MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
• Tube volume: 800µl
• Dialysis volume: 50-800µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 1cm x 0.5cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification.

MEGA Flex Tube Kit

MEGA Flex Tubes enable user friendly dialysis or buffer exchange of protein molecules with rapid, secure and simple loading along with high performance yield recovery.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K, 3.5K, 6-8K or 12-14K MWCO
• Tube volume: 10ml, 15ml or 20ml
• Dialysis volume: 3-20ml
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins

APPLICATIONS

• Dialysis or buffer exchange with volumes between 3-20ml
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

MINI Flex Tube Kit

MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
• Tube volume: 250µl
• Dialysis volume: 10-250µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 0.4cm x 1.1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

Bullseye 1Kb DNA Ladder, Logic

• Ready to use
• Contains 16 DNA bands: 100bp-10Kb
• 920ng DNA/ 6 uL/loading
• Easy quantification of DNA fragments
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 1Kb DNA Ladder, Logic contains 16 DNA bands ranging from 100bp to 10Kb, formulated so that each band contains an amount of DNA that correlates logically to its size, allowing the user to estimate both the size and the quantity of specific fragments at a glance. It is particularly useful for protocols such as probe labeling, DNA sequencing and optimizing insert/vector ratio in ligation reactions, where DNA concentration must be taken into consideration.

Size: 1200 uL
Storage: Store at -20°C.
Concentration: 920 ng/6 uL

Loading Buffer Composition:

• 10mM Tris-HCl
• 1mM EDTA (pH 8.0)
• 0.02% Bromophenol blue
• 0.02% Xylene cyanol
• 5% Glycerol

Usage: Add 6 uL of Bullseye 1Kb DNA Ladder, Logic directly to wells designated for markers, which will yield the amount as indicated in the picture. You may use more than 6 uL of ladder, depending on well size and level of dye used to visualize the bands. Calculate the amount for each band according to your loading volume.

This brand is being discontinued.

Need a great DNA Ladders at a great price?

Try our PR1MA™ SmartCheck DNA Ladders!

Bullseye 100bp DNA Ladder RTU (Ready-to-Use)

• A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
• The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
• The approximate mass of DNA in each band is provided (0.5 ug a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source:

• PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 ug / mL
Package: 50 ug / 500 uL
Recommended Load: 5 uL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)

Storage:

• Store at 25°C for 6 months
• Store at 4°C for 12 months
• Store at -20°C for 24 months

Bullseye 100bp DNA Ladder

• Ready to use
• Contains 11 DNA bands: 100-1500bp.
• Clearly identifiable 500bp band as reference
• 500ng DNA/6 uL/loading
• Easy to load
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6 uL will yield at least 30 ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.

Size: 1200 uL
Storage: Store at -20°C.
Concentration: 500 ng/6 uL

Loading Buffer Composition:

• 10mM Tris-HCl
• 1mM EDTA (pH 8.0)
• 0.02% Bromophenol blue
• 0.02% Xylene cyanol
• 5% Glycerol

Usage: Add at least 6 uL Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6 uL of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.

MagBio CTL™ Medium (Collect - Transport - Lyse)

, Beads

IBI MIDI I-Blue Plasmid Sample 

MagBio HighPrep™ Plasmid DNA Plus Kit

, Beads

The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Advantages

• Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
• Yield: Up to 5 µg of gDNA from fresh whole blood samples
• Format: genomic DNA spin column
• Operation Time: Within 20 minutes
• Elution Volume: 30-100 µL
• Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.