Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.   

RWD Mouse and Microglia Cell Separation Kits

Mouse CD3+ Cell Separation Kit - K1301-10

For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.

Mouse CD4+ Cell Separation Kit - K1302-10

For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.

Mouse CD8+ Cell Separation Kit - K1303-10

For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.

Mouse CD45+ Cell Separation Kit - K1304-10

For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.

Mouse CD19+ Cell Separation Kit - K1305-10

For positive selection or depletion of B cells from mouse spleen and other samples.

CD11b+ (Microglia) cell separation Kit - K1306-10

For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.

Specifications
 

Item #	Cell Type	Size	Capacity
K1301-10	T Lymphocytes	2 x 1 mL	For 1*10^9 Total Cells
K1302-10	T Helper Cells		For 1*10^9 Total Cells
K1303-10	Cytotoxic T Cells		For 1*10^9 Total Cells
K1304-10	Leukocytes		For 1*10^9 Total Cells
K1305-20	B Cells		For 2*10^9 Total Cells
K1306-10	Myeloid Cells		For 1*10^9 Total Cells

 

PrepGEM™ Universal DNA Extraction Kits

Single-tube, 15-minute DNA extraction solutions.

The PrepGEM™ Universal is a comprehensive DNA extraction kit designed for maximum yield, integrity, and ease of use. Engineered for researchers and technicians who demand efficiency and reliability, this adaptable reagent kit delivers high-quality DNA from both common and challenging sample types, all in a single tube.

Main Attributes

• Single tube simplifies processes, no transfers, no washes, no DNA loss
• Get rapid results from sample to DNA in ~15 minutes
• Broad compatibility optimized for blood, saliva, tissue, and cell culture
• Ideal for CRISPR genotyping and a wide range of downstream analyses
• Reduced plastic waste and hazardous chemicals for sustainable science

Simple and Effortless Extraction from Any Source

Consistently extract high-yield DNA from blood, saliva, tissues (including ear punches, mouse tails, and insects), and cell cultures. Our closed-tube, inhibitor-free method eliminates harsh chemical washes and multiple steps, ensuring no DNA is lost. This makes prepGEM™ Universal exceptionally powerful for low-cell-number extractions, down to a single cell.

Ideal for Advanced Genotyping and Sequencing

PrepGEM™ Universal is the perfect tool for performing confirmatory genotyping of transfected cells after CRISPR/Cas9 gene editing or preparing DNA for downstream applications. The kit produces ready-to-use DNA for STR analysis, PCR, qPCR, whole genome amplification, and amplicon sequencing.

Every Lab Deserves Rapid Results

Activate our proprietary thermophilic proteinase with a simple temperature change. The entire hands-off process is easily automated in a single tube using a standard thermocycler and takes approximately 15 minutes. Free up valuable technician time, reduce lab costs, and significantly cut down on plastic and hazardous waste.

Applications

• Blood Samples
• Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
• Saliva Samples
• Liquid, Buccal swab, Stains, Storage Card (FTA)
• Tissue Samples
• Tissue cultures, including single cell, Animal (scarpings, fat, muscle), Mouse and rat tail tips, Ear tags, Fish fins, Insects 

Kit Components

• PrepGEM™
• Histosolv
• BLUE Buffer
• RED+ Buffer
• ORANGE+ Buffer
• PDQeX Kits Include PDQeX Kit Tubes (XPU0100, XPU0500, XPU1000)

PrepGEM™ Bacterial Enzymatic Kits

Speed and gentle precision for unparalleled insight into the microbial world.

The PrepGEM™ Bacteria enzymatic kit is designed for efficient, high-quality DNA extraction from a vast array of microbial sources. Engineered for microbiologists and metagenomics researchers, this method combines gentle lysis with a closed-tube workflow to preserve DNA integrity and ensure accurate community representation, all in a fraction of the time of traditional methods.

