PR1MA™ Reverse Transcriptase, RNase H -

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.

• Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
• Lacks 3’ to 5’ exonuclease activity
• Optimal temperature at 42°C
• Temperature range: 40 to 50°C
• Heat inactivation: 70°C for 20 minutes
• Storage temperature: -20°C
• 10X reaction buffer included 

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

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PR1MA™ RNA Controls

Looking for safe assay controls for highly infectious viruses or foreign animal diseases?

MIDSCI™ offers a selection of PR1MA™ RNAs – nuclease-resistant, single-stranded RNAs, suitable as process controls for RNA extraction from various sample matrices. These specially engineered, non-infectious, MS2 phage-like particles protect their contents from degradation by nucleases and can package sequences of up to 1.5 kb from viruses such as SARS-CoV-2, foot-and-mouth disease virus, and human immunodeficiency virus.
 

Amount: Between 1E6 or 1E10 copies (cp)
Concentration: Between 1E7 or 1E10 cp / mL, respectively
Volume: 0.1 and 1 mL respectively
Lysis: 65° C for 5 minutes in the RT step or by standard RNA extraction
Storage temperature: 4°C

Buffer composition

• 10 mM Tris HCl
• 100 mM NaCl
• 1 mM MgCl2
• 0.1% gelatin
• pH = 7.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!

PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!

PR1MA™ Taq DNA Polymerase 1X Master Mix

PR1MA™ DNA polymerase 1X Master Mix saves time and cost by enabling direct PCR amplification of unpurified templates. It contains a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays and is provided as a complete reaction master mix consisting of reaction buffer, dNTPs, MgCl2, and loading dye and only requires the addition of primers and DNA template. Once PCR is complete, the reaction products can be loaded directly into an agarose gel for analysis.

• Lacks exonuclease activity
• Thermotolerant up to 98°C
• Inhibitor Resistant
• Ideal for colony PCR, genotyping, and GC-rich templates
• Storage temperature: -20°C 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!

PR1MA™ β - Agarase

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.

Benefits

• Complete digestion of agarose, with no agarose fragments, left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
• Concentration: 1000 u / mL
• Operating temperature: 42°C to 50°C
• Recommended temperature: 50°C
• Storage temperature: -20°C 

Storage Buffer

• 50% glycerol
• 50 mM Tris-HCI
• 50 mM KCI
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!  

PR1MA™ T4 Gene 32 Protein

PR1MA™ T4 Gene 32 Protein (T4 gp32) is a single-stranded DNA binding (ssDNA) protein required for E. coli bacteriophage T4 replication. It binds and stabilizes ssDNA structures which facilitates electron microscopic examination, and has also been shown to improve restriction digests, improve T4 DNA polymerase activity, and increase the yield of PCR reactions, including those with long amplicons. 

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C
• 10X T4 gp32 reaction buffer included

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.1% Tween-20
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established. 
 

Proudly made in the USA! Click here for more made in America products!

PR1MA™ Agar, High Gel Strength, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Purified high gel strength agar with excellent properties for tissue culture and microbiological work.

Molecular Biology Grade

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Cream Powder
• Ash: ≤ 4.5 %
• Gel Point (1.5% Solution): 33 - 38 °C
• Gel Strength (1.5% Gel): ≥ 800 g/cm²
• Loss on Drying: ≤ 12.0 %
• Melting Point (1.5 %): 88.0 - 96.0
• pH (1.5% solution): 6.0 - 8.0

PR1MA™ Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Purified agar with excellent properties for tissue culture and microbiological work.

• To prepare media, add 15 grams to 1 liter of water.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Cream Color, Average Particle Size Between 1-2 mm
• Granulometry (ASTM) 60 Mesh Sieve Size: Report %
• Ash: ≤ 6.5 %
• E.Coli: Negative
• Gel Point (1.5% Gel): 33 - 38°C
• Gel Strength (1.5% Gel): 600 - 1100 g/cm²
• Melting Point: 88 - 96°C
• Moisture : ≤ 15 %
• Molds and Yeasts: < 100 cfu/g
• pH: 6.0 - 8.0
• Total Plate Count: < 1000 cfu/g
• Viscosity (1.5 Solution @ 60 ^oC): ≤ 30 cps
• Water Absorption: ≤ 75 cc

PR1MA™  Agarose, Low EEO

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Molecular Biology Grade. High purity for preparative and analytical electrophoresis of nucleic acids >1000 bp.

Gelling Temperature: 34.5 -37.5°C
Sulfate: 0.15
EEO: 0.09- 0.13
DNA Binding: None Detected
DNA and RNase: None Detected

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• DNase/ RNase: Not Detected
• EEO: 0.09 - 0.13
• Gelling Point: 34.5 - 37.5 °C
• Gel Strength (1.0%): ≥ 1200 g/cm²
• Moisture Content: ≤ 10.0 %
• Sulfate: ≤ 0.15 %

PR1MA™ Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.

Gel Strength, 4%: ≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Powder
• EEO: ≤ 0.13 -Mr
• Gelling Temperature: 32.5 - 38.0 ^oC
• Gel Strength : ≥ 1400 g/cm²
• Melting Temperature: ≤ 90.0 ^oC
• Moisture Content: ≤ 10.0 %
• Sulfate: ≤ 0.15 %

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.

Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to off white powder
• DNase/ RNase: Not Detected
• EEO: ≤ 0.10 -Mr
• Gelling Temperature: 26 - 30 ^oC
• Gel Strength : ≥ 200 g/cm²
• Melting Temperature: ≤ 65 ^oC
• Moisture Content: ≤ 10 %
• Sulfate Content: ≤ 0.10 %