synthesis
  •     RT-PCR
  •     Sequencing
  •     In vitro transcription
  •     RNA probe synthesis
  •     miRNA and siRNA preparation

Benefits

  •     Rapid and reliable clean-up of ss cDNA and RNA, including miRNA, siRNA, and aRNA
  •     Efficient purification of both large and small RNAs, and cDNA
  •     Complete removal of salts, unincorporated primers, and nucleotides
  •     No centrifugation step, no filtration step
  •     Adaptable to your current liquid handling workstation

The HighPrep™ RNA Elite Clean-up System utilizes MagBio’s solid-phase paramagnetic bead-based technology for high-throughput purification of RNA or cDNA for in vitro applications such as transcription, antisense RNA (aRNA) amplification as well as for RNA and cDNA probe synthesis. This protocol enables recovery even of micro RNA (miRNA), small RNA and total RNA from enzymatic reactions, concentrating of miRNA and total RNA from a diluted sample. The HighPrep™ RNA Elite Clean-up System can be used for manual RNA clean-up as well as on automated liquid handling workstations.
Item#:
ASHIHPRPRNAECUS
1mL, 1 vial of 1000ul
This item has been discontinued and replaced by item 

Affordable price & shipped at room temperature.

 

A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.

  • 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
  • This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
  • The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
  • The elongation velocity is 0.9~1.2kb/min (70~75°C).
  • It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.

 

Features:

  • Convenient: Just add primers and template DNA
  • High yields of PCR products with minimal optimization.
  • High efficiency: saves your time by simplifying the process
  • Reproducible: lower contamination risk and pipetting error.

    Applications:

  • High-throughput PCR.
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning

    Contents:
    2X Taq Mix 1ml

    Store at -20°C
    For research use only

Item#:
ECO-TAQ1
Your Price:
90.00
Each
This item is discontinued


Sucrose

  • Appearance (Color): White
  • Appearance (Form): Crystalline Powder
  • Purity: ≥ 99.0%
  • Identity (FTIR): Conforms to Structure
  • Solubility (1M Aqueous): Clear and Colorless
  • pH (1M Aqueous): 5.5 - 7.5
  • Conductivity (1M Aqueous): ≤ 35 uS / cm
  • Specific Rotation: 65.5° to 67.5°
  • Water: ≤ 0.2%
  • A260 (1M Aqueous): ≤ 0.09
  • A280 (1M Aqueous): ≤ 0.07
  • RNase: None Detected
  • DNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.

 

Item#:
ASUSROSE1

MagBio cfKapture™ Kit


Magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum.


Applications

cfDNA (ccfDNA) extraction for:
  •     Liquid biopsy research
  •     NIPT genomic research
  •     Cancer genomic research
  •     Transplant monitoring

Benefits

  •     Efficient Isolation of nucleic acids, with high input and low elution volumes
  •     No carrier RNA
  •     Low sample volume processing
  •     Efficient recovery of small sized fragmented DNA
  •     No organic extraction or ethanol precipitation

 
The cfKapture™ kit is a magnetic bead-based DNA extraction kit designed to isolate circulatingcell freeDNA (cfDNA) from human plasma and serum. The kit has a quick, simple, and automation friendly protocol that allows users to quickly extract high quality cfDNA that is ready for use in downstream applications such as qPCR, NGS, and bisulfite sequencing.

 
Item#:
ASCFKAPTKIT
Discontinued, Contact us for more options!


These items have been discontinued by the manufacturer.

See our MagBio HighPrep Blood & Tissue DNA Kits or contact us for more options.

PrecisionPak Animal Tissue DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality DNA.

 

The PrecisionPak Animal Tissue DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensures very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

 

Tough Animal Tissue DNA Kit available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larsynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

 

Soft Animal Tissue DNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

Item#:
ASPPATDNAKIT
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