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Accuris Fast Extraction PCR Kit

 

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

 

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

  • Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
  • Compatible with animal tissue, plant, saliva, & bacterial samples
  • Extraction & Amplification in less than 60 minutes
  • Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
  • Process extracted sample via PCR with included mastermix
  • Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

 

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

Proudly made in the USA! Click here for more made in America products!

 

PR1MA Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Purified agar with excellent properties for tissue culture and microbiological work.

  • To prepare media, add 15 grams to 1 liter of water.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Cream Color, Average Particle Size Between 1-2 mm
  • Granulometry (ASTM) 60 Mesh Sieve Size: Report %
  • Ash:  6.5 %
  • E.Coli: Negative
  • Gel Point (1.5% Gel): 33 - 38°C
  • Gel Strength (1.5% Gel): 600 - 1100 g/cm2
  • Melting Point: 88 - 96°C
  • Moisture :  15 %
  • Molds and Yeasts: < 100 cfu/g
  • pH: 6.0 - 8.0
  • Total Plate Count: < 1000 cfu/g
  • Viscosity (1.5 Solution @ 60 oC):  30 cps
  • Water Absorption: ≤ 75 cc

Proudly made in the USA! Click here for more made in America products!

PR1MA Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.

Gel Strength, 4%: ≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Powder
  • EEO:  0.13 -Mr
  • Gelling Temperature: 32.5 - 38.0 oC
  • Gel Strength :  1400 g/cm2
  • Melting Temperature:  90.0 oC
  • Moisture Content:  10.0 %
  • Sulfate:  0.15 %


Proudly made in the USA! Click here for more made in America products! 

Kappa is now PR1MA - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.

Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to off white powder
  • DNase/ RNase: Not Detected
  • EEO:  0.10 -Mr
  • Gelling Temperature: 26 - 30 oC
  • Gel Strength :  200 g/cm2
  • Melting Temperature:  65 oC
  • Moisture Content:  10 %
  • Sulfate Content:  0.10 %

Proudly made in the USA! Click here for more made in America products!  


Vion Ultrapure ASTM Type I Water

 

Our ultrapure, ASTM Type I, water meets the strictest standards defined by the American Society for Testing and Materials (ASTM). It is ideal for a wide range of ultra-sensitive laboratory uses include gas chromatography, HPLC, mass spectrometry, atomic absorption spectroscopy, and trace analysis.

 

Specifications
 
Resistivity≥ 18 MΩ
Conductivity≤ 0.056 uS / cm
Total Organic Carbon (TOC)≤ 50 ppb
Silicate (as SiO2)≤ 3 ppb
Chloride (Cl)≤ 1 ppb
Sodium (Na)≤ 1 ppb
Endotoxin≤ 0.03 EU / mL
Heterotrophic BacteriaCount (HBC)≤ 10 CFU / L
 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASASTMWATER

Accuris qMAX SYBR Green Bulk Packaging


Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fast cycling and excellent reproducibility.
  • Superior sensitivity and fast cycling with exceptional results.
  • Ideal for low copy number templates.
  • Early Ct values and detection across a broad dynamic range.
  • Ready to use 2x mastermix.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers. Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs.

Proudly made in the USA! Click here for more made in America products!


Vion ACS Reagent Grade Water

 

Vion ACS Reagent grade water is guaranteed to meet the purity standards set by the American Chemical Society (ACS). It is a high-quality, highly purified water with minimal contaminants, and is ideal for use in laboratory settings for sensitive chemical analyses, like liquid chromatography and UV-Visible spectrophotometry, and reactions where extreme purity is required.

 

Specifications
 
Conductivity≤ 2.0 uS / cm
Silicate (as SiO2)≤ 0.01 ppm
Heavy Metals (as Pb)≤ 0.01 ppm
Substances Reducing PermanganatePasses Test
Chloride (Cl)≤ 0.4 ppm
Nitrate (NO3)≤ 0.4 ppm
Phosphate (PO4) ≤ 1 ppm
Sulfate (SO4)≤ 1 ppm
UV A300 nm≤ 0.005
UV A254 nm≤ 0.005
UV A210 nm≤ 0.010
UV A200 nm≤ 0.010
LC AbsorbancePasses Test
LC Gradient ElutionPasses Test
 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASACSWATER


PR1MA bis-Acrylamide, 2% Solution

CAS Number: 110-26-9
Storage Temp: 2-8°C

A 2% (w/v) bis-Acrylamide solution.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear, colorless liquid
  • Conductivity of Solution: Report µmhos
  • Electrophoresis: Pass Test
  • pH: Report

Proudly made in the USA! Click here for more made in America products!  


PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 29:1 Ratio

Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Conductivity of Solution: ≤ 10.0 µmhos
  • Electrophoresis: Pass Test
  • Solubility: Pass Test

Proudly made in the USA! Click here for more made in America products!  


PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 37.5:1 Ratio

CAS Number: 79-06-1, 110-26-9
Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White powder
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • Solubility: Pass Test

Proudly made in the USA! Click here for more made in America products!  


PR1MA Acrylamide/bis-Acrylamide, 37.5:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C

A 40% solution containing 38.96% (w/v) Acrylamide and 1.04% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 37.5:1.

WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear, colorless solution
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • pH: Report

Proudly made in the USA! Click here for more made in America products!  


PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 19:1 Ratio

Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Powder
  • Solubility: Clear and complete
  • Conductivity:  10 µmhos

Proudly made in the USA! Click here for more made in America products!  

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