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PetriSeal™ Sealing Film

Item #: ASSEAL4 

Specifications

Length 2,160 In. | 54864 mm
Width 0.50 In. | 12.7 mm
U of M Roll

 

Bullseye

This brand is being discontinued, please contact us for other options.

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options! 


Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits

Bullseye offers a product series specifically developed for the amplification of GC-rich DNA sequences. The Bullseye HS DNA Polymerase combined with GC Buffer I and GC Buffer II promote excellent amplification results with targets of varying degrees of GC content. Hot Start DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 10-15 minutes heat activation step, releasing the active Hot Start DNA Polymerase into the reaction. The GC Buffers in combination with Hot Start DNA Polymerase and the heat activation step result in a high success rate in amplification of DNA targets with high GC content.

Key Features

  • Amplification of multiple DNA targets with high GC content
  • High specificity, sensitivity and product yield
  • Diminished formation of non-specific product
  • Detection of low copy number targets

Kit Components

  • HS DNA Polymerase in Storage Buffer
  • 5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
  • mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20,
  • 0.5% NP40, 50% glycerol.
  • 4x GC Buffer I
  • Optimized buffer components, 6 mM MgCl
  • 4x GC Buffer II
  • Optimized buffer components, 6 mM MgCl
  • MgCl
  • 25 mM MgCl in PCR grade water
High Fidelity Polymerase
Item#:
ASACCREAG4
 
High Fidelity Hot Start M
Item#:
ASACCURISHIFIDHS
 
SKU Number Type Size Color Price Quantity Add to Cart
PSEAL-108B PetriSEAL 5 In. x 150 Ft. Transparent
33.3300
33.00
Each
PSEAL-108C PetriSEAL 0.5 In. x 108 Ft. Clear
33.3300
33.00
Each
PSEAL-108W PetriSEAL 0.5 In. x 108 Ft. White
33.3300
33.00
Each
PSEAL-108R PetriSEAL 0.5 In. x 108 Ft. Red
33.3300
33.00
Each
PSEAL-108Y PetriSEAL 0.5 In. x 108 Ft. Yellow
33.3300
33.00
Each
PetriSEAL
PetriSeal™ Sealing Film
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