MagBio HighPrep Plasmid DNA Plus Kit

MagBio HighPrep™ Plasmid DNA Plus Kit

Magnetic beads based kit designed to extract high-quality plasmid DNA (including Cosmids, Fosmids, and BACs) from bacterial cultures.

Applications

Plasmid DNA isolation for:

PCR, Real-time PCR
Cloning, genotyping
Sequencing, NGS
Transfection

Benefits

High quality plasmid DNA purification
High yields of both high and low copy number plasmids
Adaptable to various automated liquid handling workstations
No toxic organic solvents

The HighPrep™ Plasmid DNA kit is a magnetic beads-based plasmid purification system for high throughput rapid isolation of both high and low copy number plasmid DNA from bacterial cultures as well as for isolation of BACs, PACs, Cosmids or Fosmids. The system can easily be automated on many liquid handling workstations, providing robustness and high-quality plasmid DNA for subsequent downstream applications such as restriction digestion, PCR, cloning and fluorescent DNA sequencing.

Item#:

ASHIHPRPPLADNAPK

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DNase 1

The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Item#:

ASDNARNAKIT2

IBI Total RNA Kits (Cultured Cell and Blood)

Sample Types:	Blood, cultured cells, body fluids, and other animal cells
Sample Size:	5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood
Format:	Spin columns
Operation:	Centrifuge/Vacuum
Binding Capacity:	Up to 60µg
Time:	30 minutes
Applications:	RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis

Item#:

ASDNARNAKIT1

gMax Mini Kit

Advantages

Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
Yield: Up to 5 µg of gDNA from fresh whole blood samples
Format: genomic DNA spin column
Operation Time: Within 20 minutes
Elution Volume: 30-100 µL
Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 Î¼L of whole human blood. The purified DNA (5 Î¼g with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components

Component	IB47280	IB47281	IB47282
GST Buffer	3 mL	30 mL	75 mL
GSB Buffer	4 mL	40 mL	75 mL
W1 Buffer	2 mL	45 mL	130 mL
Wash Buffer* (Add Ethanol)	1 mL (4 mL)	25 mL (100 mL)	50 mL (200 mL)
Proteinase K** (Add ddH2O)	1 mg (0.10 mL)	11 mg x 2 (1.10 mL)	65 mg (6.50 mL)
Elution Buffer	1 mL	30 mL	75 mL
GD Columns	4	100	300
2 mL Collection Tubes	8	200	600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:

ASDNARNAKIT4

IBI gBAC Genomic DNA Kits

Sample: Gram (+) positive and Gram (-) negative bacterial cells
Yield: Up to 30 µg of gDNA - (1 x 10 Escherichia coli: 25-30 µg, 1 x 10 Bacillus subtilis: 10-15 µg)
Convenient: Includes Gram+ Buffer for preparing lysozyme solutions and to speed up sample preparation
Format: Genomic DNA spin columns
Operation Time: within 30 minutes
Elution Volume: 50-200 µL
Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

The gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

Quality Control
The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

Items	IB47290	IB47291	IB47292
Gram+ Buffer*	1.5 mL	30 mL	75 mL
GT Buffer	1.5 mL	30 mL	75 mL
GB Buffer	2 mL	40 mL	100 mL
W1 Buffer	2 mL	45 mL	130 mL
Wash Buffer** (Add Ethanol)	1 mL (4 mL)	25 mL (100 mL	50 mL (200 mL)
Elution Buffer	1 mL	30 mL	75 mL
GD Columns	4	100	300
2 mL Collection Tubes	8	200	600

*Add lysozyme to Gram+ Buffer immediately prior to use. Once lysozyme is mixed with Gram+ Buffer, the solution can be stored for 2 weeks at 4°C.

