PR1MA™ Expanded Surface Tissue Culture Treated Roller Bottles

Designed to maximize cell growth efficiency while saving valuable lab space.

Featuring a uniform hydrophilic surface, these ESRB bottles support consistent cell attachment and growth, making them ideal for a wide range of cell culture applications. Available in multiple sizes and cap configurations, including vented and non-vented options, these roller bottles provide flexibility for both open and closed systems.

• Increase productivity with larger surface areas
• Reduce handling time and repetitive tasks
• Optimize laboratory space with high-capacity bottle options
• Maintain consistent and reliable cell growth conditions

Main Attributes

• Expanded surface (ESRB) design maximizes cell culture area and efficiency
• Horizontal ribbing promotes more even cell exposure compared to vertical rib designs
• Tissue culture treated surface ensures a uniform hydrophilic surface for reliable cell attachment
• Large knurled caps for easy handling and secure grip
• Vented and non-vented cap options for application flexibility
• Medical-grade virgin polystyrene construction for consistent performance
• Easy-to-read black printed graduations for accurate measurement
• Sterile, individually bagged packaging for immediate use 

Available Configurations

• 1900 cm² Expanded Surface Roller Bottles
• Available with non-vented or vented caps
• Expanded surface design increases available growth area
• Vented cap option includes a 0.22 uM hydrophobic membrane for gas exchange in open systems
• 4250 cm² Expanded Surface Roller Bottles
• Designed for large-scale cell culture applications
• Provides the equivalent growth area of five standard 850 cm² bottles while using 40% less plastic
• Fits into roller apparatuses that accommodate two standard 850 cm² bottles

Same Reliable Product, Now with Updated Part Numbers

PR1MA™ Tissue Culture Treated Roller Bottles

Sterile, vented, and non-vented options.

PR1MA™ Tissue Culture Treated Roller Bottles are designed to deliver reliable and consistent performance for cell culture applications. With a uniform hydrophilic surface, these bottles promote optimal cell attachment and growth, making them ideal for a wide range of laboratory workflows. Available in 490 cm² and 850 cm² sizes with both vented and non-vented cap options, these roller bottles provide flexibility for closed and open system applications.

• Supports consistent and reproducible cell culture results
• Flexible options for different workflows and system requirements
• Easy handling and reliable performance in laboratory environments
• Multiple sizes to accommodate varying culture volumes 

Main Attributes

• Tissue culture treated surface provides a uniform hydrophilic environment for consistent cell attachment
• Large knurled caps ensure easy handling and secure grip
• Vented cap options designed for open system applications with 0.22 uM hydrophobic membrane
• Medical-grade virgin polystyrene construction ensures quality and consistency
• Easy-to-read black printed graduations for accurate measurement
• Sterile packaging in individual bags or bulk sterile bags for convenience and protection 

Available Configurations

• 490 cm² Roller Bottles
• Vented (PR-RBVC-490) and non-vented (PR-RBNVC-490) cap options
• Vented caps include a 0.22 uM hydrophobic membrane for gas exchange
• 850 cm² Roller Bottles
• Vented (PR-RBVC-850) and non-vented (PR-RBNVC-850) cap options
• Vented versions support open system culture with membrane filtration
• Available in bulk (PR-RBVC-850-40): 20 bottles per sterile vacuum bag with dual dust bag layers, 2 bags per case

PR1MA™ Non-Treated Roller Bottles

Non-treated roller bottles with sterile, non-vented cap.

PR1MA™ Non-Treated Roller Bottles are designed for reliable cell culture applications requiring non-treated surfaces. Manufactured from medical-grade virgin polystyrene, these roller bottles provide consistent performance and durability while maintaining sterility with individual packaging.

Main Attributes

• Non-treated surface ideal for suspension cultures or specialized cell applications
• Non-vented cap ensures a secure seal during use
• Large knurled cap provides easy handling and improved grip
• Medical-grade virgin polystyrene construction for consistency and quality
• Easy-to-read black printed graduations allow accurate volume measurement
• Individually bagged and sterile for immediate use in laboratory environments 

Cell Culture Guidelines

• Recommended seeding density: 1 x 10³ to 1 x 10⁵ cells/cm²
• Initial adhesion phase (first 24 to 48 hours):
• Rotation speed: 0.5 to 1.0 rpm
• For cells with lower adhesion:
• Rotation speed: 0.3 to 0.5 rpm

*Note: These parameters are general recommendations; optimize conditions based on specific cell lines.

Cell Harvesting Protocol

• Rinse the bottle twice with PBS
• Add a dissociation solution (e.g., trypsin), ensuring full coverage of the bottle interior and pleats
• Place the bottle in an incubator and rotate at 0.3 to 0.5 rpm for 5 to 10 minutes
• After cell detachment, add an equal volume of cell culture medium to neutralize trypsin
• Rotate the bottle to collect the cell suspension
• Remove cells by pouring or pipetting