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PR1MA™ Permeable Cell Culture Inserts

PR1MA™ Permeable Cell Culture Inserts are designed to support advanced cell culture applications by mimicking a variety of in vivo conditions. With multiple membrane materials and pore size options, these inserts provide flexibility for a wide range of applications, including angiogenesis, migration, chemotaxis experiments, cell interaction, and drug transport studies.

Insert design ensures proper centering within wells, allowing easy pipette access to the lower chamber while preventing unwanted wicking between the insert and side walls. Suspended above tissue culture-treated wells, these inserts enable efficient co-culture setups, allowing cells to grow both on the membrane and at the bottom of the well.

*Non-treated plates are sold separately.

For optimized performance, PET membranes offer excellent clarity for imaging and microscopy, while PC membranes provide superior cell attachment. This combination of versatility, ease of use, and reliable performance makes PR1MA™ inserts a valuable tool for cell culture workflows.

Main Attributes

• Designed to mimic in vivo conditions with 2 membrane materials and 4 pore sizes
• Centered insert design allows pipette access and prevents wicking
• Supports co-culture systems with growth on both membrane and well bottom
• Compatible with tissue culture-treated wells (non-treated plates sold separately)
• Clear PET membranes (0.1 uM and 0.4 uM) for enhanced cell visualization
• PC membranes for optimal cell attachment 

Pore Size Applications

• 0.1 uM and 0.4 uM: Ideal for cell-cell interaction and drug transport studies
• 3.0 uM and 8.0 uM: Ideal for angiogenesis, migration, and chemotaxis experiments

NEST Cell Culture Inserts

• Innovative hanging design facilitates pipetting.
• Supplied in multiple well plates: 6-well, 12-well, 24well.
• PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
• Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
• Transparent PET membrane is easy to observe.
• The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
• Compatible with most solvents used for fixing and dyeing.

Attention

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
• During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
• Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Material

Multiple well plate or dish is made from PS.

Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.