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Reagents
Trusted, High‑Purity Reagents for Reliable Life Science Research
MIDSCI™ offers a wide selection of high‑quality laboratory reagents engineered for accuracy, reproducibility, and dependable performance. Whether you're running PCR, qPCR, ELISA, electrophoresis, or nucleic acid purification, our reagents are designed to help you collect consistent, high‑quality data. From enzymes and buffers to DNA ladders and biochemical components, every product meets strict purity and ISO‑aligned quality standards, giving your research the dependable foundation it deserves.
Our reagents support a wide range of molecular biology, microbiology, and biochemical applications. Designed to minimize impurities and enhance performance in sensitive assays, MIDSCI™ reagents help ensure clean results, reliable amplification, clear electrophoresis bands, and low‑background immunoassays. Whether you're working in an academic, clinical, or industrial lab, you’ll find the right tools to support your workflows.
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Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits
Bullseye offers a product series specifically developed for the amplification of GC-rich DNA sequences. The Bullseye HS DNA Polymerase combined with GC Buffer I and GC Buffer II promote excellent amplification results with targets of varying degrees of GC content. Hot Start DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 10-15 minutes heat activation step, releasing the active Hot Start DNA Polymerase into the reaction. The GC Buffers in combination with Hot Start DNA Polymerase and the heat activation step result in a high success rate in amplification of DNA targets with high GC content.Key Features
- Amplification of multiple DNA targets with high GC content
- High specificity, sensitivity and product yield
- Diminished formation of non-specific product
- Detection of low copy number targets
Kit Components
- HS DNA Polymerase in Storage Buffer
- 5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
- mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20,
- 0.5% NP40, 50% glycerol.
- 4x GC Buffer I
- Optimized buffer components, 6 mM MgCl
- 4x GC Buffer II
- Optimized buffer components, 6 mM MgCl
- MgCl
- 25 mM MgCl in PCR grade water
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Bullseye PREMIUM HS-Taq DNA Polymerase Master Mix with HS Buffer I
| Cat.No. | Size Reactions | HS Taq, 2X Mix (Buffer I) | Final MgCl2 Conc. |
| BE230301 | 100 | 2X HS Buffer I Mix | 1.5 mM |
| BE230303 | 500 | 2X HS Buffer I Mix | 1.5 mM |
| BE230304 | 1,000 | 2X HS Buffer I Mix | 1.5 mM |
| BE230306 | 2,500 | 2X HS Buffer I Mix | 1.5 mM |
Store at -20°C. For in-vitro laboratory use only
Bullseye HS Taq Polymerase, 2X Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Bullseye HS Taq Polymerase Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer I. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.
Bullseye HS Taq Polymerase Mix is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.
Bullseye" HS Taq Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Composition of HS Taq Pol, 2x Mix
Tris-HCl pH 8.5, (NH4)2S04, 3.0mM MgCl2, 0.2% Tween 20Ã, 0.4 mM dNTPs, 0.2 units/uL HS Hot Start DNA Polymerase Stabilizer
Suggested Protocol using HS Taq Pol, 2x Mix
This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.
-
Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
-
The table below shows the reaction set up for a final volume of 50 mL.
-
Important: Mix the solutions completely before use to avoid localized concentrations of salts.
1. Set up each reaction as follows:
| Component | Vol./Reaction | Final Conc. |
| HS Hot Start Master Mix w/ HS Bufffer I | 25 uL | 1X |
| Primer A | Variable | 0.11.0 uM |
| Primer B | Variable | 0.11.0 uM |
| Distilled Water | Variable | - - - - |
| Template DNA | Variable | Variable |
| TOTAL volume | 50 uL | - - - - |
2. Mix gently by pipetting the solution up and down a few times.
3. Program the thermal cycler according to the manufacturer's instructions.
4. Each program must start with an initial heat activation step at 95°C for 15 minutes.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
A typical thermal cycling program is shown below:
95°C for 15 min. Activate HS Hot Start Polymerase
30-40 cycles:
95°C 30 sec Denature template
45-65°C 30 sec Anneal primer
72°C 1-5 min Elongation
72°C for 5 min Elongation
5. Place the tubes in the thermal cycler and start the reaction.
This brand is being discontinued and will only be available while supplies last.
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Bullseye PREMIUM Std Taq
- Excellent all-purpose amplification enzyme
- Thermostable recombinant DNA polymerase from Thermus aquaticus
- Exhibits very high activity in primer extension
- Has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity
- Does not have 3' to 5' exonuclease activity-no proofreading ability
- Leaves an A-overhang, which makes the enzyme ideal for TA cloning
- Includes MgCl² in buffer
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Bullseye PREMIUM Taq DNA Polymerases
Store at -20°C. For in-vitro laboratory use onlyBullseye Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
Bullseye Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A¢ overhang, which makes the enzyme ideal for TA cloning.