**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

Item#:

ASDNARNAKIT3

IBI Gel/PCR Fragment Extraction Kits

The Large DNA Fragments Extraction Kit was designed to recover or concentrate DNA fragments (> 8 Kb) from agarose gel in 4 easy steps. Salts and enzymes can be effectively removed from the reaction mixture without phenol extraction. Typically, recoveries are up to 85% for Gel Extraction. The entire procedure can be completed in 45 minutes and the DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

Specifications

Sample	Agarose Gel
Recovery	up to 85%
Format	spin column
Operation time	45 minutes

Item#:

ASDNARNAKIT9

IBI Gel/PCR Fragment Extraction Kits

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:	up to 300mg agarose gel slice up to 100µl PCR product or other enzymatic reaction
Format:	Spin columns
Operation:	Centrifuge/Vacuum Manifold
Binding Capacity:	10µg DNA
Expectant Yield:	80-90% for gel extraction 90-95% for PCR clean-up
Operational Time:	15min. for PCR clean-up/20min. for gel extraction
Applications:	PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

Item#:

ASDNARNAKIT6

IBI gYeast Genomic DNA Kits

Advantages

Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
Yield: Up to 5 µg of gDNA from fresh whole blood samples
Format: genomic DNA spin column
Operation Time: Within 20 minutes
Elution Volume: 30-100 µL
Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 Î¼L of whole human blood. The purified DNA (5 Î¼g with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components

Component	IB47280	IB47281	IB47282
GST Buffer	3 mL	30 mL	75 mL
GSB Buffer	4 mL	40 mL	75 mL
W1 Buffer	2 mL	45 mL	130 mL
Wash Buffer* (Add Ethanol)	1 mL (4 mL)	25 mL (100 mL)	50 mL (200 mL)
Proteinase K** (Add ddH2O)	1 mg (0.10 mL)	11 mg x 2 (1.10 mL)	65 mg (6.50 mL)
Elution Buffer	1 mL	30 mL	75 mL
GD Columns	4	100	300
2 mL Collection Tubes	8	200	600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:

ASDNARNAKIT5

Bullseye dNTP Mix - 12.5 mM

Discontinued, Contact us for more options!

This brand is being discontinued, please contact us for other options.

Need great dNTPs at a great price? Try our PR1MA™ dNTPs

Bullseye dNTP Mix - 12.5 mM

Mix of dATP, dCTP, dGTP, dTTP
Each nucleotide is at a concentration of 12.5 mM
Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
High purity: >98% by HPLC
Supplied in solution at pH 7.5
dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
Functionally tested with thermostable polymerases

Item#:

ASPCRDNTP1

EDTA Disodium Salt

Appearance: White Crystalline Powder
Purity: ≥ 99.0%
pH (5% Aqueous): 4.0 - 6.0
Solubility: Clear and Colorless to Faint Yellow
Identification (FTIR): Conforms to Structure
Loss on Drying: 1.0%
Insoluble Matter: ≤ 0.005%
Nitriloacetic Acid: ≤ 0.1%
Lead: ≤ 5 ppm
Iron: 10 ppm
DNase: None Detected
RNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.

Item#:

ASEDTADS

Bullseye dNTP Set

Discontinued, Contact us for more options!

This brand is being discontinued.

Need great dNTPs at a great price?

Try our PR1MA™ dNTPs

Bullseye dNTP Set

Mix of dATP, dCTP, dGTP, dTTP
Each nucleotide is at a concentration of 100 mM
Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
High purity: > 98% by HPLC
Supplied in solution at pH 7.5
dNTPs are stable at - 20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
Functionally tested with thermostable polymerases

dNTP SET

(dATP, dCTP, dGTP, dTTP)

100 mM

Item #	Size
BE511109	20 x 4 x 250 uL
BE511120	4 x 2 mL

Store at 20°C For in-vitro laboratory use only

Components Volume

dATP (100mM) 250 µL

dCTP (100mM) 250 µL

dGTP (100mM) 250 µL

dTTP (100mM) 250 µL

General Description

Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes.

Features

High purity: >98% by HPLC.

Supplied in solution at pH 7.5.

Storage Conditions

dNTPs are stable at 20oC in a constant temperature freezer. Avoid multiple freeze/thawing. For long-term usage, aliquoting is recommended.

Quality control

Functionally tested with thermostable polymerases.

Item#:

ASPCRDNTP3

Bullseye dNTP Mix - 10 mM

Discontinued, Contact us for more options!

This brand is being discontinued, please contact us for other options.

Need great dNTPs at a great price? Try our PR1MA™ dNTPs

Bullseye dNTP Mix - 10 mM

Mix of dATP, dCTP, dGTP, dTTP
Each nucleotide is at a concentration of 10 mM
Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
High purity: >98% by HPLC
Supplied in solution at pH 7.5
dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
Functionally tested with thermostable polymerases

Item#:

ASPCRDNTP2

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