Main Attributes

• Enzymatic combination ensures gentle and efficient lysis while preserving DNA integrity
• Rapid results in a quarter of the time of conventional methods
• True Community Representation: Closed-tube, no-transfer process protects low-abundance species in metagenomic samples
• Broad compatibility from pure cultures to complex environmental samples
• Easily automated for high throughput in a secure, closed system for safety and scalability 

Comprehensive Microbial and Metagenomic Profiling

Efficiently extract DNA from Gram-positive and Gram-negative bacteria, protozoa, archaea, colonies, and liquid cultures. The kit is equally effective for complex samples like biofilms, mucosal swabs, and environmental matrices, including soil, stool, and water. It is the ideal choice for metagenomic studies.

Integrity for Demanding Applications

The gentle, temperature-driven enzymatic lysis uses a unique combination of thermophilic proteinase and mesophilic lysozyme to minimize DNA fragmentation. With no mechanical disruption or transfer steps, you obtain exceptionally intact, high-molecular-weight DNA that accurately represents low-abundance species. The resulting DNA is ready for PCR, qPCR, targeted amplicon sequencing such as 16S rDNA analysis, and other downstream applications.

Accelerated Research Process, 1/4 the Time of Other Methods

The fully enclosed, single-tube system eliminates cross-contamination, protects users from pathogens, and requires minimal hands-on time. The automation-ready workflow reduces pipetting, preserves sample integrity, and drastically cuts down on plastic waste.
 

Applications

• Extraction from a variety of microbial samples including:
• Gram-positive and -negative bacteria, protozoa, colonies and liquid cultures, biofilm and mucosal, archaea, swabs, and metagenomic DNA: soil, stool, water 

Kit Components

• PrepGEM™
• GREEN+ Buffer
• Lysozyme
• WASH+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XBA0100, XBA0500, XBA1000)

ForensicGEM™ Universal Enzymatic Kits

Precise, efficient, and secure solution for modern forensic DNA analysis.

ForensicGEM™ Universal is a DNA extraction kit designed for fast, reliable results in forensic science. Where speed, sample integrity, and minimal contamination are paramount, our innovative enzymatic process delivers analysis-ready DNA from challenging forensic samples in just 15 minutes.

Main Attributes

• Obtain STR, PCR, and qPCR-ready DNA in approximately 15 minutes
• Eliminating purification steps enables efficient extraction from very small sample volumes
• Single-tube, closed processing protects sample integrity and minimizes contamination risks
• Reduced hands-on time, lab costs, consumable use, and plastic waste
• Compatible with standard lab equipment for easy automation and adoption 

Optimized for a Comprehensive Range of Forensic Evidence Types

Consistently extract high-yield DNA from saliva (liquid, swabs, stains, FTA cards), blood (liquid, swabs, stains, FTA cards), and tissue (solid samples and hair). The process is uniquely effective for both small-volume and trace samples.

Superior DNA for Demanding Forensic Analysis

The method produces DNA that is PCR-ready and suitable for STR profiling. Unlike traditional methods, our temperature-controlled process uses a novel cocktail of enzymes instead of harsh detergents and reducing agents like SDS or DTT. This eliminates common PCR inhibitors, ensuring superior results without purification steps.

Fully Hands-Off, Single-Tube Workflow

From sample to analysis, the process is contained, drastically reducing opportunities for contamination and protecting sample integrity. This approach is perfect for high-volume casework where quick turnaround is critical. The method is easily automated using existing thermal cyclers, freeing up valuable technician time and accelerating response times.

For extracting DNA from sperm, use ForensicGEM™ Sperm.
 

Applications

• Extraction from a variety of forensic blood samples
• Liquid (fresh, EDTA, Heparin, Citrate), Swab, Stains, Storage Card (FTA)
• Tissue samples
• Animal (scrapings, fat, and muscle), Tape lifts, Hair
• Saliva samples
• Liquid, Swab, Stains, Storage Card (FTA) 

ForensicGEM™ is a preparative method for DNA extraction. The method lyses cells and removes nucleoproteins from the DNA. Extracted DNA can be used for many types of genotyping, including SNP and STR analysis as well as quantitative, multiplex, and endpoint PCR.

