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MIDI Flex Tube Kit
MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.
KIT CONTENTS
- Flex Tubes 2/10/30/50/ 100 pieces
- Supporting tray (for electro elution protocol) 1ea. (select kits)
- Floating rack (for dialysis protocol) 1ea. (select kits)
- Information and Protocol Manual 1ea.
SPECIFICATIONS
- Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
- Tube volume: 800µl
- Dialysis volume: 50-800µl
- Min. sample size for extraction: 0.5µg
- Max. gel slice: 1cm x 0.5cm
- Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
- Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:
kilo Daltons |
base pairs |
1K |
3 bp |
3.5K |
11bp |
6-8K |
18-24bp |
12-14K |
36-42bp |
25K |
76bp |
50K |
152bp |
APPLICATIONS
- Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis, such as antibodies and recombinant protein purification
- Removal of contaminating micro-molecules
- Tissue culture extraction purification
- Removal of salts, surfactants, solvents, and detergents
- Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
- pH and buffer adjustment of sample solutions, protein extraction or cell extraction
- High throughput dialysis
- Peptide dialysis, as small as 10 amino acids
- Virus-particles purification.
MEGA Flex Tube Kit
MEGA Flex Tubes enable user friendly dialysis or buffer exchange of protein molecules with rapid, secure and simple loading along with high performance yield recovery.
KIT CONTENTS
- Flex Tubes 2/10/30/50/ 100 pieces
- Floating rack (for dialysis protocol) 1ea. (select kits)
- Information and Protocol Manual 1ea.
SPECIFICATIONS
- Membrane cut-off: 1K, 3.5K, 6-8K or 12-14K MWCO
- Tube volume: 10ml, 15ml or 20ml
- Dialysis volume: 3-20ml
- Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
- Flex Tube MWCO are in kilo Daltons (K) for proteins
APPLICATIONS
- Dialysis or buffer exchange with volumes between 3-20ml
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis, such as antibodies and recombinant protein purification
- Removal of contaminating micro-molecules
- Tissue culture extraction purification
- Removal of salts, surfactants, solvents, and detergents
- Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
- pH and buffer adjustment of sample solutions, protein extraction or cell extraction
- High throughput dialysis
- Peptide dialysis, as small as 10 amino acids
- Virus-particles purification
MINI Flex Tube Kit
MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.
KIT CONTENTS
- Flex Tubes 2/10/30/50/ 100 pieces
- Supporting tray (for electro elution protocol) 1ea. (select kits)
- Floating rack (for dialysis protocol) 1ea. (select kits)
- Information and Protocol Manual 1ea.
SPECIFICATIONS
- Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
- Tube volume: 250µl
- Dialysis volume: 10-250µl
- Min. sample size for extraction: 0.5µg
- Max. gel slice: 0.4cm x 1.1cm
- Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
- Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:
kilo Daltons |
base pairs |
1K |
3 bp |
3.5K |
11bp |
6-8K |
18-24bp |
12-14K |
36-42bp |
25K |
76bp |
50K |
152bp |
APPLICATIONS
- Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis, such as antibodies and recombinant protein purification
- Removal of contaminating micro-molecules
- Tissue culture extraction purification
- Removal of salts, surfactants, solvents, and detergents
- Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
- pH and buffer adjustment of sample solutions, protein extraction or cell extraction
- High throughput dialysis
- Peptide dialysis, as small as 10 amino acids
- Virus-particles purification
These items have been discontinued by the manufacturer.
See our Extraction and Purification page or contact us for more options.
PrecisionPak Small Organism DNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high quality DNA.
The PrecisionPak Small Organism DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensure very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.
Tough Small Organism DNA Kit available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.
Soft Small Organism DNA Kits available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.
These items have been discontinued by the manufacturer.
See our Extraction and Purification page or contact us for more options.
PrecisionPak Small Organism RNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.
The PrecisionPak Small Organism RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and DNase ensures very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.
Tough Small Organism RNA Kits available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.
Soft Small Organism RNA Kit available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.
MagBio CTL™ Medium (Collect - Transport - Lyse)
- Sample collection, lysis, inactivation and stabilzation for - Viral, Fungal & Bacterial Samples (Swabs)
- DNA & RNA sample preparation for pathogen detection
- Flexibility in sample processing - Room Temperature Stability
- DNA = 45 Days | RNA = 8 Days
- COVID-19 Detection
- Significantly shortens sample preparation time in pathogen testing & detection workflows
- Cost savings - Extraction & extraction accessories not required
- Preserves and maintains the integrity of nucleic acids at room temperature:
- DNA = 45 Days | RNA = 8 Days
- Compatible with various extraction or purification workflows
- Samples will be lysed during transport - No need for extraction / lysis.
- Allows samples to be batched which can in turn improve the processing efficiency
, Beads
IBI MIDI I-Blue Plasmid Sample
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
MagBio HighPrep™ Plasmid DNA Plus Kit
Magnetic beads based kit designed to extract high-quality plasmid DNA (including Cosmids, Fosmids, and BACs) from bacterial cultures.
- PCR, Real-time PCR
- Cloning, genotyping
- Sequencing, NGS
- Transfection
- High quality plasmid DNA purification
- High yields of both high and low copy number plasmids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Plasmid DNA kit is a magnetic beads-based plasmid purification system for high throughput rapid isolation of both high and low copy number plasmid DNA from bacterial cultures as well as for isolation of BACs, PACs, Cosmids or Fosmids. The system can easily be automated on many liquid handling workstations, providing robustness and high-quality plasmid DNA for subsequent downstream applications such as restriction digestion, PCR, cloning and fluorescent DNA sequencing.
, Beads
The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.
Sample Types: |
Blood, cultured cells, body fluids, and other animal cells |
Sample Size: |
5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood |
Format: |
Spin columns |
Operation: |
Centrifuge/Vacuum |
Binding Capacity: |
Up to 60µg |
Time: |
30 minutes |
Applications: |
RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis |
Advantages
- Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
- Yield: Up to 5 µg of gDNA from fresh whole blood samples
- Format: genomic DNA spin column
- Operation Time: Within 20 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year
Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Kit Components
Component |
IB47280 |
IB47281 |
IB47282 |
GST Buffer |
3 mL |
30 mL |
75 mL |
GSB Buffer |
4 mL |
40 mL |
75 mL |
W1 Buffer |
2 mL |
45 mL |
130 mL |
Wash Buffer* |
1 mL |
25 mL |
50 mL |
Proteinase K** |
1 mg |
11 mg x 2 |
65 mg |
Elution Buffer |
1 mL |
30 mL |
75 mL |
GD Columns |
4 |
100 |
300 |
2 mL Collection Tubes |
8 |
200 |
600 |
*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.
**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.
- Sample: Gram (+) positive and Gram (-) negative bacterial cells
- Yield: Up to 30 µg of gDNA - (1 x 10 Escherichia coli: 25-30 µg, 1 x 10 Bacillus subtilis: 10-15 µg)
- Convenient: Includes Gram+ Buffer for preparing lysozyme solutions and to speed up sample preparation
- Format: Genomic DNA spin columns
- Operation Time: within 30 minutes
- Elution Volume: 50-200 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year
The gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.
Quality Control
The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.
Items |
IB47290 |
IB47291 |
IB47292 |
Gram+ Buffer* |
1.5 mL |
30 mL |
75 mL |
GT Buffer |
1.5 mL |
30 mL |
75 mL |
GB Buffer |
2 mL |
40 mL |
100 mL |
W1 Buffer |
2 mL |
45 mL |
130 mL |
Wash Buffer** |
1 mL |
25 mL |
50 mL |
Elution Buffer |
1 mL |
30 mL |
75 mL |
GD Columns |
4 |
100 |
300 |
2 mL Collection Tubes |
8 |
200 |
600 |
*Add lysozyme to Gram+ Buffer immediately prior to use. Once lysozyme is mixed with Gram+ Buffer, the solution can be stored for 2 weeks at 4°C.
**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.
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