RV-Pette PRO Dispenser Tip for Repeat Volume Pipettors

Dispenser Syringe Tips for use on Diamond Rv-Pette PRO™ Repeat Volume PipettorS. Precision manufacturing under strict quality controls ensures that RV-Pette PRO™ Dispenser Syringe Tips deliver excellent results when used with Diamond RV-Pette PRO™ Repeat Volume Pipettors and most popular brands of repeat volume pipettors. RV-Pette PRO™ tips are available in 12 sizes for dispensing volumes from 1 uL to 5500 uL and are available sterile or non-sterile.

Features and Benefits

Variety - Wide range of sizes match all sizes offered by other manufacturers and eliminates the need to change protocols or practices

Safety - Tips use the direct displacement method and are the safe, secure choice for handling a wide range of liquids including those that are viscous, infectious, and volatile

Reliability - Each tip is tested during the automated manufacturing process

Confidence - Sterile tips are individually wrapped and certified to be RNase, DNase, ATP, and pyrogen free for even the most critical applications

ig BL21 (DE3)
Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.

Specifications
Competent cell type:                Chemically competent
Derivative of:                            BL21(DE3)
Species:                                   E. coli
Format:                                    Tubes
Transformation efficiency:         1.0 x 109 cfu/µg pUC19 DNA
Blue/white screening:               Yes
Shipping condition:                   Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
BL21(DE3) Chemically Competent Cells:             50 µl
DNA (or pUC19 Control, 10 pg/µl):                         1 µl
Recovery Medium:                                                 1 ml

Contents & Storage
BL21(DE3) Competent Cells:                -80 °C
pUC19 Control DNA:                            -20 °C
Recovery Medium:                                  4 °C

Genomic Features
BL21(DE3) chemically competent cells have the following features:

• Widely used host background
• T7 Expression Strain
• Deficient in both lon (1) and ompT proteases
• Resistant to phage T1 (fhuA2)
• B Strain

Genotype
F-ompT hsdS(rB- mB-) gal dcm (DE3)

General Guidelines
Follow these guidelines when using BL21(DE3) chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Transformation Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl)
• DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
• Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on pre-warmed selection plates.
• Incubate the plates overnight at 37 °C. 

ig 5-Alpha
Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.

Number of Colonies per Transformation Test*

• Up to 2x higher efficiency than any competitor
• Exact same Genotype as NEB's 5-Alpha Cells

Specifications
Competent cell type:        Chemically competent
Derivative of:                    5-alpha
Species:                            E. coli
Format:                             Tubes
Transformation efficiency:      1 x 109 cfu/µg pUC19 DNA
Blue/white screening:           Yes
Shipping condition:               Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
ig 5-alpha chemically competent cells:  50 µl
DNA (or pUC19 Control, 10 pg/µl):           1 µl
Recovery medium:                                   1 ml

Contents & Storage
ig 5-alpha competent cells:   -80 °C
pUC19 control DNA:                 -20 °C
Recovery medium:                       4 °C

Genotype
80 (lacZ)M15 fhuA2 (argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17

General Guidelines
Follow these guidelines when using ig 5-alpha chemically competent cells.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping. 4) Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on Pre-warmed selection plates.
• Incubate the plates overnight at 37 °C.

ig 10B
Intact Genomics ig 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.

• Most efficient chemically competent cells in the industry (up to 10X more efficient!)
• May easily be used in place of NEB 10-Beta, TOP10 from Invitrogen or other common brands
• Significantly more affordable

Specifications
Competent cell type:                Chemically competent
Derivative of:                            DH10B
Species:                                   E. coli
Format:                                    Tubes
Transformation efficiency:         â¥1.0 x 1010 cfu/µg pUC19 DNA
Blue/white screening:               Yes
Shipping condition:                   Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
ig 10B chemically competent cells:     50 µl
DNA (or pUC19 Control, 10 pg/µl):          1 µl
Recovery medium:                                  1 ml

Contents & Storage

1. ig 10B competent cells: -80 °C
2. pUC19 control DNA: -20 °C
3. Recovery medium: 4 °C

Genomic Features
ig 10B (DH10B derivative) chemically competent cells have the following features:

• 80lacZM15 marker provides α-complementation of the β-galactosidase gene with blue/white screening
• mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine

Genotype
F â mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara, leu)7697 galU galK rpsL (StrR) nupG -

General Guidelines
Follow these guidelines when using ig 10B chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 µl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
• Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 µl to 200 µl from each transformation on Pre-warmed selection plates.
• Incubate the plates overnight at 37 °C.

