PR1MA™ Luria Broth Granulated [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

Dissolve 25 grams in 1 liter distilled water and adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Material specially granulated to reduce dust and increase solubility rate.

Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige granular powder
• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

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PR1MA™ PBS / Tween 20 10X Solution

CAS Number: 7558-79-4, 7778-77-0, 7647-14-5, 7447-40-7, 9005-64-5, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, yellow solution
• pH: 7.2 - 7.6

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CAS Number: 56-40-6
Molecular Weight: 75.1
Chemical Formula: C₂H₅NO₂
Solubility: Water
Storage Temp: Room Temperature

For use in preparing electrophoresis buffers. Cell culture tested.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White odorless crystalline powder with sweet taste.
• Solubility (5% in water): Clear & colorless
• Identification (IR): Test Passes
• Loss on Drying (@105 ^oC for 2 hrs): ≤ 0.20 %
• Residue on Ignition: ≤ 0.10 %
• Chloride (Cl): ≤ 0.007 %
• Sulfate: ≤ 0.0065 %
• Heavy Metals: ≤ 0.002 %
• Hydrolyzable Substances: Test Passes
• Related Compound (Diglycine): ≤ 0.1 %
• Related Compound (Triglycine): ≤ 0.1 %
• Related Compound (Glycine Anhydride): ≤ 0.1 %
• Related Compound (Monochloro Acetic Acid): ≤ 0.1 %
• Related Compound (Iminodiacetic Acid): ≤ 0.1 %
• Related Compound (Haxamathylenetetraamine): ≤ 0.1 %
• Related Compound (Total Impurities): ≤ 1.0 %
• Residual Solvent (Chloroform): ≤ 60 mcg/gm
• Residual Solvent (1,4 Dioxane): ≤ 380 mcg/gm
• Residual Solvent (Methylene Chloride): ≤ 600 mcg/gm
• Residual Solvent (Trichloroethylene): ≤ 80 mcg/gm
• Methanol: ≤ 0.3 %
• Assay: 98.5 - 101.5 %
• Arsenic: ≤ 1.0 ppm
• Total Viable Bacterial Count: ≤ 1000 Cfu/gm
• Coliform Count: Negative
• Yeast and Mold Count: ≤ 100 CFU/g
• Test for E-Coli: Absent
• Test for Salmonella: Absent
• Test for S-Aureus: Absent
• Test for Pseudoomonas: Absent
• BSE/TSE Free: BSE/TSE Free
• Grade: USP
• Country of Origin: Report

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PR1MA™ Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.

Suitable for Microbiology Applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Powder
• Amino Nitrogen Content: ≥ 3.5 %
• Dry Matter: ≥ 94 %
• Moisture : ≤ 6.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen Content: ≥ 8.0 %
• Non Animal Origin: The product does not come in contact with materials of animal origin.

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PR1MA™ Luria Agar Powder [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

• Dissolve 40 grams in 1 liter distilled water
• Adjust pH to 7.2
• Sterilize by autoclaving for 15 minutes

Ingredients:

• Peptone: 10g/L
• Yeast: 5 g/L
• Sodium Chloride: 10g/L
• Agar: 15g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (4% solution at 25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ PBS [Phosphate Buffered Saline]

Common Name: Phosphate Buffered Saline
CAS Number: 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temp: Room Temperature

PBS, 100 mL Tablets refer to pre-weighed tablets that are used to prepare a specific volume of PBS (Phosphate Buffered Saline) solution. 

PBS is a widely used buffer solution in biological and biochemical research. PBS serves as a versatile buffer for various applications, including cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. It helps maintain a stable pH and osmolarity, providing optimal conditions for biological reactions and preserving the integrity of cells and biomolecules. 

Each tablet contains pre-determined amounts of sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic. When dissolved in distilled water or a suitable solvent, the tablet dissolves, releasing the components, and instantly forms a ready-to-use PBS solution with a total volume of 100 mL.

