Azure Chemi Blot Blocking Buffer

This Chemi Blot Blocking Buffer was formulated to enhance signal and lower background by improving sensitivity. Enhances specific antibody-antigen interactions specifically for chemiluminescent Western blots. 

• Provided as a 1x ready-to-use solution- no mixing or dilutions required.
• Size: 500mL

Features

Western blot membranes should be incubated in blocking buffer before antibody hybridization to prevent non-specific binding of antibodies. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.

Azure Blot Washing Buffer

Wash buffers are an important part of the blotting process for removing unhybridized antibody and preventing background signal.

Blot washing buffer for use with Azure Imaging Systems, or any other Western blotting system.

• Provided as a 10x concentrated solution
• Size: 500mL

NOTE: While this Blot Washing Buffer is compatible with NIR Fluorescent Western blots, for the best results with NIR fluorescence, we recommend the Azure Fluorescent Blot Washing Buffer.

Azure Protein-free Blot Blocking Buffer

Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots

Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.

Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.

• Provided as a 5x solution
• Size: 200mL

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Citric Acid, Monohydrate 

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals
• Purity: 99.0 - 102.0%
• Identification (FTIR): Conforms to Structure
• Water: 7.5 - 8.8% 
• Lead: ≤ 3 ppm
• Iron: ≤ 3 ppm
• Oxalate: To Pass Test
• Sulfate: ≤ 20 ppm
• Chloride: ≤ 10 ppm
• Readily Carbonizable Substances: Passes Test
• Insoluble Matter: ≤ 0.005%
• Residue After Ignition: ≤ 0.02%
• DNase: None Detected
• RNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

Citric Acid, Anhydrous

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals or Crystalline Powder
• Purity: ≥ 99.5%
• Identification (FTIR): Conforms to Structure
• Water: ≤ 0.3%
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Oxalate: ≤ 100 ppm
• Sulfate: ≤ 150 ppm
• Chloride: ≤ 50 ppm
• Barium: Passes Test
• Residue on Ignition: ≤ 0.05%
• Readily Carbonizable Substances: Passes Test
• Bacterial Endotoxins: ≤ 0.5 IU/mg
• DNase: None Detected
• RNase: None Detected 

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.

Tris Hydrochloride

• Appearance: White, Crystalline Powder
• Purity: ≥ 99.0%
• pH (1% Aqueous): 4.2 - 5.0
• Solubility (1M Aqueous): Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4h): ≤ 0.5%
• Melting Point: 149ºC - 153ºC
• A260 (40% Aqueous): ≤ 0.06
• A280 (40% Aqueous): ≤ 0.05
• Lead: 5 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Sodium Phosphate Dibasic, Heptahydrate

• Appearance: White to Off-White Powder
• Purity: ≥ 99.0%
• pH (5% Aqueous) : 8.7 - 9.3
• Solubility: Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4 h): 44 - 50%
• Lead: ≤ 10 ppm
• Iron: ≤ 10 ppm
• Chloride: ≤ 10 ppm
• Phosphate: 35.0% - 39.-%
• Sulfate: ≤ 50 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Sodium Phosphate Monobasic, Anhydrous

• Appearance (Color): White to Off-White
• Appearance (Form): Crystalline Powder
• Purity: 98.0 - 103.0%
• pH (5% Aqueous): 4.1 - 4.5
• Solubility: Clear and colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4h): ≤ 0.5%
• Insoluble Matter: ≤ 2.0%
• Lead: ≤ 5 ppm
• Arsenic: ≤ 3 ppm
• Fluoride: ≤ 50 ppm
• Phosphorous: 24.5 - 26.5%
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

Tris Base

• Appearance: White, Crystalline Powder
• Purity: ≥ 99.5%
• pH (5% Aqueous): 10.0 - 11.5
• Solubility (40% Aqueous): Clear and Colorless
• Identity (FTIR): Conforms to Structure: ≤ 0.5%
• Loss on Drying (105°C/4h): ≤ 0.5%
• Melting Point: 168ºC – 172ºC
• A280 (40% Aqueous): ≤ 0.07
• A400 (40% Aqueous): ≤ 0.02
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Chloride: ≤ 3 ppm
• Sulfate: ≤ 10 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Sodium Chloride

• Appearance: White Crystalline Powder
• Purity: 99.0 - 101.5%
• pH (5% Aqueous): 5.0 - 9.0
• Lead: ≤ 5 ppm
• Iron: ≤ 2 ppm
• Insoluble Matter: ≤ 0.005%
• Iodide: ≤ 20 ppm
• Bromide: ≤ 100 ppm
• Phosphate: ≤ 5 ppm
• Sulfate: ≤ 40 ppm
• Barium: To Pass Test
• Calcium: ≤ 20 ppm
• Magnesium: ≤ 10 ppm
• Potassium: ≤ 50 ppm
• Chlorate & Nitrate: ≤ 30 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.