Want to provide an environment in vitro as close as possible to in vivo? Try TPP Bioreactors!

TPP TubeSpin® Bioreactors have a proven track record in large scale screening and optimization processes of suspension cells. The optimization of the parameters, important for production up-scaling, can be evaluated with small amounts maximizing the number of parallel experiments at the same time.

Suspension cells ranging from immune cells to insect cells and microorganisms like E.coli to yeast can be cultured and centrifuged in TubeSpin® Bioreactors, eliminating the need for transferring before spinning down.

Features:

• working volume 1-35mL
• easy to start small suspension cell cultures from frozen
• ergonomic use of bacterial and yeast cultures -- static and shaking
• conical bottom
• 0.2µM filter membrane
• Sterile gas exchange
• 5 openings
  • (A, B, C, D, E) of different size above the gas permeable, sterile PTFE filter of the screw cap
• Openings can be sealed to adjust gas exchange according to need
• Fits in standard 15ml centrifuge rotors
• Saves costs of culture start-ups in erlenmyer flasks and then transfer to conical tubes
• Culture and spin in a single tube

Advantages:

• Great gas exchange throughout the culture
• Defined ventilation
• Flexible working volume with one type of tube
• No transfer stage - Spin down in the same tube
• Large-scale parallel experiments
• Rapid sampling following centrifugation, and the tube is disposable
• No internal stirring mechanism to damage cells or produce foam
• Less incubator or hood space needed; when compared to a T75
• Less plastic usage and less plastic waste
• Easier withdrawal from tube than a flask

Bacterial and Microbiological Cultures
TPP TubeSpin Bioreactors are ideally suited for the culture of bacteria, yeast, and other microbiological organisms in suspension.  The simple, disposable format offers convenience and time-savings over traditional glass vessels such as test tubes which must be washed and autoclaved before each use.  Both aerobic and anaerobic cultures can be grown by selectively covering the holes in the 0.22um filter cap.  1-35mL of culture can be grown in each TubeSpin bioreactor, offering the flexibility to perform mini plasmid preps, small-scale candidate screens, or other downstream applications.

The simple 50mL tube design contains no internal mixing or stirring mechanism which minimizes cell shearing and foam formation, and agitation is provided by shaking in a CO2 shaking incubator. In this manner, hundreds of cell cultivations can be performed quickly and efficiently in parallel.

Water loss
If small culture volumes are incubated over a longer period of time, the loss of water influences the growth of cells.

By the closure of one or several openings above the gas permeable and sterile PTFE membrane, this water loss can be minimized.

Sterile gas exchange is guaranteed through the 0.22 µm pore size PTFE filter membrane.

See our NEW 600ml TubeSpin Tube

Please call us for sample!

Literature and comprehensive information about shaking technology can be found in the "Forum Shaking Technology", a cooperation of TPP with competent partner companies.

Instruction for use

Check out our information Flyer

See the latest citations on the TubeSpin Bioreactors, click on their names to view the pdf file:

Literature

Posters

TubeSpin® bioreactor 600: OrbiShake technology for suspension culture of CHO cells.
Dominique T. Monteil, Saroj Ghimire, Giulia Tontodonati, Lucia Baldi, David L. Hacker, and Florian M. Wurm, Laboratory of Cellular Biotechnology, EPFL, CH-1015 Lausanne, Switzerland

TubeSpin® bioreactors: Growth of and recombinant protein production in Sf-9 insect cells
Xiao Shen, Patrik O. Michel, Lucia Baldi, David L. Hacker, and Florian M. Wurm
Laboratory of Cellular Biotechnology, EPFL, CH-1015 Lausanne, Switzerland

Publications

Monteil Dominique et al: Disposable 600-mL orbitally shaken bioreactor for mammalian cellcultivation in suspension. (2013)

Qiuling Xie et al: TubeSpin bioreactor 50 for the high density cultivation of Sf-9 insect cells in suspension. (2011)

Strnad et al: Optimization of Cultivation Conditions in Spin Tubes for Chinese Hamster Ovary Cells Producing Erythropoietin and the Comparison of Glycosylation Patterns in Different Cultivation Vessels (2010)

Maria J. De Jesus et al, TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology, Biochemical Engineering Journal 17 (2004) 217–223

Tibor Anderlei et al, Shaken Bioreactors Provide Culture Alternative Systems Have Evolved to the Point Where Scale-Up Is No Longer an Issue
,GEN_Nov 2009

Martin Jordan et al, A versatile disposable culture system for high throughput screening of process parameters and production cell lines.

Martin Jordan - Poster - A versatile disposable culture system for high throughput screening of process parameters and production cell lines.

Martin Jordan et al, Tools for High-Throughput Medium and Process Optimization

Matthieu Stettler et al, Novel Orbital Shake Bioreactors for Transient Production of CHO Derived IgGs

Florian M. Wurm et al, Disposable orbital shake bioreactor system from ml to 1000 Liter for cell culture - from concept to reality

Florian M. Wurm et al, Disposable TubeSpin® Bioreactor 600 offers superior cell culture performance and larger working volume for suspension cultures of mammalian cells.

Einwegbioreaktoren für die Prozessentwicklung mit Insektenzellen, 3:2009

Kasper et al: Kultivierungsstrategien für Stammzellen, BIOforum 5_2009

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