These tablets ensure accurate and consistent preparation of PBS, eliminating the need for weighing and measuring individual components. They are designed to simplify the process and save time in laboratory workflows.

Research or further manufacturing use only, not for food or drug use.

Appearance: White, Concave Tablets

Clarity and Color of Solution: Clear colorless solution (No Cloudy) 5 tablets in 500 ml H2O

Hardness Test: Non-Fragile, No Chips, No Broken Tablets

pH (1 Tablet/ 100 ml Water): 7.3 - 7.5

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PR1MA™ 2XYT Broth, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Material is specially granulated to reduce dust and increase solubility rate.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final pH@ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing granulated
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2X Master Mix
• Includes PR1MA™ Hot Start Taq Polymerase for greater specificity and accuracy 

Supplied as a ready-to-use 2X master mix, PR1MA™ qMax Green has been engineered for high sensitivity, fast cycling, and excellent reproducibility. PR1MA™ Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers, and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

*Please note these products ship on dry ice. Appropriate shipping charges apply unless otherwise noted on a quote.

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PR1MA™ Sodium Chloride, Powder

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine White Granular Powder
• Acidity or Alkalinity: Pass Test
• Appearance of Solution: Clear, Colorless Solution
• Arsenic : ≤ 1 µg/g
• Assay: 99.0 - 100.5 %
• Barium: Pass Test
• Bromides: ≤ 0.010 %
• Ferrocyanide: Pass Test
• Heavy Metals : ≤ 5 ppm
• Identification (Chloride): Pass Test
• Identification (Sodium): Pass Test
• Loss on Drying: ≤ 0.5 %
• Magnesium & Alkaline Earth Metals:  0.01 %
• Iron(Fe): ≤ 2 µg/g
• Nitrites: ≤ 0.01
• Phosphates : ≤ 0.0025 %
• Sulfate: ≤ 0.020 %

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PR1MA™ Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
• 1.5~2.5 μL per well for general Western blotting transfer

The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)

PR1MA™ Tricolor Protein Markers

PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μL per well for general Western blotting transfer.

The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Specifications

Item#	Color	Bands	Range
PR5010-BL	Blue	10	9-180 kDA
PR5011-BL	Blue	11	9-180 kDA
PR5010-TC	Tri-Color	10	9-180 kDA
PR5012-TC	Tri-Color	12	9-245 kDA
PR5013-TC	Tri-Color	13	9-245 kDA

 

PR1MA™ Agar, Phyto, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Specially selected and tested agar for use in plant tissue culture. High gel strength.

Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).

Research or further manufacturing use only, not for food or drug use.

• Appearance: Pale Yellow Powder
• Gelling Point: 34 - 36 °C
• Gel Strength : 950 - 1050 g/cm²
• Identification: Conforms
• Loss on Drying: ≤ 18 %
• Melting Point: 85 - 95 ^oC
• Nephelos: ≤ 40 NTU
• pH: 6.0 - 7.5
• Total Ash: ≤ 3.5 %
• Gelatin: Absent
• Starch: Absent
• Total Plate Count: ≤ 1000 cfu/g

PR1MA™ Casamino Acids [Casein acid hydrolysate]

Common Name: Casein acid hydrolysate
CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Casein Acid Hydrolysate is a hydrochloric acid digest of casein that contains small amounts of cystine and Tryptophan. All vitamins have been eliminated by the acidification treatment. Provides a source of amino acids as nutrients for growing various organisms tolerant of high salt conditions.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous, Free Flowing Powder
• Color: Light Tan Yellow
• Amino Nitrogen: ≥ 4.0 %
• Moisture : ≤ 6.0 %
• pH: 6.0 - 7.5
• Protein: Report %
• Solution (2%): Clear Light Yellow
• Total Nitrogen: > 7.5 %
• Coliforms: < 10 cfu/g
• Standard Plate Count : < 5000 cfu/g
• Thermophile Count: Report cfu/g
• Source: Bovine

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