There is no concentration step in the procedure, so the concentration of the extract is dependent on:

• The quality of the sample
• In the case of swabs, the type of swab and the volume of water used to wash the swab
• The extraction volume (which in some cases can be scaled)

DNA extracted using ForensicGEM™ is largely single stranded because of the 95ºC heat step. For accurate yield assessment, a qPCR is recommended. If standard fluorescent chelating dyes are to be used for normalising samples, then we recommend taking a sample of the extract before the 95ºC step. Alternatively, you can generate a standard curve using a previously made extract that has been quantified.

Kit Components

• ForensicGEM™
• BLUE Buffer
• RED+ Buffer
• ORANGE+ Buffer
• Histosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFU0100, XFU0500, XFU1000)

ForensicGEM™ Sperm Enzymatic Kits

Precision and efficiency for forensic differential extraction.

Make the complex challenge of sperm cell lysis fast and simple. ForensicGEM™ Sperm is a specialized enzymatic kit designed for the rapid, single-tube extraction of high-quality DNA from sperm cells, semen samples, and vaginal swabs. Eliminating the need for harsh, inhibitory chemicals and lengthy purification helps achieve superior yields and STR-ready DNA in just 11 minutes.

Main Attributes

• Ultra-fast results, analysis-ready DNA in ~11 minutes.
• No purification or bead/column steps means no DNA loss, enabling work with very small cell numbers
• Inhibitor-free DNA: eliminates harsh chemicals like DTT that interfere with STR and qPCR, removing the need for post-lysis cleanup
• Single-tube, no-transfer processing safeguards results and minimizes the risk of contamination
• Frees technician time, reduces plasticware use, cuts costs, and is easily automated 

Reliable DNA Extraction from Sperm Cells

Sperm cells are notoriously difficult to lyse without compromising downstream analysis. This kit enables efficient differential extraction workflows and Y-screening, even from samples with very low sperm counts. The method produces high-quality double-stranded DNA for STR profiling, PCR, and qPCR.

Free of Downstream Inhibitory Compounds/Chemicals

Traditional methods rely on STR-inhibiting chemicals like DTT, SDS, and mercaptoethanol, which are toxic to downstream processes and require time-consuming purification steps that cause DNA loss. ForensicGEM™ Sperm uses a novel, temperature-controlled cocktail of thermophilic and mesophilic enzymes.

~11-Minute, Hands-Off process Completed in a Single Tube

This closed-tube system eliminates transfer steps, minimizing opportunities for contamination, cross-talk, and human error while protecting sample integrity. The protocol is compatible with existing thermal cyclers and easily automated for high-throughput processing, from low-volume casework to large-scale batches. This rapid processing means sex crime cases can be triaged very quickly to determine if a Y chromosome is present in the sample.

Applications

• Extraction from semen, including:
• Swab, Stain, Liquid 

Kit Components

• ForensicGEM™
• ORANGE+ Buffer
• Acrosolv
• PDQeX Kits Include PDQeX Kit Tubes (XFS0100, XFS0500, XFS1000)

PhytoGEM™ Enzymatic Kits

Robust, automated solution for modern plant science and mycology.

Eliminate the challenges of plant and fungal DNA extraction with phytoGEM™, the specialized kit designed for the automated, high-quality isolation of DNA from even the toughest samples. From resilient leaf tissue to complex fungal pathogens, our enzymatic lysis method overcomes inhibitory compounds and tough cell walls, delivering PCR and qPCR-ready DNA without laborious grinding, columns, or hazardous solvents.

Main Attributes

• Enzymatic lysis efficiently breaks down tough cells, handling plant and fungal cell walls without mechanical grinding
• Produces clean DNA free from polysaccharides and polyphenols that inhibit PCR
• Enables simple, closed-tube processing on the PDQeX system for high consistency and safety
• Accelerates pathogen detection and genetic analysis from hours to minutes for rapid results
• Trusted for key plant diseases and fungal genomics 

Designed for Diverse Botanical and Mycological Samples

Effortlessly extract DNA from a wide variety of plant materials, including leaf, stem, and root tissues, as well as from plant pathogens, fungi, and oomycetes. PhytoGEM™ is the ideal tool for studying plant genotypes, genetic variation, endophytic pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and verifying the authenticity of botanical extracts.

