Home  >  Chemicals  >  Reagents

Reagents

Trusted, High‑Purity Reagents for Reliable Life Science Research

MIDSCI™ offers a wide selection of high‑quality laboratory reagents engineered for accuracy, reproducibility, and dependable performance. Whether you're running PCR, qPCR, ELISA, electrophoresis, or nucleic acid purification, our reagents are designed to help you collect consistent, high‑quality data. From enzymes and buffers to DNA ladders and biochemical components, every product meets strict purity and ISO‑aligned quality standards, giving your research the dependable foundation it deserves.

Our reagents support a wide range of molecular biology, microbiology, and biochemical applications. Designed to minimize impurities and enhance performance in sensitive assays, MIDSCI™ reagents help ensure clean results, reliable amplification, clear electrophoresis bands, and low‑background immunoassays. Whether you're working in an academic, clinical, or industrial lab, you’ll find the right tools to support your workflows.

Shop now for high-quality reagents or contact our experts for personalized recommendations.

Looking for ways to save? Sign up for our newsletter to hear about upcoming offers.

|◀ ◀ 73 - 84 of 139 ▶ ▶|
View:

MagBio HighPrep™ Plant RNA Plus Kit


Magnetic beads based kit designed to extract high-quality RNA from plants (leaves, roots, seeds).


Applications

Plant genomic RNA isolation for:
  • PCR, Real-time PCR
  • Cloning, genotyping
  • Sequencing
  • Plant breeding

Benefits
  • Purified PCR grade RNA with no inhibiting substances (polysaccharides and phenols)
  • Includes beads to homogenize sample
  • Eliminate need of grinding
  • Adaptable to automation

The HighPrep™ Plant RNA Plus Kit is specially designed for purifying RNA from a wide range of plant and fungi species. The kit uses the special lysis condition with HighPrep™ magnetic particles technology to isolate the high-quality RNA. Our kit's unique binding conditions ensure that only RNA will bind to the magnetic particles while most of the contaminating cellular proteinaceous components are removed. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, Southern blotting, SNP analysis and NGS etc. The HighPrep™ Plant RNA Plus Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Item#:
ASMAGBIOHIPLANTR

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
  • Tube volume: 3ml
  • Dialysis volume: 0.1-3ml
  • Min. sample size for extraction: 20µg
  • Max. gel slice: 2cm x 1cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 0.1-3ml
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification
Item#:
ASISOLAION2

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
  • Tube volume: 800µl
  • Dialysis volume: 50-800µl
  • Min. sample size for extraction: 0.5µg
  • Max. gel slice: 1cm x 0.5cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification.
Item#:
ASISOLAION4

MEGA Flex Tube Kit

MEGA Flex Tubes enable user friendly dialysis or buffer exchange of protein molecules with rapid, secure and simple loading along with high performance yield recovery.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 1K, 3.5K, 6-8K or 12-14K MWCO
  • Tube volume: 10ml, 15ml or 20ml
  • Dialysis volume: 3-20ml
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins

APPLICATIONS

  • Dialysis or buffer exchange with volumes between 3-20ml
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification
Item#:
ASISOLAION3

MINI Flex Tube Kit

MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

  • Flex Tubes 2/10/30/50/ 100 pieces
  • Supporting tray (for electro elution protocol) 1ea. (select kits)
  • Floating rack (for dialysis protocol) 1ea. (select kits)
  • Information and Protocol Manual 1ea.

SPECIFICATIONS

  • Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
  • Tube volume: 250µl
  • Dialysis volume: 10-250µl
  • Min. sample size for extraction: 0.5µg
  • Max. gel slice: 0.4cm x 1.1cm
  • Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
  • Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons

base pairs

1K

3 bp

3.5K

11bp

6-8K

18-24bp

12-14K

36-42bp

25K

76bp

50K

152bp

 

APPLICATIONS

  • Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis, such as antibodies and recombinant protein purification
  • Removal of contaminating micro-molecules
  • Tissue culture extraction purification
  • Removal of salts, surfactants, solvents, and detergents
  • Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
  • pH and buffer adjustment of sample solutions, protein extraction or cell extraction
  • High throughput dialysis
  • Peptide dialysis, as small as 10 amino acids
  • Virus-particles purification
Item#:
ASISOLAION5

MagBio CTL™ Medium (Collect - Transport - Lyse)


