PR1MA™  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g / L
• Sodium Chloride: 10 g / L
• Yeast Extract: 5 g / L
• Agar: 12 g / L

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, none to light ppt, light yellow
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2% water) @25°C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Casein Peptone: 10 g/L

PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant proteins. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.

Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Terrific Broth Modified Capsules

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Terrific Broth formulation packaged in easy to use 24 gram capsules. Formulation from powder.

Add 1 capsule per 500 mL of water. Heat and stir until capsules are completely dissolved. Add 2ml of glycerol to mixture. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Casein Digest Peptone: 12 g/L
• Yeast Extract: 24 g/L
• Dipotassium Phosphate: 9.4 g/L
• Potassium Phosphate: 2.2 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Water soluble gelatin capsules containing cream to beige free flowing powder.
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH (4.76% solution at 25°C): 7.0 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Yeast Extract: 24.0 g/L
• Casein Peptone: 12.0 g/L
• Potassium phosphate dibasic: 9.4 g/L
• Potassium phosphate monobasic: 2.2 g/L
• Direction (Preparation): Add 2 capsules to one liter of purified water until evenly dispersed. Add 4ml of Glycerol to the mixture. Boil for one minute with stirring to completely dissolve. Distribute and autoclave at 121°C for 15 minutes.

PR1MA™ LB Broth 20 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

Now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. Low Salt Formula. Molecular Biology Grade.

For propagation and maintenance of E.Coli:

• Add 1 capsule per liter of water.
• Heat and stir until capsule is completely dissolved.
• Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl
• Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Nematode Growth Medium, Powder

Common Name: Nematode Growth Medium

Solubility: Water with Heat

Storage Temp: Room Temperature

A standard formulation for use in Nematode growth studies.

Contains:

Agar 17.5 g/l
Sodium Chloride 3.0 g/l
Peptone 2.5 g/l
Cholesterol 0.005 g/l
Shipping Dimensions: 3.50 x 3.75 x 3.50
Shipping Weight: 1.2 lbs

Research or further manufacturing use only, not for food or drug use. 

Specifications

• Appearance: Light tan to pale yellow powder
• Solubility: Clear Solution
• pH (2.3% Solution @25 oC): Report
• Agar: 17.5 g/l
• Sodium Chloride: 3.0 g/l
• Peptone: 2.5 g/l
• Cholesterol: 0.005 g/l

PR1MA™ SOB Broth, Powder

Solubility: Water
Storage Temp: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.

Add 28 grams of Broth in 1 liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes.

Ingredients:

• Casein Enzymic Hydrolysate: 20 g/L
• Magnesium Sulfate: 2.4 g/L
• Yeast Extract: 5 g/L
• Potassium Chloride: 0.186 g/L
• Sodium Chloride: 0.5 g/L
• Final pH(25°:C): 7.0+0.2

• DCM Appearance: Homogeneous Free Flowing,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.8 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Miller's LB Agar Capsules

PR1MA™ LB Broth 1 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

Now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. For propagation and maintenance of E.Coli. Low Salt Formula. Molecular Biology Grade.

Add 20 capsules per liter of water. Heat and stir until capsule is completely dissolved. Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.15% solution @25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ NZCYM Broth

Solubility: Water
Storage Temp: Room Temperature

Enriched medium for cultivating recombinant strains of E.Coli and propagating theta bacteriophage.

Dissolve 22 grams in 1 liter distilled water. Sterilize by autoclaving for 15 minutes.

Casein Digest Protein: 10 g/L
Yeast Extract: 5 g/L
Casamino Acids: 1 g/L
Magnesium Sulfate: 0.98 g/L
Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2.2 % Solution): 6.8 - 7.2
• Escherichia coli ATCC 23724: Good to Excellent
• Escherichia coli ATCC 33694: Good to Excellent
• Escherichia coli ATCC 33849: Good to Excellent
• Escherichia coli ATCC 39403: Good to Excellent
• Escherichia coli ATCC 47014: Good to Excellent
• Escherichia coli ATCC 53868: Good to Excellent
• Country of Origin: Report

PR1MA™ Nutrient Broth

Solubility: Water
Storage Temp: Room Temperature

For cultivating a variety of non-fastidious organisms.

Dissolve 8 grams in one liter distilled water. Heat solution to boiling with continuous stirring for one minute. Sterilize by autoclaving at 121°C for 15 minutes. Store at room temperature.

Beef Extract: 3 g/L
Pancreatic Digest of Gelatin: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous Free Flowing,Beige Powder
• pH (0.8 % Solution @25 ^oC): 6.6 - 7.0
• Microorganism (Bacillus Subtilis ATCC 9372): Fair to Good
• Microorganism (Escherichia Coli ATCC 25922): Good to Excellent
• Microorganism (Salmonella Typhimurium ATCC 14028 ): Fair to Excellent
• Microorganism (Staphylococcus Aureus ATCC 25923): Good to Excellent
• Pancreatic digest of gelatin: 5.0 g/L
• Beef Extract: 3.0 g/L

PR1MA™ Nutrient Agar

Solubility: Water with Heat

Storage Temp: Room Temperature

For cultivating a variety of non-fastidious organisms.

Dissolve 23 g in one liter of distilled water. Heat the solution to a boil with continuous stirring for one minute. Sterilize by autoclaving at 121°C for 15 minutes. Mix the medium with gentle agitation to avoid bubbles prior to pouring plates. Store at room temperature.

Ingredients

• Beef Extract: 3 g/L
• Pancreatic Digest of Gelatin: 5 g/L
• Shipping Dimensions: 3.50 x 7.00 x 3.50
• Agar: 15 g/L
• Shipping Weight: 1.2 lbs

*Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous Free Flowing, Beige
• PPM Appearance: Trace to Light Haze, None to Light PPT, Light Beige Amber
• pH (2.3% Solution @25 oC): 6.6 - 7.0
• Bacillus Subtilis ATCC 9372: Good to Excellent
• Microorganism (Escherichia Coli ATCC 25922): Good to Excellent
• Microorganism -Pseudomonas Aeruginosa ATCC 27853: Good to Excellent
• Microorganism (Staphylococcus Aureus ATCC 25923): Good to Excellent
• Salmonella Typhimurium ATCC 14028: Good to Excellent