PR1MA™ X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-galactoside
CAS Number: 7240-90-6
Molecular Weight: 408.6
Chemical Formula: C₁₄H₁₅BrCINO₆
Solubility: DMSO and DMF
Storage Temp: -20°C

Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.

Water Content: 0.05% (K. Fisher)
Protect from light and moisture

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Identity: Conforms to structure
• Purity (HPLC): ≥ 98.0 %
• Solubility (2%, DMF): Clear and Colorless Solution
• Specific Rotation: -63.0 - -60.0 °
• Water Content (K.F): ≤ 0.5 %

PR1MA™ Sodium Carbonate Anhydrous, ACS Grade

Common Name: Soda Ash
CAS Number: 497-19-8
Molecular Weight: 105.99
Chemical Formula: Na₂CO₃
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine White Granular Powder
• Calcium (Ca): ≤ 0.03 %
• Chloride (Cl): ≤ 0.001 %
• Heavy Metals: ≤ 0.0005 %
• Insolubles: ≤ 0.01 %
• Iron: ≤ 0.0005 %
• Loss On Heating: ≤ 1.0 %
• Magnesium (Mg): ≤ 0.005 %
• Phosphate: ≤ 0.001 %
• Potassium (K): ≤ 0.005 %
• Purity: ≥ 99.5 %
• Silica: ≤ 0.005 %
• Sulfur Compound: ≤ 0.003 %

PR1MA™  p-Amino Benzoic Acid [Vitamin H1]

Common Name: Vitamin H1
CAS Number: 150-13-0
Molecular Weight: 137.1
Chemical Formula: C₇H₇NO₂
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay: ≥ 98.5 %
• Loss on Drying: ≤ 0.20 %
• Melting Point: 184 - 191 ^oC

PR1MA™  D-(+)-Biotin [Vitamin H]

Common Name: Vitamin H
CAS Number: 58-85-5
Molecular Weight: 244.3
Chemical Formula: C₁₀H₁₆N₂O₃S
Solubility: Water
Storage Temp: 2-8°C

Research or further manufacturing use only, not for food or drug use.

• Appearance: White or off white powder
• Assay: 97.5 - 102.0 %
• Related Substance (HPLC-Individual Impurity): ≤ 1.0 %
• Related Substance (HPLC-Total Impurities): ≤ 2.0 %
• Residual Solvents: To meet requirements
• Specific Rotation: +89 - +93 °
• BSE/TSE Free: BSE/TSE Free
• Grade: USP

PR1MA™ Phleomycin, Powder

CAS Number: 11006-33-0
Molecular Weight: 1525
Chemical Formula: C₅₅H₈₅O₂₁N₂₀S ₂Cu • HCl
Solubility: Water
Storage Temp: 2-8°C

Produced by Streptomyces verticillus and part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent in transformation experiments with mammalian cells, plant cells, and yeast. Cytotoxic action of this type of antibiotic results from the ability to cause fragmentation of DNA. In Vitro studies indicate that Phleomycin causes the accumulation of cells in the G2 phase of the cell cycle.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Blue Powder
• U.V. Absorbance (244nm, 600 µg/ml, 0.1cm Path): 0.20 - 0.30
• Storage Condition: Store at 2 - 8 ^oC (Very Hygroscopic: Keep container tightly sealed until ready for use).

PR1MA™ IPTG [Isopropyl-β-D-thiogalactopyranoside], Dioxane Free

Common Name: Isopropyl-β-D-thiogalactopyranoside
CAS Number: 367-93-1
Molecular Weight: 238.3
Chemical Formula: C₉H₁₈O₅S
Solubility: Water, ethanol
Storage Temp: 2-8°C

IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.

Dioxane-Free
Water Content: <0.49%

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (HPLC): 98.0 - 101.0 %
• Purity (HPLC): ≥ 99.0 %
• 1,4-Dioxane: Not Detected
• Absorbance @300nm (5% w/v sol in H₂O): ≤ 0.13
• Absorbance @400nm (5% w/v solution in H₂O): ≤ 0.06
• Identification: IR Concordant with Standard
• Melting Range: 110 - 114 °C
• Moisture Content (KF): ≤ 1.0 %
• pH (5% in water): 5.0 - 7.0
• Solubility: Soluble in Water and Methanol
• Specific Optical Rotation: -34.5 - -28.5 ^o
• BSE/TSE Free: BSE/TSE Free
• Non Animal Origin: Non Animal Origin
• Country of Origin: Report

PR1MA™ Benzamidine Hydrochloride

CAS Number: 1670-14-0

Molecular Weight: 156.6

Chemical Formula: C7H8N2 • HCl

Solubility: Water

Storage Temp: 2-8°C

Competitive serine protease inhibitor which forms stable complexes with and blocks the active sites of enzymes. Target enzymes include trypsin, hymotrypsin, kallikzein and plasmin.

Shipping Weight: 0.3 lbs

Shipping Dimensions: 3.50 x 3.75 x 3.50

Research or further manufacturing use only, not for food or drug use.

Specifications

• Appearance: White crystalline powder
• Assay (HPLC): ≥ 98.5 %
• Loss on Drying: ≤ 2 %
• Residue on Ignition: ≤ 0.5 %
• Material Source: Chemical Synthesis
• BSE/TSE Free: BSE/TSE Free
• Country of Origin: Report 

PR1MA™ bis-Acrylamide, 2% Solution

CAS Number: 110-26-9
Storage Temp: 2-8°C

A 2% (w/v) bis-Acrylamide solution.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, colorless liquid
• Conductivity of Solution: Report µmhos
• Electrophoresis: Pass Test
• pH: Report

PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder 29:1 Ratio

Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Conductivity of Solution: ≤ 10.0 µmhos
• Electrophoresis: Pass Test
• Solubility: Pass Test

PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder 37.5:1 Ratio

CAS Number: 79-06-1, 110-26-9
Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White powder
• Conductivity of Solution: ≤ 10 µmhos
• Electrophoresis: Pass Test
• Solubility: Pass Test

PR1MA™ Acrylamide/bis-Acrylamide, 37.5:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C

A 40% solution containing 38.96% (w/v) Acrylamide and 1.04% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 37.5:1.

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, colorless solution
• Conductivity of Solution: ≤ 10 µmhos
• Electrophoresis: Pass Test
• pH: Report

PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder 19:1 Ratio

Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• Solubility: Clear and complete
• Conductivity: ≤ 10 µmhos