The 10X Reaction Buffer provided does not contain Mg+2. 25 mM MgCl2 is supplied separately.
10X Mg++ Free Standard Buffer
100 mM Tris-HCl pH 8.3, 500 mM KCl.
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage and Dilution Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5%NP40, 50% glycerol.
Quality Control
Each lot of Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.
Suggested Protocol using Taq DNA Polymerase
This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.
n In some applications, MgCl2 is needed for the best results. For this reason, 25 mM MgCl2 is included with the kit.
1. Thaw 10X Mg2+ Free Standard Buffer, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
Table 1. Reaction components (master mix & template DNA)
| Component | Vol./reaction | Final Conc. |
| 10X Mg2+ Free Buffer | 5 uL | 1X |
| MgCl2, 25 mM | 1- 9 uL | 0.5 4.5 mM |
| dNTP mix (12.5 mM of each) | 0.8 uL | 0.2 mM of each dNTP |
| Primer A | Variable | 0.11.0 uM |
| Primer B | Variable | 0.11.0 uM |
| Taq DNA Polymerase | Variable | 1-5 units |
| Template DNA | Variable | Variable |
| Distilled Water | Variable | - - - - |
| TOTAL volume | 50 uL | - - - - |
Table 2. MgCl2 concentration in a 50 mL reaction
| Final MgCl2 conc. in reaction (mM) | 0.5 | 1 | 1.5 | 2 | 2.5 | 3 | 3.5 | 4 | 4.5 |
| Vol. of 25 mM MgCl2 per rxn (uL) | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a certain MgCl2 concentration is required.
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.
4. Add template DNA to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturers instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
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2x Master Mix Kit (1.5 mM MgCl2)
Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)
Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Composition of 2x Taq Master Mix
150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2%
Tween 20Ò
4 mM dNTPs
2 units/µL AS ONE Taq polymerase
Inert Red Dye & Stabilizer (BE180303 only)
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Bullseye PREMIUM R-Taq DNA Polymerase
R-Taq DNA Polymerase 5 units/µL
| Item # | Units | 10X Ammonium Buffer (MgCl2 15mM) | MgCl2 25 mM |
| BE200303 | 500 | 1.5 mL | 1.5 mL |
| BE200304 | 1,000 | 2x 1.5 mL | 2x 1.5 mL |
| BE200306 | 2,500 | 4x 1.5 mL | 4x 1.5 mL |
Store at -20°C. For in-vitro laboratory use only
Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.
Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.
- High performance thermostable DNA polymerase
- Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
- Leaves an A-overhang
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.
| Component | Vol./reaction | Final Conc. |
| 10X Ammonium Buffer | 5 uL | 1X |
| dNTP mix (12.5 mM each) | 0.8 uL | 0.2 mM each dNTP |
| Primer A | Variable | 0.1-0.5 uM |
| Primer B | Variable | 0.1-0.5 uM |
| R-Taq DNA Pol | 1 mL | 5 units/reaction |
| Distilled Water | Variable | - - - - |
| Template DNA | Variable | 0.1-0.5 uG/reaction |
| TOTAL volume | 50 uL | - - - - |
Table 2. MgCl2 concentration
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.
4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturer's instructions.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.
Tween 20 is a registered trademark of ICI Americas, Inc.
| Final MgCl2 conc. in reaction (mM) | 1.5 | 2 | 2.5 | 3 | 3.5 | 4 | 4.5 |
| Vol. of 25 mM MgCl2 / rxn (uL) | 0 | 1 | 2 | 3 | 4 | 5 | 6 |
10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20
Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 uG EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.
Suggested Protocol using R-Taq Polymerase
This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.
1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.
Table 1. Reaction components (master mix & template DNA)
PR1MA™ Bromophenol Blue [3',3",5',5"-Tetrabromo-phenolsulfonphthalein]
Common Name: 3',3",5',5"-Tetrabromo-phenolsulfonphthalein
CAS Number: 115-39-9
Molecular Weight: 669.96
Chemical Formula: C19H10Br4O5S
Solubility: Methanol, ethanol
Storage Temp: Room Temperature
Research or further manufacturing use only, not for food or drug use.
