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PR1MA™ β - Agarase

 

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.


Benefits

  • Complete digestion of agarose, with no agarose fragments, left after the digestion
  • Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
  • Concentration: 1000 u / mL
  • Operating temperature: 42°C to 50°C
  • Recommended temperature: 50°C
  • Storage temperature: -20°C 

Storage Buffer

  • 50% glycerol
  • 50 mM Tris-HCI
  • 50 mM KCI
  • 1 mM DTT
  • 0.1  mM EDTA
  • pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.


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PR1MA™ T4 Gene 32 Protein

 

PR1MA™ T4 Gene 32 Protein (T4 gp32) is a single-stranded DNA binding (ssDNA) protein required for E. coli bacteriophage T4 replication. It binds and stabilizes ssDNA structures which facilitates electron microscopic examination, and has also been shown to improve restriction digests, improve T4 DNA polymerase activity, and increase the yield of PCR reactions, including those with long amplicons. 

  • Optimal temperature: 37°C
  • Heat inactivation: 65°C for 20 minutes
  • Storage temperature: -20°C
  • 10X T4 gp32 reaction buffer included

Buffer composition

  • 50% glycerol
  • 50 mM Tris-HCl
  • 50 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • 0.1% Tween-20
  • pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established. 
 

Proudly made in the USA! Click here for more made in America products!


2026 On Target Promo!

 

For a limited time, buy one Accuris™ Fast Extraction PCR Kit,
High Fidelity Hot Start Red Dye Master Mix, 100 Reactions
(PR1400-FE-100), and get ONE FREE (PR1400-FE-100-VF).

  

For free product, add item PR1400-FE-100 at $127.53

PLUS (1) item PR1400-FE-100-VF to your cart.

 

Use promo code: OT202

 

Click here for more On Target savings

or see flyer for more details.

 

Promotion valid until 10/31/26 or while supplies last, for eligible U.S. customers.

Prices are in U.S. dollars and exclude freight, taxes, installation, and other applicable charges.

Offers may not be combined with other offers, discounts, or institutional pricing.

Customer must pay the list price on the qualifying item to receive the free item (List Price is reflected in flyer).

Buy-one-get-one offers apply to identical eligible catalog numbers unless otherwise stated.

All terms and conditions apply.


Accuris™ Fast Extraction PCR Kit

 

The Accuris™ Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

 

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

  • Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
  • Compatible with animal tissue, plant, saliva, & bacterial samples
  • Extraction & Amplification in less than 60 minutes
  • Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
  • Process extracted sample via PCR with included mastermix
  • Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

 

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

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PR1MA™ Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Purified agar with excellent properties for tissue culture and microbiological work.

  • To prepare media, add 15 grams to 1 liter of water.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Cream Color, Average Particle Size Between 1-2 mm
  • Granulometry (ASTM) 60 Mesh Sieve Size: Report %
  • Ash: ≤ 6.5 %
  • E.Coli: Negative
  • Gel Point (1.5% Gel): 33 - 38°C
  • Gel Strength (1.5% Gel): 600 - 1100 g/cm2
  • Melting Point: 88 - 96°C
  • Moisture : ≤ 15 %
  • Molds and Yeasts: < 100 cfu/g
  • pH: 6.0 - 8.0
  • Total Plate Count: < 1000 cfu/g
  • Viscosity (1.5 Solution @ 60 oC): ≤ 30 cps
  • Water Absorption: ≤ 75 cc

Proudly made in the USA! Click here for more made in America products!

PR1MA™ Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.

Gel Strength, 4%: ≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Powder
  • EEO: ≤ 0.13 -Mr
  • Gelling Temperature: 32.5 - 38.0 oC
  • Gel Strength : ≥ 1400 g/cm2
  • Melting Temperature: ≤ 90.0 oC
  • Moisture Content: ≤ 10.0 %
  • Sulfate: ≤ 0.15 %


Proudly made in the USA! Click here for more made in America products! 

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.

Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to off white powder
  • DNase/ RNase: Not Detected
  • EEO: ≤ 0.10 -Mr
  • Gelling Temperature: 26 - 30 oC
  • Gel Strength : ≥ 200 g/cm2
  • Melting Temperature: ≤ 65 oC
  • Moisture Content: ≤ 10 %
  • Sulfate Content: ≤ 0.10 %

Proudly made in the USA! Click here for more made in America products!  