Applications
  • Sample collection, lysis, inactivation and stabilzation for - Viral, Fungal & Bacterial Samples (Swabs)
  • DNA & RNA sample preparation for pathogen detection
  • Flexibility in sample processing - Room Temperature Stability
  • DNA = 45 Days | RNA = 8 Days
  • COVID-19 Detection

Benefits
  • Significantly shortens sample preparation time in pathogen testing & detection workflows
  • Cost savings - Extraction & extraction accessories not required
  • Preserves and maintains the integrity of nucleic acids at room temperature:
  • DNA = 45 Days | RNA = 8 Days
  • Compatible with various extraction or purification workflows
  • Samples will be lysed during transport - No need for extraction / lysis.
  • Allows samples to be batched which can in turn improve the processing efficiency 
Time is always of the essence when it comes to pathogen detection and with MagBio CTL™, you can increase the efficiency and stability of your pathogen samples. Our Medium can be purchased in liters for bulk applications and can be integrated into a variety of workflows. Save time and money with MagBio CTL™.
Item#:
ASMAGBIOCTLMED

IBI MIDI I-Blue Plasmid Sample 

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

  • Sample: 1-7 mL of cultured bacterial cells
  • Yield: Up to 50 µg of pure plasmid DNA
  • Format: Plasmid spin column
  • Operation Time: Within 15 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Item#:
ASIBISAMPLEBLUE

MagBio HighPrep™ Plasmid DNA Plus Kit


Magnetic beads based kit designed to extract high-quality plasmid DNA (including Cosmids, Fosmids, and BACs) from bacterial cultures.


Applications

Plasmid DNA isolation for:

  •     PCR, Real-time PCR
  •     Cloning, genotyping
  •     Sequencing, NGS
  •     Transfection

Benefits

  •     High quality plasmid DNA purification
  •     High yields of both high and low copy number plasmids
  •     Adaptable to various automated liquid handling workstations
  •     No toxic organic solvents

The HighPrep™ Plasmid DNA kit is a magnetic beads-based plasmid purification system for high throughput rapid isolation of both high and low copy number plasmid DNA from bacterial cultures as well as for isolation of BACs, PACs, Cosmids or Fosmids.  The system can easily be automated on many liquid handling workstations, providing robustness and high-quality plasmid DNA for subsequent downstream applications such as restriction digestion, PCR, cloning and fluorescent DNA sequencing.
Item#:
ASHIHPRPPLADNAPK
You must be logged in to order this item. Log in here

The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Item#:
ASDNARNAKIT2

Sample Types:

Blood, cultured cells, body fluids, and other animal cells

Sample Size:

5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood
Format:
Spin columns
Operation:
Centrifuge/Vacuum

Binding Capacity:
Up to 60µg
Time:
30 minutes
Applications:

RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis
Item#:
ASDNARNAKIT1

Advantages

  • Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
  • Yield: Up to 5 µg of gDNA from fresh whole blood samples
  • Format: genomic DNA spin column
  • Operation Time: Within 20 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components


Component

IB47280

IB47281

IB47282

GST Buffer

3 mL

30 mL

75 mL

GSB Buffer

4 mL

40 mL

75 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer*
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL)

50 mL
(200 mL)

Proteinase K**
(Add ddH2O)

1 mg
(0.10 mL)

11 mg x 2
(1.10 mL)

65 mg
(6.50 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:
ASDNARNAKIT4
  • Sample: Gram (+) positive and Gram (-) negative bacterial cells
  • Yield: Up to 30 µg of gDNA - (1 x 10 Escherichia coli: 25-30 µg, 1 x 10 Bacillus subtilis: 10-15 µg)
  • Convenient: Includes Gram+ Buffer for preparing lysozyme solutions and to speed up sample preparation
  • Format: Genomic DNA spin columns
  • Operation Time: within 30 minutes
  • Elution Volume: 50-200 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

The gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

Quality Control
The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

Items

IB47290

IB47291

IB47292

Gram+ Buffer*

1.5 mL

30 mL

75 mL

GT Buffer

1.5 mL

30 mL

75 mL

GB Buffer

2 mL

40 mL

100 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer**
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL

50 mL
(200 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add lysozyme to Gram+ Buffer immediately prior to use. Once lysozyme is mixed with Gram+ Buffer, the solution can be stored for 2 weeks at 4°C.


**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

Item#:
ASDNARNAKIT3
|◀ ◀ 73 - 84 of 139 ▶ ▶|
View: