PR1MA™ Terrific Broth, Modified, Powder

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Enriched media for maintenance and propagation of E. coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant protein. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.

Ingredients:

• Casein Digest Peptone: 12 g/L
• Yeast Extract: 24 g/L
• Dipotassium phosphate: 9.4 g/L
• Monopotassium phosphate: 2.2 g/L

• DCM Appearance: Homogeneous Free Flowing, Beige to Yellow Beige
• PPM Appearance: Clear Yellow to Amber, No PPT
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™  Luria Broth, High Salt Formula, Powder [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. High Salt Grade. Molecular Biology Grade.

• Dissolve 25 grams in 1 liter distilled water
• Adjust the pH to 7.2
• Sterilize by autoclaving for 15 minutes

• Tryptone: 10 g/L
• Yeast: 5 g/L
• Sodium Chloride: 10 g/L

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear,no to light ppt, light yellow
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• BSE/TSE Free: BSE/TSE Free
• Country of Origin: USA

PR1MA™ Luria Broth 1 Gram Buffered Capsules [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1/1185-53-1
Solubility: Water
Storage Temp: Room Temperature

High salt formula. Molecular Biology Grade. For maintenance and propagation of E. Coli.

Now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments.

Simply dissolve 25 capsules per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.0 ± 0.2 at 25°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.

Ingredients:

• Tryptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L
• Tris/Tris HCl: 1.5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Growth
• Microorganism (E. coli ATCC 33694): Growth
• Microorganism (E. coli ATCC 33849): Growth
• Microorganism (E. coli ATCC 39403): Growth
• Microorganism (E. coli ATCC 47014): Growth
• Microorganism (E. coli ATCC 53868): Growth

PR1MA™ Lactobacillus MRS Broth Powder

Solubility: Water
Storage Temp: Room Temperature

Recommended for the selective isolation and enumeration of lactobacilli.

Ingredients:

• Peptic Digest of Animal Tissue: 10.0g
• Beef Extract: 10.0g
• Yeast Extract: 5.0g
• Ammonium Citrate: 2.0g
• Dextrose: 20.0g
• Polysorbate 80:1.0g
• Sodium acetate: 5.0g
• Magnesium Sulfate: 0.1g
• Manganese Sulfate: 0.05g
• Sodium Phosphate, Dibasic: 2.0g

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing with soft lumps, yellow beige to yellow tan
• PPM Appearance: Clear, no to light ppt, medium to dark amber
• Microorganism (Lactobacillus Casei ATCC 393 ): Good to Excellent
• Microorganism (Lactobacillus Fermentum ATCC 9338 ): Good to Excellent
• Microorganism (Lactobacillus Plantarum ATCC 8014 ): Good to Excellent
• Microorganism (Lactobacillus Rhamnosus ATCC 9595 ): Good to Excellent
• pH (5.5 % Solution @25 ^oC): 6.3 - 6.7