Overcomes Traditional Extraction Challenges

Conventional methods struggle with the polysaccharides, tannins, and polyphenols released from plant and fungal cells, requiring multi-step, time-intensive processes that often degrade DNA. PhytoGEM’s proprietary, temperature-controlled enzymatic lysis effectively breaks down tough cell walls in a closed-tube system. This gentle approach eliminates harsh chemical washes, preserves DNA integrity, and yields inhibitor-free DNA suitable for sensitive downstream applications.

Proactive Disease Control Through Rapid Detection of Critical Pathogens

Early detection is critical for crop management. PhytoGEM™, used with the automated PDQeX Nucleic Acid Extractor, transforms this bottleneck into an easy process. From a simple leaf punch on a storage card or homogenized tissue, the system performs precise, closed-tube lysis and purification, drastically reducing hands-on time and contamination risk.

Validated for Key Agricultural Threats

• The PhytoGEM™ method has been specifically validated for detecting a range of economically significant pathogens, including:
• Bacteria: Xylella fastidiosa, Pseudomonas syringae (Psa), Candidatus Liberibacter solanacearum
• Oomycetes: Phytophthora species
• Phytoplasma: In grapevines
• Viruses: Cassava Mosaic Virus
• Fungi: Providing high-quality DNA for genomics, strain characterization, and environmental studies 

Applications

• Plant samples
• Leaf, Stem, Root, Plant Pathogens, Fungi 

Kit Components

• PhytoGEM™
• Histosolv
• GREEN+ Buffer
• Enhancer
• PDQeX Kits Include PDQeX Kit Tubes (XPP0100, XPP0500, XPP1000)

RNAGEM™ Nucleic Acid Extraction Kits

Effortless, ultra-fast nucleic acid extraction.

RNAGEM™ utilizes Exymes' novel thermophilic proteinase and buffer system to extract total nucleic acids in less than 25 minutes without the need for purification.

• Sample types include cell culture, single-cell, and exosomes
• Downstream applications include PCR, RT-PCR, RT-qPCR, LAMP, RT-LAMP, and Nanostring 

Main Attributes

• Single-tube nucleic acid extraction in less than 25 minutes
• Total nucleic acid extraction
• genomic DNA
• mRNA
• long non-coding RNA
• small non-coding RNA
• No ionic detergents or chaotropic salts
• High nucleic acid recovery, minimal loss of nucleic acids during extraction
• No magnetic beads, no spin-columns
• Suitable for low-throughput to high-throughput extraction with a single protocol
• Easily automated using standard liquid handling solutions
• Minimal plasticware required, reduces waste and supply chain issues 

Applications

• Total RNA extraction from
• Mammalian cells, Virus 

Kit Components

• RNAGEM™
• BLUE Buffer
• DNase I
• Dnase Buffer
• TE Buffer

This brand is being discontinued and will only be available while supplies last.
 

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.
 

Bullseye DNA Safe Stain Plus

• Higher sensitivity
• Use in the same way as EtBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Safest DNA stain by far
• Low cost
• 10,000X
• Excitation wavelengths at 290nm and 490nm

DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.

DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.

Guanidine Hydrochloride

• Appearance: White Crystalline Powder
• Purity: ≥ 99.0%
• Solubility (6M Aqueous): Clear and Colorless
• pH: 5.0 - 7.0
• Identity (FTIR): Conforms to Structure
• Water: ≤ 0.3%
• Heavy Metals (as Fe): ≤ 5 ppm
• A260 (6M Aqueous): ≤ 0.10
• A280 (6M Aqueous) ≤ 0.05
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

IBI Ethanol (Anhydrous Alcohol)

Introducing our premium-grade Ethanol, also known as Anhydrous Alcohol – the versatile solution for a myriad of applications. Sourced and refined with meticulous care, our Ethanol stands as a hallmark of purity and quality, ready to meet your diverse needs.

