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Supplies
High-Quality Laboratory Supplies for Reliable Research
MIDSCI™ understands that the right lab supplies are the foundation of reliable research across the life sciences. When your lab relies on frequent replenishment of essential scientific supplies, we deliver the labware you need with the speed and personalized service you don’t see with big-box distributors.
From glassware and plasticware to PPE, pipette tips, test tubes, and reagent reservoirs, we offer products designed for accuracy, durability, and compliance. Whether you work in life sciences, microbiology, or pharmaceutical development, our lab essentials help you maintain efficiency without compromising quality.
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ig 5-Alpha
Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.
- Up to 2x higher efficiency than any competitor
- Exact same Genotype as NEB's 5-Alpha Cells
Number of Colonies per Transformation Test*
Specifications
Competent cell type: Chemically competent
Derivative of: 5-alpha
Species: E. coli
Format: Tubes
Transformation efficiency: 1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes
Shipping condition: Dry ice
Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching.
Reagents Needed for One Reaction
ig 5-alpha chemically competent cells: 50 µl
DNA (or pUC19 Control, 10 pg/µl): 1 µl
Recovery medium: 1 ml
Contents & Storage
ig 5-alpha competent cells: -80 °C
pUC19 control DNA: -20 °C
Recovery medium: 4 °C
Genotype
80 (lacZ)M15 fhuA2 (argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17
General Guidelines
Follow these guidelines when using ig 5-alpha chemically competent cells.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.
Protocol
- Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
- Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
- When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping. 4) Incubate the cells with DNA on ice for 30 minutes.
- After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
- Transfer the tubes to ice for 2 minutes.
- Add 950 µl of Recovery Medium or any other medium of choice to each tube.
- Incubate tubes at 37 °C for 1 hour at 210 rpm.
- Spread 50 μl to 200 μl from each transformation on Pre-warmed selection plates.
- Incubate the plates overnight at 37 °C.
- Provides exceptional seal to safeguard samples during transport or storage
- Eliminates well to well contamination and cross-over in PCR applications when used with Axygen's compression mat
- Polypropylene film is wide temperature range compatible, -40° to 104°C
- PCR-TS is designed for ELISA/EIA applications
- CR-TS-900 is twice as thick (as PCR-TS) and is suitable for water-bath and other difficult PCR applications
Beckman Style Robotic Pipette Tips
Our robotic tips fit Beckman FX/NX®, Multimek AP96® and Biomek 3000®
- Case dimensions are
- 20 uL: 420(L) x 345(W) x 280(H) mm
- 50 uL & 250 uL: 480(L) x 345(W) x 280(H) mm
- Gross weight is
- 20 uL: 9.8 kg
- 50 uL & 250 uL: 10.1 kg
- 96 tips/hanging tip rack format
- Polypropylene
- Available filtered and non-filtered
- Available presterilized and nonsterile
- Certified RNase and DNase Free
- Nonpyrogenic
- Endotoxin-Safe meets USP/FDA Standards
- Comply with USP 3127 (Heavy Metal Contamination) standard
- Tips are liquid level sensing tips
Cytiva 3MM Chr paper is the world's most widely used blotting paper.
This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.
GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.
GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.
17 Chr
A thick (0.92 mm) and highly absorbent paper.
31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.
Features and Benefits
- Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
- Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
- Cytiva 3MM Chr is considered the industry standard for blotting procedures
- Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations
3MW is used widely in nucleic acid and protein research for blotting, wicking, spacers, and gel drying due to its purity, quality and consistent results. Uniform capillary action provides even wicking and enables gel transfers to be lifted RELIABLY from glass supports.
Cytiva 3MM Chr paper is the world's most widely used blotting paper.
This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.
GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.
GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.
17 Chr
A thick (0.92 mm) and highly absorbent paper.
31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.
Features and Benefits
- Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
- Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
- Cytiva 3MM Chr is considered the industry standard for blotting procedures
- Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations
This item has been discontinued.
Silicone Sealing Mat for 96 plates
- Made of research-grade silicone
- Scored wells for sample access
- Perforations re-seal after access
- Resists coring and tearing
- Eliminates cross-talk between wells
- Also works as a compression mat
- Suitable for standard PCR thermocycling
- Mats can be reused
- For use with 96-well PCR plates
- Printed alpha numeric grid for easy reference
- Certified to be free of RNase, DNase, DNA, PCR inhibitors, and Pyrogen contamination.
Works as a plate seal (to eliminate cross contamination) and compression mat all-in-one!
Our compression molded 96-round plug, silicone sealing mats for PCR plates minimize sample evaporation in a wide range of temperatures. These mats are chemically resistant and reusable; containing no adhesives or extractables to contaminate samples. Made of research-grade silicone, they can be autoclaved, and following a 10% bleach wash and ethanol rinse protocol may be reused.
Wells are scored (cut 80% of the way through, but not all of the way) to allow for sample access via pipettor, syringe, or probe. These scored perforations will seal themselves, after penetration, ensuring an airtight closure to the well.
- Research Grade
- Beveled
- Fits Pipetman
- AlphaPette
- All major brands with volume up to 200µl
AxyMat Sealing Mats
- Multi-use silicone sealing mats
- Natural color
- Autoclavable and washable
- Pierceable and self-sealing
AxyMat Sealing Mats
These items have been discontinued.
Have questions or need more information? Feel free to contact us!
General Purpose 4-Ply Mask
Designed for general safety and non-critical exposure, such as, receptionist, janitorial staff, non-invasive procedures, non-fluid contact, etc.
FDA Product Code: QKR
FDA Definition: Face mask is intended to be worn by general public or healthcare personnel. The mask covers the users face and mouth and may or may not meet fluid barrier or filtration efficiency levels. The device is subject to ED if labeling conditions outlined in the IIE guidance (https://www.fda.gov/regulatory-information/search-fda-guidance-documents/enforcement-policy-face-masks-and-respirators-during-coronavirus-disease-covid-19-public-health) are met, for the duration of the COVID-19 public health emergency.
We are working daily with our supply partners and logistics providers to address shortages & delays as they occur. Considering the current conditions, we anticipate supply disruptions for all PPE products. Moreover, China is retaining significantly more PPE products for use within their country that were originally slated for export and policies are changing rapidly. There has also been changes to how the products are being packaged and shipped. Product that meets CDC certification may or may not be marked as “not for medical use.” This combination of factors may lead to delays and changes in manufacturing, labeling, packaging, and/or shipments of these select products that MIDSCI sources from China.
The evaluation for allocation, as goods are secured, will include multiple factors, most notably historical demand run rates. We are diligently working to find new sources to supplement our current suppliers and enhance our ability to fill orders. As this develops, our team will provide details on how we can improve the current situation. If you have any questions, please reach out to us throughout this process, so we can answer any of your questions.
MIDSCI remains committed to balanced distribution of all protective products that both supports the public health and governmental response to the Coronavirus and ensures product availability to our existing industrial and healthcare customers whose operations rely on our products.
In response to the outbreak of Coronavirus (COVID-19)
All sales are final, non-cancellable, and non-returnable
These items have been discontinued.
Silicone Sealing Mat for 34, 48, 96, and 384-well
- Made of research-grade silicone
- Perforated wells for easy sample access
- Re-sealable perforations
- Resists coring and tearing
- Eliminates cross-talk between wells
- Suitable for high-temperature storage
- Mats can be reused
- For use with assay and storage plates
- Printed alpha numeric grid for easy reference
- Used for sample and compound storage.
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