Vion Ultrapure ASTM Type I Water

 

Our ultrapure, ASTM Type I, water meets the strictest standards defined by the American Society for Testing and Materials (ASTM). It is ideal for a wide range of ultra-sensitive laboratory uses include gas chromatography, HPLC, mass spectrometry, atomic absorption spectroscopy, and trace analysis.

 

Specifications
 
Resistivity≥ 18 MΩ
Conductivity≤ 0.056 uS / cm
Total Organic Carbon (TOC)≤ 50 ppb
Silicate (as SiO2)≤ 3 ppb
Chloride (Cl)≤ 1 ppb
Sodium (Na)≤ 1 ppb
Endotoxin≤ 0.03 EU / mL
Heterotrophic BacteriaCount (HBC)≤ 10 CFU / L
 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASASTMWATER

Accuris qMAX SYBR Green Bulk Packaging


Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fast cycling and excellent reproducibility.
  • Superior sensitivity and fast cycling with exceptional results.
  • Ideal for low copy number templates.
  • Early Ct values and detection across a broad dynamic range.
  • Ready to use 2x mastermix.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers. Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs.

Proudly made in the USA! Click here for more made in America products!


Vion ACS Reagent Grade Water

 

Vion ACS Reagent grade water is guaranteed to meet the purity standards set by the American Chemical Society (ACS). It is a high-quality, highly purified water with minimal contaminants, and is ideal for use in laboratory settings for sensitive chemical analyses, like liquid chromatography and UV-Visible spectrophotometry, and reactions where extreme purity is required.

 

Specifications
 
Conductivity≤ 2.0 uS / cm
Silicate (as SiO2)≤ 0.01 ppm
Heavy Metals (as Pb)≤ 0.01 ppm
Substances Reducing PermanganatePasses Test
Chloride (Cl)≤ 0.4 ppm
Nitrate (NO3)≤ 0.4 ppm
Phosphate (PO4) ≤ 1 ppm
Sulfate (SO4)≤ 1 ppm
UV A300 nm≤ 0.005
UV A254 nm≤ 0.005
UV A210 nm≤ 0.010
UV A200 nm≤ 0.010
LC AbsorbancePasses Test
LC Gradient ElutionPasses Test
 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASACSWATER


Lab-Sorb™ Granules

 

Converts liquid waste to solid waste in seconds. LabSorb™ rapidly absorbs aqueous or mixed aqueous/organic solvent waste. Use at locations connected to septic systems to avoid pouring waste down the drain. Put LabSorb™ at the bottom of your biohazard bin. One jar of LabSorb™ can absorb up to 50 liters of liquid. Retains liquid during autoclaving.

 

Includes extra durable disposal bags, super absorbent LabSorb™ granules, and a convenient graduated scoop.

  • Lab-Sorb™ Kits: SORB-2000 (Small Jar), SORB-2100 (Medium Jar), and SORB-2200 (Large Jar) Contain 1 jar of LabSorb™, 25 small disposal bags, 25 twist-ties
  • Variety Kit Contents: 1 Small LabSorb™ Jar, 15 small disposal bags, 10 medium disposal bags, 5 large disposal bags, 25 twist-ties   

Proudly made in the USA! Click here for more made in America products!

Item#:
ASABSORBJARS


PR1MA™ bis-Acrylamide, 2% Solution

CAS Number: 110-26-9
Storage Temp: 2-8°C

A 2% (w/v) bis-Acrylamide solution.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear, colorless liquid
  • Conductivity of Solution: Report µmhos
  • Electrophoresis: Pass Test
  • pH: Report

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PR1MA™  Blasticidin S Hydrochloride 10 mg / mL Solution

CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C17H26N8O5 • HCl
Solubility: Water
Storage Temp: -20°C

A peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryates.

Supplied as a 10 mg / mL sterile solution in HEPES buffer.
Research or further manufacturing use only, not for food or drug use.
  • Appearance: Clear Solution
  • Concentration: 10mg/ml sterile solution
  • Description: Colorless solution in HEPES buffer
  • pH: 7.2 - 7.5
  • Purity: ≥ 98.0 %

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