Our Ethanol is widely used for precipitating nucleic acids. The nucleic precipitate, which is formed in the presence of moderate concentrations of monovalent cations, is recovered by centrifugation and re-dissolved in an appropriate buffer at the desired concentration.

200 Proof Ethanol is denatured with methyl alcohol. It is also known as ethyl alcohol, alcohol anhydrous, denatured alcohol.

Specifications

CAS#: 64-17-5

200 Proof Ethanol denatured with 5% methanol

Used for precipitating nucleic acids

Specific Gravity: 0.7964 Max.
Formula Weight: 46.07
Molecular Formula: C2H5OH
Ethanol: 95%
Methanol: 5%
Moisture (KF): 1% Max.
Identification (IR): Pass

Molecular Biology Specifications

DNase assay: None Detected
RNase assay: None Detected

This product cannot be shipped to a personal residence

T4 DNA Ligase

Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.  This enzyme joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
 

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Intact Genomics T4 DNA Ligase displays up to 3-5X higher ligation efficiency than the nearest competitor.

Product Source
E. coli strain expressing a recombinant clone

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Contents & Storage

1. T4 DNA Ligase
2. 10x T4 DNA Ligase Reaction Buffer (w/o ATP)
3. 10 mM ATP

Store all contents at -20 °C.

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C

10x T4 DNA Ligase Reaction Buffer (w/o ATP) 
500 mM Tris-HCl, 100 mM MgCl, 100  mM DTT, pH 7.5 @ 25 °C

Note
10x T4 DNA ligase buffer does not contain ATP. You need to add ATP separately.

Unit Definition 
One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P from pyrophosphate into Norit-absorbance material in 20 minutes under standard assay conditions.

Protocol

1. Set up reaction buffer in a microcentrifuge tube on ice. Use a molar ratio of 1:3 vector to insert DNA.

1. Gently mix the reaction and centrifuge briefly.
2. For cohesive ends, incubate 16 °C for overnight or at room temperature for 30 min.
3. For blunt ends, incubate 16 °C for overnight or at room temperature for 2 hrs.
4. Heat inactivate at 70 °C for 15 min.
5. Cool on ice and transform 2 µl of the reaction into 50 µl competent cells.

ig-Fusion Cloning Kit 
Intact Genomics propriety ig-Fusion cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high-fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency.

Benefits

• Clone any insert at any site within any vector
• Restriction enzyme and phosphatase free system
• Joining multiple large fragments at once
• Precise insertion at a desired orientation
• Rapid and high efficiency with > 95% positive clones

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Contents & Storage

• 5x ig-Fusion enzyme premix: -20 °C
• 2x PCR premix: -20 °C
• High efficiency competent cells: -80 °C
• Recovery medium:4 °C or -20 °C

Protocol
1. Linearize the vector by restriction enzyme digestion or inverse PCR and purify the product with spin column.
2. Design PCR primers for the gene of interest with 15 to 20 bp at 5'-extensions that are complementary to the ends of the linearized vector.
3. Amplify the gene of interest with Intact Genomics 2x PCR premix or any other high-fidelity DNA polymerase. Run the PCR product on an agarose gel to determine the integrity of the PCR product.
4. Purify the PCR product with spin column.
5. Set up the ig-Fusion cloning reaction as follows: Insert and vector molar ratio 3:1 produce the highest number of colonies.  

6.  Mix the reaction mixture thoroughly.
7.  Incubate the reaction mixture at 50 °C for 10-30 min, then place on ice. Number of colonies depend on the incubation time, insert size and number of inserts need to clone.
8.  Use 2.0 µl of the reaction mixture and transform into high efficiency ig 10B chemical or electroporation competent cells (included). To get the maximum number of colonies, we recommend to use ig 10B electrocompetent cells (Cat # 1212).