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Item #: ASPOSSLIDES 
PCR DNA Clean-up Kit

PCR DNA Clean-up Kit

The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.

Specifications
 
Sample SizeUp to 50 uL of PCR product
Up to 50 mg of agarose slice
Format96-Well Plates
OperationCentrifuge/Vacuum manifold
Binding Capacity10 uG per well
DNA Size50 bp - 10 kb
Recovery80-90% for Gel Extraction
90-95% for PCR Clean-Up
Operation Time30 min for PCR Clean-Up
40 min for Gel Extraction
 

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Diamond White Microscope
Diamond Microscope Slides Positively Charged
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