- Appearance: Pink to Red Crystalline Powder
- Clarity of Solution: Pass
- Visual Transition Interval (pH 3.0 - 4.6): Pass
PR1MA™ DTT [DL-Dithiothreitol] (Cleland's Reagent)]
Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C4H10O2S2
Solubility: Water
Storage Temp: 2-8°C
Protective reagent for maintaining -SH groups in reduced state. DTT
permeates the cell membrane, protecting protein sulfhydryls, thereby
restoring enzyme activity lost by oxidation of the groups.
Research or further manufacturing use only, not for food or drug use.
- Appearance: A white free flowing powder, hygroscopic
- Solubility: Freely soluble in methanol, methylene chloride
- Clarity in water: 5% (w/v) solution in water is clear and colorless
- Identification by TLC: Rf value of the sample is comparable with that of the standard
- Melting Range: 37.0 - 44.0 °C
- Related Substance (UV Spectroscopy- Content of Oxidised DTT): 0.4 %
- Assay (By Iodimetry): ≥ 98.0 %
- Country of Origin: Report
PR1MA™ Ammonium Acetate
CAS Number: 631-61-8
Molecular Weight: 77.08
Chemical Formula: C2H7NO2
Solubility: Water
Storage Temp: 2-8°C
Widely used reagent in molecular biology and chromatography.
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline hygroscopic powder
- Assay: ≥ 97.0 %
- Chloride (Cl): ≤ 0.0005 %
- Heavy Metals (as Pb): ≤ 0.0005 %
- Insoluble Matter: ≤ 0.005 %
- Iron (Fe): ≤ 0.0005 %
- Nitrate: ≤ 0.001 %
- pH, 5% solution in H2O @25 ºC: 6.7 - 7.3
- Residue after Ignition: ≤ 0.01 %
- Sulfate: ≤ 0.001 %
PR1MA™ Temed
Common Name: N,N,N',N'-Tetramethylethylenediamine
CAS Number: 110-18-9
Molecular Weight: 116.2
Chemical Formula: C6H16N2
Solubility: Water
Storage Temp: Room Temperature
Tertiary amine used to catalyze formation of free radicals from ammonium persulfate and riboflavin. Suitable for electrophoresis and tested as a catalyst for polyacrylamide gel electrophoresis.
Shipping Weight: 0.4 lbs
Shipping Dimensions: 2.00 x 4.50 x 2.00
Research or further manufacturing use only, not for food or drug use.
- Appearance: Clear, Colorless Solution
- Identification: Conforms
- Purity (GC): ≥ 97 %
- BSE/TSE Free: BSE/TSE Free
- Country of Origin: Report
PR1MA™ Benzamidine Hydrochloride
CAS Number: 1670-14-0
Molecular Weight: 156.6
Chemical Formula: C7H8N2 • HCl
Solubility: Water
Storage Temp: 2-8°C
Competitive serine protease inhibitor which forms stable complexes with and blocks the active sites of enzymes. Target enzymes include trypsin, hymotrypsin, kallikzein and plasmin.
Shipping Weight: 0.3 lbs
Shipping Dimensions: 3.50 x 3.75 x 3.50
Research or further manufacturing use only, not for food or drug use.
Specifications
- Appearance: White crystalline powder
- Assay (HPLC): ≥ 98.5 %
- Loss on Drying: ≤ 2 %
- Residue on Ignition: ≤ 0.5 %
- Material Source: Chemical Synthesis
- BSE/TSE Free: BSE/TSE Free
- Country of Origin: Report
These items have been discontinued by the manufacturer.
See our MagBio HighPrep Plant DNA Plus Kits or contact us for more options.
PrecisionPak Plant Matter DNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, to ensure high quality DNA.
The PrecisionPak Plant Matter DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. The buffers' formulations are environmentally friendly and do not require ethanol, phenol, chloroform, and isopropanol. The extraction kit is innovatively designed to be faster and more efficient than spin-column methods.
Tough Plant Matter DNA Kit available for: Barley, Beans, Blueberry, Cannabis, Chickpeas, Cloves, Conifer Needles, Corn, Dried Soy Beans, Fungi (M.phaseolina), Ginger Rhizome, Horseradish Root, Leaves, Leek, Malanga (Tuber), Microsporidia, Moss, Mung Beans, Mycelia, N. benthamiana, Pine Nut, Potato, Rice, Soy Bean Plant, Spelt, Sugar Cane Pulp, Sunflower Seeds, Sorghum, Tobacco, Tomato Root, Tomato Seeds, Wheat Berries.
Soft Plant Matter DNA Kit available for: Algae, A thaliana, and Phytoplankton.
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