Beckman Style Robotic Pipette Tips

Our robotic tips fit Beckman FX/NX®, Multimek AP96® and Biomek 3000®

• Case dimensions are
• 20 uL: 420(L) x 345(W) x 280(H) mm
• 50 uL & 250 uL: 480(L) x 345(W) x 280(H) mm
• Gross weight is
• 20 uL: 9.8 kg
• 50 uL & 250 uL: 10.1 kg
• 96 tips/hanging tip rack format
• Polypropylene
• Available filtered and non-filtered
• Available presterilized and nonsterile
• Certified RNase and DNase Free
• Nonpyrogenic
• Endotoxin-Safe meets USP/FDA Standards
• Comply with USP 3127 (Heavy Metal Contamination) standard
• Tips are liquid level sensing tips

Cytiva 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.

GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.

GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.

17 Chr
A thick (0.92 mm) and highly absorbent paper.

31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.

Features and Benefits

• Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
• Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
• Cytiva 3MM Chr is considered the industry standard for blotting procedures
• Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations

3MW is used widely in nucleic acid and protein research for blotting, wicking, spacers, and gel drying due to its purity, quality and consistent results. Uniform capillary action provides even wicking and enables gel transfers to be lifted RELIABLY from glass supports.

Cytiva 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for blotting in electrophoresis for lifting of sequencing gels.

GB004
A thick gel blotting paper (1.0 mm), used for wicking purposes only. Provides higher absorbency and more consistent wicking than paper towels. Recommended for applications where fewer layers of gel blotting paper must still ensure a high capacity. Fewer layers of blotting paper reduce the risk of trapping air bubbles. Recommended for capillary blotting of nucleic acids.

GB005
An extra-thick (1.2 mm ), highly absorbent paper recommended for applications where fewer layers of blotting paper must siill ensure a high capacity. Recommended for semi-dry blotting of proteins.

17 Chr
A thick (0.92 mm) and highly absorbent paper.

31 ET
An extremely fast and thick paper (0.5 mm) with a fairly soft surface.

Features and Benefits

• Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
• Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
• Cytiva 3MM Chr is considered the industry standard for blotting procedures
• Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations

This item has been discontinued.

Check out our other Sealing Mats for more options!

• Made of research-grade silicone
• Scored wells for sample access
• Perforations re-seal after access
• Resists coring and tearing
• Eliminates cross-talk between wells
• Also works as a compression mat
• Suitable for standard PCR thermocycling
• Mats can be reused
• For use with 96-well PCR plates
• Printed alpha numeric grid for easy reference
• Certified to be free of RNase, DNase, DNA, PCR inhibitors, and Pyrogen contamination.

Works as a plate seal (to eliminate cross contamination) and compression mat all-in-one!

Our compression molded 96-round plug, silicone sealing mats for PCR plates minimize sample evaporation in a wide range of temperatures. These mats are chemically resistant and reusable; containing no adhesives or extractables to contaminate samples.  Made of research-grade silicone, they can be autoclaved, and following a 10% bleach wash and ethanol rinse protocol may be reused.

Wells are scored (cut 80% of the way through, but not all of the way) to allow for sample access via pipettor, syringe, or probe. These scored perforations will seal themselves, after penetration, ensuring an airtight closure to the well.
  

AxyMat Sealing Mats

• Multi-use silicone sealing mats
• Natural color
• Autoclavable and washable
• Pierceable and self-sealing